Feasibility of using an artificially intelligent chatbot to increase access to information and sexual and reproductive health services.

Background: Following the US Supreme Court decision overturning Roe v. Wade, there is evidence of limitations in access to safe abortion care. Artificially intelligent (AI)-enabled conversational chatbots are becoming an appealing option to support access to care, but generative AI systems can misinform and hallucinate and risk reinforcing problematic bias and stigma related to sexual and reproductive healthcare.

An artificially intelligent, natural language processing chatbot designed to promote COVID-19 vaccination: A proof-of-concept pilot study.

Objective: Our goal is to establish the feasibility of using an artificially intelligent chatbot in diverse healthcare settings to promote COVID-19 vaccination.

Methods: We designed an artificially intelligent chatbot deployed via short message services and web-based platforms. Guided by communication theories, we developed persuasive messages to respond to users' COVID-19-related questions and encourage vaccination.

The Xis2d protein of CTnDOT binds to the intergenic region between the mob and tra operons.

CTnDOT is a 65kbp integrative and conjugative element (ICE) that carries genes encoding both tetracycline and erythromycin resistances. The excision operon of this element encodes Xis2c, Xis2d, and Exc proteins involved in the excision of CTnDOT from host chromosomes. These proteins are also required in the complex transcriptional regulation of the divergently transcribed transfer (tra) and mobilization (mob) operons of CTnDOT.

Regulation of CTnDOT conjugative transfer is a complex and highly coordinated series of events.

Unlabelled: CTnDOT is a 65-kb conjugative transposon that is found in Bacteroides spp., which are one of the more abundant members within the lower human gastrointestinal tract. CTnDOT encodes resistance to the antibiotics erythromycin and tetracycline (Tc).

Tetracycline-related transcriptional regulation of the CTnDOT mobilization region.

CTnDOT is a 65-kb conjugative transposon (CTn) in Bacteroides spp. that confers resistance to the antibiotics erythromycin and tetracycline (Tc). Conjugative transfer of CTnDOT is regulated upon exposure of cells to Tc.