[Fluorescent evaluation of blood nuclear cells in experimental myocardial infarction].

Functional (synthetic) activity of acridine orange-stained blood nuclear cells was studied by microspectral fluorescent analysis in experimental myocardial infarction. A specific time course of changes in the synthetic activity of blood nuclears, described by alpha parameter, was detected in uneventful myocardial infarction and infarction complicated by suppurative inflammation of the suture. This time course reflected the reparative changes in the myocardium.

A retinyl palmitate model of the phenomenon of the intrinsic fluorescence increase in ceroid-lipofuscin cytosomes.

We report here on a retinoid model of the phenomenon discovered in 1980, in which ceroid-lipofuscin cytosomes (CLC) increased their intrinsic fluorescence when exposed to fluorescence-exciting (lambda(ex)=365nm) ultraviolet (UV) light. We modeled this effect in vitro, irradiating a methanolic solution of retinyl palmitate (RP) either synthetic or extracted from the aged rat liver retinoid. Following our model, the mechanism of this phenomenon can be explained by the photodecomposition of RP during fluorescence.

[Accumulation of lipofuscin granules in acute myocardial infarct as a model of cell aging].

Electron microscopic investigation of lipofuscin granules (LG) accumulation during one month in the myocardium of young rabbits in case of experimental acute infarction was performed. Accumulation of LG in cardiocytes of the ischemia-damaged myocardium area at the prenecrotic and necrotic stages was found. Analogous LG accumulation in cardiocytes in the perinecrotic area at the stage of infarction development was also observed.

[Microspectrofluorometric analysis of the effect of centrophenoxine on lipofuscin granules of hybridoma (Retrovirus-transformed) cells].

The increase of the own luminescence lipofuscin granules (LG) in the course of single and repeated ultraviolet (UV) excitations (365 nm) in hybridoma (retrovirus transformed) cells cultured with or without 5 x 10(-4) M centrophenoxine (CP) was studied by microspectrofluorometric method. It was shown that CP influences only the rate of photochemical changes of chromophores in LG. Kinetic patterns of the own luminescence intensity of LG at the wavelength of 540 nm during excitation by UV permit one to suggest the occurrence of the cyclic chromophore changes.

[The detection of lipofuscin in a culture of hybridoma cells].

Lipofuscin granules (LG) are found in the cultured hybridoma cells producing monoclonal antibodies to phage. LG have been studied using light and electron microscopy. Luminescent spectra of LG clusters in hybridoma cells are presented.