[The efficacy of simultaneous and sequential irradiation and hyperthermic treatment of tumor cells in vitro and transplantable tumors in vivo].

The elevated efficacy of simultaneous treatment of cancer by irradiation and hyperthermia in comparison with their sequential application was confirmed in the experiments with V-79 cells in vitro and transplantable melanoma B-16 and ELD solid carcinoma in vivo. Irradiation of the tumors transplanted in the calf muscle of mice was performed at the extended Bragg peak of accelerated protons, while extremities of the group of 6 animals were fixed one after another in a water bath at 42 degrees C (hyperthermia) or at 36 degrees C (irradiation at normal temperature). In another series of experiments, the tumors were heated with the microwave (2450 MHz) antennae and irradiated on the 60Co clinical machine.

[High-dose gamma-irradiation enhance expression of transgene under control of immediate-early CMV promoter in stably transfected tumor cells].

Gamma-irradiation is a usual method to inactivate whole-cellular anticancer vaccines consisting viable tumor cells. To evaluate the effect of gamma-irradiation to transgene expression in tumor cells we constructed several stably transfected clones of human and mouse cell lines expressing transgenic GM-CSF or GFP under control of IE-CMV promoter. Irradiation of those cells with different doses (ranged from 20 to 100 Gr) of gamma-radiation caused loss of proliferation capacity with survival of the cells population clearly depended on irradiation dose.

[Combined treatment for rectal cancer using different radiomodification means].

The paper presents an evaluation of the results of surgical and combined treatment received by 961 patients at the Center's Clinics within 28 years. The analysis was based on 3 protocols of prospective randomized study. It dealt with assessment of the end results of surgical and combined treatment for operable rectal cancer using one preoperative hyperfractonated STD of 5-25 Gy.