[Development of diagnostic test system based on fluorescent polarization immunoassay method for detection of antibodies to HCV nucleocapsid protein].

The antigen activity of the synthetic fluorescently labeled peptides, overlapping immunoresponsive epitops a.a. 7-19, 20-34 from N-end part and a.

Regulation of NAD(+)-dependent isocitrate dehydrogenase in the citrate producing yeast Yarrowia lipolytica.

The mechanism of the increased accumulation (overproduction) of citric acids in the yeast Yarrowia lipolytica while growing in the presence of glucose under nitrogen deficiency was investigated. The limitation of the yeast growth by the source of nitrogen decreases the total content of nucleotides and increases the ratios of ATP/AMP and NADH/NAD+. NAD+-Dependent isocitrate dehydrogenase, an enzyme of the tricarboxylic acid cycle playing a key role in the regulation of biosynthesis of citric and isocitric acids, was isolated from Y.

[The level of cytochrome P-450 in Saccharomyces cerevisiae with disruptions of various stages of sterol synthesis].

A spectral analysis of cytochromes P-450 in Saccharomyces cerevisiae cells and in mutant strains accumulating the ergosterol biosynthesis intermediates was carried out. Glucose repression and semianaerobiosis were found to induce cytochrome P-450 synthesis. No differences in the cytochrome P-450 content in mutant nys 3, nys 4 and parent strains were observed.

Inhibition of succinate oxidation and K+ transport in mitochondria during hibernation.

Respiration of liver mitochondria of ground squirrels changes with physiological state. The inhibition of respiration at the level of dehydrogenases occurs during hibernation which is spontaneously removed during arousal. The main mechanism causing a decrease in respiration during hibernation seems to be the inhibition of succinate oxidation, induced by oxaloacetic acid.