[Analysis of SYT/SSX1 and SYT/SSX2 fusion genes from synovial sarcoma].

The t(X;18)(p11;q11) translocation has been shown to be the specific alteration for synovial sarcomas. The translocation leads to production of chimeric protein SYT/SSX by fusion of SYT and SSX genes involved. The expression analysis of SYT/SSX1 and SYT/SSX2 chimeric transcripts was performed in formalin-fixed soft tissue tumour specimens and the diagnostic validity of immunohistochemistry, FISH and RT-PCR methods was compared.

[Study of the HER2 status. Methodological aspects].

The paper deals with the problems of HER2 testing in breast cancer. It describes the optimal immunohistochemical procedure and the principles of assessment of its results.

[Molecular diagnosis of liposarcomas: identification of the chimeric genes FUS/CHOP and EWS/CHOP].

This paper presents the results of an analysis the chimeric genes FUS/CHOP and EWS/CHOP in patients diagnosed as having liposarcoma in order to make a differential diagnosis in both soft tissue tumors and various variants of liposarcoma. Liposarcomas were found in 5 of 7 cases of primary tumors: 4 chimeric transcripts of the FUS/CHOP type (5-2), a variant of alternative splicing of the FUS/CHOP type (5-2) with depletion in 14 p.n.

[Molecular biological approaches to differential diagnosis of skin melanoma].

Twenty-two surgical biopsy specimens from patients with pigmented skin neoplasms were studied. The patients were divided into 3 groups: 1) 11 patients who had a histological verified diagnosis of melanoma; 2) 10 patients who were diagnosed as having nevi with lentiginous melanocytic dysplasia; 3) 1 patient who had an unclear diagnosis of presumptively, nevus with melanocytic dysplasia. By applying fluorescence in situ hybridization (FISH) by means of a Vysis LSI RREBI/LSI MYB/LSI CCNDI/ CEP 6 probe, the authors employed to test a new probe designed for the genetic diagnosis of melanoma by FISH studies and confirmed in all the 22 patients the diagnosis previously made from the results of the routine histological study and the presumptive diagnosis in 1 patient.

[Comparative investigations of the determination of the HER-2 status in breast cancer by fluorescence and chromogenic in situ hybridization].

Forty invasive breast ductal carcinoma biopsy specimens were investigated. The techniques of fluorescence and chromogenic in situ hybridization were used to study HER-2/neu gene amplification and the concordance of these techniques was examined. The advantages and disadvantages of both techniques are shown.