HIF-1α immunohistochemical expression in decidual cells, villous and extravillous trophoblast in placentas from pregnancies complicated with preeclampsia.

Etiology of preeclampsia (PE) remains unknown despite it being the leading cause of maternal and neonatal morbidity and mortality worldwide. PE placentas show signs of chronic oxidative stress, therefore we set to investigate the HIF-1α immunohistochemical expression in PE placentas. For the first time presented study reported on significantly higher HIF-1α immunohistochemical expression in all of the placental segments; villous trophoblast (VTB), decidual cells (DC) and extravillous trophoblast (EVTB) in the PE placentas compared to placentas from normal pregnancies.

Expression of EGF, EGFR, and proliferation in placentas from pregnancies complicated with preeclampsia.

Objective: To investigate proliferation, EGF and EGFR expression of villous trophoblast (VTB), decidual cells (DC), and extravillous trophoblast (EVTB) in the placentas from pregnancies complicated with preeclampsia (PE) and to compare them with placentas from normal pregnancies.

Methods: Twenty-nine PE placentas and 19 control placentas were studied for EGF and EGFR immunohistochemical expression (noted as week, moderate or strong). Proliferation was expressed as the proliferation index.

One-electron oxidation of antioxidants: a kinetic-thermodynamic correlation.

The values of the bimolecular rate constants for the reactions of 2,2'-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) radical cation with epicatechin (((2.4 ± 0.2)) s(-1) M(-1)), and epigallocatechingallate ((29 ± 5) s(-1) M(-1)) were obtained by spectrophotometric measurements.

Evaluation of the Hg2+ binding potential of fulvic acids from fluorescence excitation-emission matrices.

The effect of Hg(2+) on the fluorescence intensity of three fulvic acids (Pahokee Peat, Pony Lake and Suwannee River) was studied. The fluorescence intensity decreased in the presence of added Hg(2+), while the fluorescence lifetimes were independent of the concentration of Hg(2+) in solution. These results are indicative of ground-states association between the fulvic acids and Hg(2+) with formation of stable non-fluorescent complexes (static quenching process).

Photoinduced reduction of divalent mercury by quinones in the presence of formic acid under anaerobic conditions.

The laser flash photolysis technique (λ(exc)=355 nm) was used to investigate the mechanism of the HgCl(2) reduction mediated by CO(2)(-) radicals generated from quenching of the triplet states of 1,4-naphthoquinone (NQ) by formic acid. Kinetic simulations of the experimental signals support the proposed reaction mechanism. This system is of potential interest in the development of UV-A photoinduced photolytic procedures for the treatment of Hg(II) contaminated waters.