Probing Native CB2 Receptor Mobility in Plasma Membranes of Living Cells by Fluorescence Recovery After Photobleaching.

In this study, we employed a novel fluorescent probe, RO7304924-which selectively targets cannabinoid 2 receptor (CB2R)-to assess the lateral mobility of CB2R within the plasma membrane of Chinese hamster ovary cells stably expressing a functional, untagged receptor variant. Utilizing confocal fluorescence recovery after photobleaching (FRAP), we quantified the diffusion coefficient and mobile fraction of CB2R, thereby demonstrating the efficacy of RO7304924 as an innovative tool for elucidating the dynamics of this major endocannabinoid-binding G protein-coupled receptor. Our present findings highlight the potential of combining advanced ligand-based fluorescent probes with FRAP for future investigations into the biochemical details of CB2R mobility in living cells, and its impact on receptor-dependent cellular processes.

Psychometric Evaluation of the Heterosexist Harassment, Rejection, and Discrimination Scale: Identification of a Unidimensional 11-Item Scale.

Despite the Heterosexist Harassment, Rejection, and Discrimination Scale (HHRDS) being a routinely used measure of discrimination, there is no current consensus regarding its fundamental psychometric properties. This study sought to: (a) test the dimensionality of the scale using a classical test theory approach, and (b), if lacking a clear multidimensional structure, use an item response theory approach to develop a concise unidimensional measure and examine differential item and test functioning across various identity factors. In a sample of sexual minority adults ( = 1,243), evaluation of the HHRDS supported a reduced 11-item unidimensional measure.

Oxygen sensing in the kidney.

The kidneys fulfil several essential homeostatic functions for the body. One of them is the maintenance of sufficient oxygen supply to the organs. For this purpose, the kidneys control the formation of red blood cells by the production of the hormone erythropoietin.

Guidelines for managing and using the digital phenotypes of pluripotent stem cell lines.

Each pluripotent stem cell line has a physical entity as well as a digital phenotype, but linking the two unambiguously is confounded by poor naming practices and assumed knowledge. Registration gives each line a unique and persistent identifier that links to phenotypic data generated over the lifetime of that line. Registration is a key recommendation of the 2023 ISSCR Standards for the use of human stem cells in research.