Hemin-Induced Transient Senescence Via DNA Damage Response: A Neuroprotective Mechanism Against Ferroptosis in Intracerebral Hemorrhage.

Intracerebral hemorrhage (ICH) poses acute fatality and long-term neurological risks due to hemin and iron accumulation from hemoglobin breakdown. Our observation that hemin induces DNA double-strand breaks (DSBs), prompting a senescence-like phenotype in neurons, necessitating deeper exploration of cellular responses. Using experimental ICH models and human ICH patient tissue, we elucidate hemin-mediated DNA damage response (DDR) inducing transient senescence and delayed expression of heme oxygenase (HO-1).

Fertility of N'dama and Bunaji cattle to artificial insemination following oestrus synchronization with PRID and PGF2alpha in the hot humid zone of Nigeria.

A study was undertaken to determine the effectiveness of a progesterone-releasing intravaginal device (PRID) and prostaglandin F2 alpha (PGF2alpha) in synchronizing oestrus in N'dama and Bunaji cows and heifers and the fertility following artificial insemination at the synchronized oestrus. A total of 116 cows and heifers (58 N'dama and 58 Bunaji) were used in two separate trials. In the first trial, oestrus was synchronized using a PRID, which was inserted for 12 days; in the second trial, oestrus was synchronized by giving two injections of PGF2alpha 13 days apart.

Fertility of Bunaji (zebu) cows after treatment with PRID with or without PGF2alpha.

A study was undertaken to determine the oestrus response and fertility rates of zebu cows treated with PRID alone or in combination with PGF2alpha. A total of 184 non-suckled cycling Bunaji cows were allotted randomly to four treatment groups of 46 animals per group as follows: group 1 (PRID-12), PRID was inserted for 12 days; group 2 (PRID+7+PGF2alpha-6, PRID was inserted for 7 days and PGF2alpha was administered intramuscularly 1 day prior to PRID withdrawal; group 3 (PRID-7+PGF2alpha-7, PRID was inserted for 7 days and PGF2alpha was administered intramuscularly on the day of PRID withdrawal; group 4 (2 x PGF2alpha-13, two intramuscular injections of PGF2alpha 13 days apart. At the end of each treatment period, the cows were observed for 7 days for behavioural oestrus and were inseminated 12 h following detection of oestrus.

Detection of oestrus in Bunaji cows under field conditions.

The objective of this study was to determine the efficacy of some aids for detecting oestrus in 72 Bunaji cows synchronized using two injections of prostaglandin F2 alpha (PGF2 alpha) 13 days apart. Blood samples to determine the accuracy of the aids and ovarian activity by radioimmunoassay technique were collected daily from the day of the second PGF2 alpha injection until oestrus or for 168 hours for 'non-responders'. The aids for detecting oestrus, tail painting, KaMar detectors and a chin-ball mating device (CMD) were applied at the time of the second injection.