In unrelated stem-cell transplantation, the value of matching at the HLA-A, -B and -DR loci between donor and recipient is well documented. The effect of HLA-C, DPB1 and DPA1 mismatches on transplantation outcome is unclear. In this study, 104 donor recipient-pairs, transplanted at Huddinge University Hospital between 1988 and 1999, were retrospectively HLA class I- and class II-typed by PCR-SSP.
View Article and Find Full Text PDFDPB1 is the second most polymorphic class II locus with currently 84 recognized alleles, i.e. DPB1*0101 to DPB1*8101.
View Article and Find Full Text PDFAmino acid 57 of DQ beta chains is of functional importance as it influences peptide binding, is part of B and T cell epitopes, and is associated with susceptibility and resistance to insulin-dependent diabetes mellitus and humoral immunodeficiencies. Polymorphism of codon 57 is conserved in primates and in HLA class II B genes implying that balancing selection operates on this residue. Previously, three DQB1 allele pairs have been described, that only differ at residue 57.
View Article and Find Full Text PDFIn the present study PCR primers were designed for detecting all known DPA1 variability, i.e., the presently recognized six DPA1 alleles 0103 to 0401, and also for separation of the four DPA1*02 alleles, by PCR amplification with sequence-specific primers (PCR-SSP).
View Article and Find Full Text PDFEur J Immunogenet
December 1994
In the study described here primers were designed for DQB1 'low-resolution', i.e. generic, typing by PCR amplification with sequence-specific primers (PCR-SSP) considering all the currently recognized DQB1 alleles, i.
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