Appl Biochem Biotechnol
December 2020
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View Article and Find Full Text PDFAppl Biochem Biotechnol
December 2020
Acrylamide/chitosan-based cryogel was fabricated, and a triazine dye, Reactive Green 5, was attached to the cryogel by nucleophilic substitution to build a dye affinity support for adsorption of catalase enzyme. Characterization of cryogel was performed using FTIR, SEM, EDX, BET, and swelling test. Synthesized cryogel beared pores with ~ 200 μm in size and the surface area of 11.
View Article and Find Full Text PDFEnzyme immobilization appears as a remarkable technique to safely attach enzymes for several applications and cryogels stand as promising support materials to be used in such investigations. In this work, papain enzyme was immobilized onto an interpenetrating network obtained by cryogelation of N,N'-methylenebisacrylamide cross-linked 2-hydroxyethyl methacrylate and glutaraldehyde cross-linked chitosan. Cryogels were modified with -NH functionality and glutaraldehyde in order to attach papain covalently.
View Article and Find Full Text PDFArtif Cells Nanomed Biotechnol
April 2014
Molecularly imprinted PHEMAH cryogels were synthesized and used for purification of carbonic anhydrase from bovine erythrocyte. Cryogels were prepared with free radical cryopolymerization of 2-hydroxyethyl methacrylate and methacryloylamido histidine and characterized by swelling degree, macroporosity, FTIR, SEM, surface area and elemental analysis. Maximum carbonic anhydrase adsorption of molecularly imprinted PHEMAH cryogel was found to be 3.
View Article and Find Full Text PDFColloids Surf B Biointerfaces
May 2012
In this study, single-use graphite electrodes modified with 5-amino-2-mercapto-1,3,4-thidiazole (AMT) were fabricated for electrochemical monitoring of DNA. The surfaces of AMT modified pencil graphite electrodes (PGEs) were characterized by scanning electron microscopy (SEM). Electrochemical behaviors of these electrodes were investigated using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS).
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