[The effect of the delta-sleep peptide on the adrenaline level in rat tissues under normal conditions and during cold stress].

Adrenalin content in the brain, liver and adrenal glands under the effect of cold stress grows by 314, 500 and 56% as compared to the control. A single administration of the delta-sleep inducing peptide (DSIP) in a dose of 12 microgram/100 g to intact animals makes the adrenalin content in the brain higher 1, 3, 6 and 24h after administration; two and three days later the adrenalin content in the brain does not change. The amount of adrenalin in the liver of the same animals increases 1, 3, 6 h and 1, 2, 3 days after DSIP administration.

[Interaction of products of su(Hw) and su(f) genes with MDG4 region regulating its transcription suppresses mutations in Drosophila caused by insertion of this gene].

The mdg4 (gypsy) mobile element of Drosophila contains two closely situated regions binding to proteins from nuclear extracts. One of these is an imperfect palindrome having homology with the lac operator of Escherichia coli, the other contains a reiterated sequence (5'PyPuTCTGCATACTPyPy) homologous to the octamer which is the core of many enhancers and upstream promoter elements. The transient expression of deletion mutants has shown that these DNA regions are negative and positive regulators, respectively, of mdg4 transcription.

[The Drosophila mobile element jockey, being a typical LINE, is transcribed from the internal promoter by RNA polymerase II].

Two polyadenylated transcripts of the jockey are detected at different stages of Drosophila melanogaster ontogenesis and in the cell culture. They have the same length as complete and deleted copies of jockey and correspond to the DNA strand containing open reading frames coding for polypeptides which are homologous to retroviral RNA-(DNA)-binding proteins and to their reverse transcriptases. The results of the experiments, where transcription was inhibited with alpha-amanitin in vivo, indicate that jockey is transcribed by RNA polymerase II.