Metabolic status of 1088 patients after renal transplantation: assessment of twelve years monitoring in Algiers Mustapha Hospital.

Since the introduction of monitoring levels of immunosuppressive medications in our service in July 2000, 1088 kidney transplant patients were received for therapeutic drug monitoring and regular follow-up. The aim of this study was to retrospectively analyze the data on these renal graft patients in Algeria and correlate with our 12 years' experience with calcineurin inhibitor (CNI) measurements. In addition, during this period, we also examined other bioche-mical parameters.

Monitoring of C0 and C2 blood concentrations of cyclosporine on the Roche Hitachi 902 analyzer.

This work provided parameters to perform the widely used enzyme multiplied immunoassay technique (Emit) cyclosporine assay on a Hitachi 902 analyzer. Instrument settings were optimized to arrive at assay characteristics regarding precision, linearity, and lower limit of quantitation among 105 samples from renal transplant recipients receiving cyclosporine. We compared successive results with Emit on a Cobas Integra 400 analyzer with HPLC-UV.

[The EMIT Tacrolimus assay: development of application protocol for Roche Hitachi 902 analyzer].

Tacrolimus (FK506, Prograf) is a potent macrolide immunosuppressant used for prevention of organ transplant rejection following transplantation. It has been clear for many years that, as a drug with a low therapeutic index and high inter-and intrapatients pharmacokinetic variability, it requires individualized monitoring of blood concentration. Several methods have been developed to monitor tacrolimus; immunoassays, bioassays and LCMSMS.

[The biochemical diagnosis of Gaucher disease].

Gaucher disease is a disease of overload lyosomale which we often met since a score of years. Since 1980 we had to answer several requests for diagnosis of this metabolic disease. Requests emanating primarily from paediatric services.

[Evaluation of the assay of serum fructosamine in the surveillance of diabetes in children and adolescents].

Serum fructosamine and glycosylated haemoglobin were measured 4 times over 2 month period (1st, 15th, 30th and 60th days) in 40 young diabetics and in 26 controls. A positive correlation was found between these two parameters at the 15th, 30th and 60th days (0.44 less than r less than 0.