Publications by authors named "A Antanasijevic"
Because of high mutation rates, viruses constantly adapt to new environments. When propagated in cell lines, certain viruses acquire positively charged amino acids on their surface proteins, enabling them to utilize negatively charged heparan sulfate (HS) as an attachment receptor. In this study, we used enterovirus A71 (EV-A71) as the model and demonstrated that, unlike the parental MP4 variant, the cell-adapted strong HS-binder MP4-97R/167 G does not require acidification for uncoating and releases its genome in the neutral or weakly acidic environment of early endosomes.
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- Congenital myasthenic syndrome-22 (CMS22) is a rare genetic condition linked to variations in the PREPL gene, with previous research focusing mainly on deletions and nonsense mutations.
- This study investigates missense variants in PREPL from three CMS22 patients, revealing that these variants do not affect hydrolase activity, which contradicts existing diagnostic standards.
- Structural analysis indicates that these missense variants interfere with protein interactions and highlight the significance of PREPL's nonhydrolytic functions, suggesting that CMS22 can arise from different types of genetic changes beyond just deletions.
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- Immunodominance of antibodies targeting non-neutralizing epitopes and high somatic hypermutation in germinal centers (GCs) are major challenges in developing a successful HIV vaccine.
- The study utilized implantable osmotic pumps to deliver epitope-targeted immunogens to rhesus macaques, aiming to enhance antibody responses against the conserved fusion peptide over six months.
- Results indicated that while antibody responses against the FP/N611 glycan hole were primed, they showed limited neutralization breadth, and cryoEMPEM revealed key residue information to guide future vaccine design.
Vaccination strategies against HIV-1 aim to elicit broadly neutralizing antibodies (bnAbs) using prime-boost regimens with HIV envelope (Env) immunogens. Early antibody responses to easily accessible epitopes on these antigens are directed to non-neutralizing epitopes instead of bnAb epitopes. Autologous neutralizing antibody responses appear upon boosting once immunodominant epitopes are saturated.
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- - The study investigates how specific amino acid changes in the capsid proteins of coxsackievirus B5 (CVB5) influence the virus's resistance to disinfectants like chlorine and heat treatment.
- - It was found that while the amino acid changes did not significantly affect chlorine sensitivity, some changes at the C-terminal region of viral protein 1 reduced the virus's sensitivity to heat.
- - Cryo-electron microscopy showed that these changes impact the virus's structure, possibly enhancing its stability and affecting how it uncoats during infection, which might explain the reduced heat sensitivity.