The Russian Far East is a region of unique biodiversity, with numerous plant species, including and . These plants are considered a serious threat to biodiversity and are classified as threatened species. However, plants synthesize and accumulate a variety of metabolites that are valued for their positive effects on human health.
View Article and Find Full Text PDF, alternatively known as or , is a perennial herb belonging to the Polygonaceae family. Commonly called Japanese knotweed or Asian knotweed, this plant is native to East Asia, particularly in regions such as Korea, China, and Japan. It has successfully adapted to a wide range of habitats, resulting in it being listed as a pest and invasive species in several countries in North America and Europe.
View Article and Find Full Text PDFRNA interference (RNAi) is a regulatory and protective mechanism that plays a crucial role in the growth, development, and control of plant responses to pathogens and abiotic stresses. In spray-induced gene silencing (SIGS), exogenous double-stranded RNAs (dsRNA) are used to efficiently regulate target genes via plant surface treatment. In this study, we aimed to evaluate the effect of specific exogenous dsRNAs on silencing different regions (promoter, protein-coding and intron) of the target tomato gene, encoding an R3-type MYB repressor of anthocyanin biosynthesis.
View Article and Find Full Text PDFThe application of double-stranded RNAs (dsRNAs) to plant surfaces has emerged as a promising tool for manipulating gene expression in plants and pathogens, offering new opportunities for crop improvement. While research has shown the capability of exogenous dsRNAs to silence genes, the full spectrum of their impact, particularly on the intricate network of microRNAs (miRNAs), remains largely unexplored. Here, we show that the exogenous application of chalcone synthase ()-encoding dsRNA to the rosette leaves of induced extensive alterations in the miRNA profile, while non-specific bacterial neomycin phosphotransferase II () dsRNA had a minimal effect.
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