Branched-chain amino acids (BCAAs) play an important role in the functioning of mammalian cells and the central nervous system. However, available genetically encoded indicators for BCAAs are based on Förster resonance energy transfer and have a limited dynamic range. We developed a single fluorescent protein-based sensor for BCAAs, called NeIle, which is composed of circularly permutated mNeonGreen protein inserted into the leucine-isoleucine-valine binding protein (LIVBP) from bacteria.
View Article and Find Full Text PDFComput Struct Biotechnol J
December 2024
The advent of advanced coatings has transformed material functionalities, extending their roles from basic coverage and visual appeal to include unique properties such as self-healing, superior hydrophobicity, and antimicrobial action. However, the traditional dependency on petrochemical-derived materials for these coatings raises environmental concerns. This study proposes the use of renewable and alternative materials for coating development.
View Article and Find Full Text PDFUnlabelled: Hydrothermal pretreatments are commonly employed prior to the biotechnological conversion of lignocellulosic biomass (LCB) into value-added products, such as fuels and chemicals. However, the by-products of this pretreatment, including furaldehydes, lignin-derived phenolics, and carboxylic acids, can inhibit the enzymes and microbes used in the biotechnological process. In this study, LCB degrading enzymes of endophytic and litter fungi were screened for their tolerance to potential pretreatment-derived inhibitors.
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