Protein Expression Platforms and the Challenges of Viral Antigen Production.

Several protein expression platforms exist for a wide variety of biopharmaceutical needs. A substantial proportion of research and development into protein expression platforms and their optimization since the mid-1900s is a result of the production of viral antigens for use in subunit vaccine research. This review discusses the seven most popular forms of expression systems used in the past decade-bacterial, insect, mammalian, yeast, algal, plant and cell-free systems-in terms of advantages, uses and limitations for viral antigen production in the context of subunit vaccine research.

Porvac subunit vaccine induces neutralizing antibodies against all three main classical swine fever virus genotypes.

Classical swine fever (CSF) is endemic in Cuba and is one of the major health problems of the Cuban swine industry. The current efforts to control the disease in Cuba include vaccination with Porvac, a subunit marker vaccine. Although the efficacy of Porvac against CSF virus (CSFV) subgenotype 1.

Development and Validation of an Indirect and Blocking ELISA for the Serological Diagnosis of African Swine Fever.

African swine fever (ASF) is an economically devastating viral disease of pigs caused by the ASF virus (ASFV). The rapid global spread of ASF has increased the demand for ASF diagnostics to be readily available and accessible. No commercial ASF enzyme-linked immunosorbent assay (ELISA) kits are manufactured and licensed in North America.

Genotype II Live-Attenuated ASFV Vaccine Strains Unable to Completely Protect Pigs against the Emerging Recombinant ASFV Genotype I/II Strain in Vietnam.

: African swine fever virus (ASFV) continues to spread globally, causing severe economic losses to pig farmers. Vietnam licensed two live attenuated vaccines based on the ASFV strains ASFV-G-ΔI177L and ASFV-G-ΔMGF to control the ongoing ASF outbreaks. In 2023, newly emerging highly virulent recombinant ASF viruses (rASFV I/II) containing genetic elements from both p72 genotype I and II ASF viruses were reported from Northern Vietnam.

Development of a Multiplex Real-Time PCR to Disambiguate within Complex, the Proven Vector of Bluetongue and Epizootic Hemorrhagic Disease Viruses in North America.

Species delimitation of complex species can be challenging. Among species within the complex, is considered the primary vector of bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) in North America. Morphological identification of within the .