Publications by authors named "A A Solovjev"
Article Synopsis
- A new assay was developed to measure the activity of the nicking endonuclease (NE) Nt.Bst9I using biotinylated DNA oligonucleotides as a substrate.
- To enhance the assay's sensitivity, a chemiluminescent detection system was implemented, utilizing a streptavidin-polyperoxidase conjugate and an enhanced chemiluminescence reaction.
- The assay methods were designed for microtiter plates, facilitating automated analysis with ELISA equipment, and the heterogeneous format proved to be more sensitive than the homogeneous-heterogeneous format.
Article Synopsis
- Two types of assays (heterogeneous and homogeneous-heterogeneous) were developed to quantify hsa-miR-141-3p using isothermal circular strand-displacement polymerization (ICSDPR) and enhanced chemiluminescence for improved sensitivity.
- The heterogeneous assay proved to be more sensitive than the homogeneous-heterogeneous assay, with detection limits of 51 fM and 10 pM, respectively, and an amplification index of 100.
- The assays successfully determined the levels of miRNA-141 in various human cell lines, revealing distinct copy numbers per cell (Caco-2: 3400, HepG2: 1400, MCF-7: 1300, HeLa: 470).
A sensitive and specific heterogeneous assay for quantitation of cel-miRNA-39-3p (miRNA-39) was constructed. To improve the assay sensitivity an amplification strategy based on the use of isothermal circular strand-displacement polymerization reaction (ICSDPR), polyperoxidase conjugated with streptavidin and enhanced chemiluminescence was used. The detection limit of the proposed assay was 4 × 10 M.
View Article and Find Full Text PDFSensitive microplate-based chemiluminescent assay coupled with isothermal circular strand-displacement polymerization reaction (ICSDPR) for DNA detection was developed. The assay sensitivity was improved using a triple amplification strategy based on employment of ICSDPR, streptavidin-polyperoxidase conjugate and an enhanced chemiluminescent reaction. To increase the accuracy all stages of the assay (one-pot format) were carried out in the same microplate well.
View Article and Find Full Text PDFBackground: Inguinal hernia repair is the most common procedure in pediatric surgery. Suture techniques for laparoscopic inguinal hernia repair in children are easy to perform and popular with a low recurrence rate. The aim of this study was to evaluate the effect of laparoscopic preperitoneal injection of three-dimensional gel on closing of the inguinal hernia sac (IHS) in laboratory animals.
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