Mapping of Prion Structures in the Yeast Rnq1.

The Rnq1 protein is one of the best-studied yeast prions. It has a large potentially prionogenic C-terminal region of about 250 residues. However, a previous study indicated that only 40 C-terminal residues form a prion structure.

A Split-Marker System for CRISPR-Cas9 Genome Editing in Methylotrophic Yeasts.

Methylotrophic yeasts such as and (sin. and , respectively) are commonly used in basic research and biotechnological applications, frequently those requiring genome modifications. However, the CRISPR-Cas9 genome editing approaches reported for these species so far are relatively complex and laborious.

[Surgical treatment of patients with colostoma].

Results of treatment of postcolostomy patients are analyzed. In 74.7% cases colostoma was created due to tumor of the rectum or colon, in 6.

[Appliance of "LigaSure" generator in hemorrhoidectomy].

Results of hemorrhoidectomy in 185 patients were analyzed. In 82 patients of the study group surgery was performed with "LigaSure" generator. Control group consisted of 103 patients who underwent surgery by Milligan-Morgan method.