Investigation of changes in NO content during long-term sensitization in edible snail using EPR-spectroscopy: effects of antibodies to calcium-binding protein S-100.

EPR-spectroscopy experiments (electron paramagnetic resonance) demonstrated a decrease in NO production in the nervous system and heart of edible snail Helix lucorum after formation of long-term sensitization, a neurobiological model of anxiety and depression. The protective effect of antibodies to Ca(2+)-binding protein S-100 in dilution of 10(-12) on the formation of long-term sensitization was accompanied by partial recovery of NO synthesis in the nervous system and heart. These findings indicate that the imbalance in Ca(2+)-binding protein S-100 can lead to inhibition or modulation of some processes during plastic reorganization in the body and especially during pathological processes.

Electron paramagnetic resonance study of nitric oxide production during 5-HT2 receptor blockade in the blood, heart, and liver of rats with myocardial infarction.

The effect of serotonin 5-HT2 receptor blockade on nitric oxide production in rats with myocardial infarction was evaluated by the method of electron paramagnetic resonance. The spectra were recorded in samples of the heart tissue (left and right chambers), liver, and blood. 5-HT2 receptor blockade during myocardial infarction was followed by a decrease in the total content of nitrosyl complexes in the spin trap and R and T conformers of Hb-NO.