Selection and characterization of human scFvs targeting the SARS-CoV-2 nucleocapsid protein isolated from antibody libraries of COVID-19 patients.

In 2019, the novel SARS-CoV-2 coronavirus emerged in China, causing the pneumonia named COVID-19. At the beginning, all research efforts were focused on the spike (S) glycoprotein. However, it became evident that the nucleocapsid (N) protein is pivotal in viral replication, genome packaging and evasion of the immune system, is highly immunogenic, which makes it another compelling target for antibody development alongside the spike protein.

Did COVID-19 change the political communication of polarizing leaders? The case of Salvini's campaigning before and after the pandemic.

In recent years, political polarization saw a significant rise in many political systems. This revamped a scientific debate sparked decades ago, with different schools of thought debating on dynamics, factors, and causes of polarization itself. By looking at political elites' polarizing strategy-one of the factors on which various theories seem to converge-this article tackles the question concerning the impact of the COVID-19 pandemic in terms of political communication.

Recipes for Inducing Cold Denaturation in an Otherwise Stable Protein.

Although cold denaturation is a fundamental phenomenon common to all proteins, it can only be observed in a handful of cases where it occurs at temperatures above the freezing point of water. Understanding the mechanisms that determine cold denaturation and the rules that permit its observation is an important challenge. A way to approach them is to be able to induce cold denaturation in an otherwise stable protein by means of mutations.

Insomnia Prevalence among Italian Night-Shift Nurses.

Background: Insomnia is one of the major health problems related with a decrease in quality of life (QOL) and also in poor functioning in night-shift nurses, that also may negatively affect patients' care. The aim of this study is to evaluate the prevalence of insomnia in night shift nurses. This observational online web-based survey using Google modules specifically aimed to investigate the prevalence and risk factors for insomnia among Italian nurses.