Knockout Pigs as Recipients for Spermatogonial Stem Cell Transplantation.

Spermatogonial stem cell (SSC) transplantation into the testis of a germ cell (GC)-depleted surrogate allows transmission of donor genotype via donor-derived sperm produced by the recipient. Transplantation of gene-edited SSCs provides an approach to propagate gene-edited large animal models. DAZL is a conserved RNA-binding protein important for GC development, and knockout (KO) causes defects in GC commitment and differentiation.

Targeted Gene Editing in Porcine Germ Cells.

As the genetic mutations driving human disease are identified, there is an increasing need for a biomedical model that can accurately represent the disease of interest and provide a platform for potential therapeutic testing. Pigs are a better model for human disease than rodents because of their genetic and physiological similarities to humans. However, current methods to generate porcine models are both technically challenging and expensive.

Targeted Gene Editing in Porcine Spermatogonia.

To study the pathophysiology of human diseases, develop innovative treatments, and refine approaches for regenerative medicine require appropriate preclinical models. Pigs share physiologic and anatomic characteristics with humans and are genetically more similar to humans than are mice. Genetically modified pigs are essential where rodent models do not mimic the human disease phenotype.

ORGANOMETRIC CHANGES IN THYMUS UNDER THE INFLUENCE OF PROPYLENE GLYCOL.

The scientific interest in the influence of xenobiotics on the human body is due to the fact that immune organs are characterized by a pronounced response to the influence of endogenous and exogenous factors. Recently, the immunological impairment, as a manifestation of reactions to ecopathogenic conditions, suggests a major pathogenesis role in the development of cardiovascular, neuropsychiatric diseases, as well as diffuse diseases of connective tissue. Objectives - experiment was designed to elucidate the organometric changes in thymus of male rats due to impact of propylene glycol.