Additional antilipoperoxidant activities of alpha-tocopherol and ascorbic acid on membrane-like systems are potentiated by rutin.

The effects of alpha-tocopherol, ascorbic acid and rutin on peroxidative processes were studied in xanthine-xanthine oxidase system, linoleic acid ufasomes and human erythrocyte membranes. In these three systems, tested compounds scavenge superoxide anion radicals or inhibit lipid peroxidation in a concentration-dependent manner, and it was shown that rutin was the most potent radical scavenger, followed by ascorbic acid and alpha-tocopherol. An important antilipoperoxidant activity was observed when these compounds were tested in combination, demonstrating that a dose-dependent interaction occurs.

Comparison of the protective effect of various flavonoids against lipid peroxidation of erythrocyte membranes (induced by cumene hydroperoxide).

An experimental model system was designed to test the antioxidant effects of various pharmacologic compounds. Cumene hydroperoxide induces in vitro the peroxidation of erythrocyte membrane and the subsequent formation of malonaldehyde and fluorescent lipid-soluble products. The protective effect of various flavonoids was compared to that of butylated hydroxytoluene (BHT).