Publications by authors named "A Bernole"
The construction of large-scale databases of molecular profiles of plant varieties for variety identification and diversity analyses is of considerable interest. When varieties of an allogamous species such as oilseed rape are analysed and described using molecular markers such as microsatellites, care is needed to represent the variety in a meaningful yet useful way. It is possible to characterise such heterogeneous genotypes by analysing bulked samples comprising more than one individual seed or plant, but this approach may result in complex microsatellite profiles.
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- The study generated a comprehensive set of genetic markers, including 506 EST-derived, 313 SSR, and 26 BAC end-derived markers, through PCR and analysis of a Cabernet Sauvignon BAC library.
- An electronic PCR approach utilizing grapevine UniGene data yielded 1,140 positive results for 638 different markers, enhancing the understanding of regulatory sequences around crucial genes.
- The research identified 1,731 overlapping BAC clone islands, aiding in linking genetic and physical maps, and indirectly positioning 93 ESTs on the genetic map, which helps assess the genetic map's quality.
Genome projects were initiated on grapevine (Vitis vinifera L., 2n=38, genome size 475 Mb) through the successful construction of four bacterial artificial chromosome (BAC) libraries from three major cultivars, Cabernet Sauvignon (Cabernet S), Syrah and two different clones of Pinot Noir (Pinot N). Depending on the library, the genome coverage represented 4.
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