[Non-invasive assessment used to evaluate the nasal and oral mucosal cytological status in sociohygienic monitoring].

By analyzing their own studies and the results of other studies by other investigators, the authors provide evidence that the noninvasive evaluation of the nasal and oral cytological status is one of techniques for assessing the health status and reflects the organism's state varying with environmental pollution, which enables it to be recommended for sociohygienic monitoring.

[Reactions of different cell populations of rats to intake of waters treated with physical methods].

A 6-month experiment was carried out to study the reactions of different cell populations of the testicle and duodenum of rats and the gastrointestinal tract of volunteers to the intake of 3 waters treated by physical methods: water conditioned with electric pulsed discharges; 2 waters obtained using energoinformational technologies: Grander water (Austria) and immunomodulated water Renorm (Russia). The test waters treated by physical methods were shown to have no negative effect or a positive effect on animals and man. Three used procedures to evaluate different gastrointestinal tract portions (oral and intestinal mucosae) of rats and volunteers revealed the preservation of the mucosal epithelium when Grander and Renorm waters are taken in doses.

[Use of micronuclear test to assess drinking waters].

The effect of drinking waters with their altered structure and charge state (a total of 9 samples) on the epitheliocytes of the proventriculus, liver, and kidney was studied in rats after 6- and 12-month water consumption. It was found that in the absence of changes in the animals' morphofunctional status, the degree of water structuredness was associated with the frequency of epitheliocytes with nuclear protrusion into the proventricular mucosa following 6-month water consumption and with the manifestations of adipose degeneration, hepatocytic alterations, and stromal overgrowth into the liver after 12-month water use. The biological implication of little studied (additional) types of anomalies of the proventricular epitheliocytic nucleus: the necrotic nature of a perinuclear vacuole, the predominant implication of nuclear pyknosis and rrhexis to apoptosis and the association of binucleated patterns with the restorative proliferation of hepatic and renal tissues, was specified.

[Use of morphofunctional and cytogenetic studies to analyze the influence of environmental factors].

Long-term studies of 2 (cytohistology and genetic monitoring) laboratories of the Research Institute of Human Ecology and Environmental Hygiene, Russian Academy of Medical Sciences, to investigate the influence of environmental factors in the experiments made it possible to develop a noninvasive procedure for evaluating their cytological and cytogenetic impacts on man. A concurrent study of morphofunctional (histological, cytological) and cytogenetic parameters in both experimental and field trials can substantially extend and refine the interpretation of responses to environmental factors, which generates a need for using these parameters in hygienic and epidemiological studies.

[Structural and functional assessment of the body's status after consumption of the waters treated with various technologies].

The use of waters treated by different technologies was studied in animals and volunteers. Rats were used to study the use of the immunomodulated water "Renorm" alone and in combination with ozone-sorption treated Moscow tap water in a dilution of 1:10, that of the bottled water "Grander" (Austria) in combination with ozone-sorption treated Moscow tap water in a dilution of 1: 4 or 1:40; that of the water prepared by the Grander technology through a shower device when, after ozone-sorption purification, Moscow tap water was passed through a Grander shower device at a rate of 1 liter per min or 500 ml per 30 sec; that of the water electrochemically treated by an Izumrud (Emerald) installation. Moscow tap water served as a control for all waters and was Moscow dechlorinated water after ozone-sorption purification, by passing Moscow tap water from a tap into a 3-liter vessel through a household ozone device for 5 min and then passing it through a household coal filter (500 ml).