[Effect of inert gas xenon on the functional state of nucleated cells of peripheral blood during freezing].

A new method of preservation of nucleated cells in the electric refrigerator with xenon. After slow freezing and storage is even one day at -80 °C persists for more than 60% leukocytes. Cell membranes are resistant to the vital dye.

Influence of pectins on NADPH oxidase and phagocytic activity of neutrophils during cryopreservation.

NADPH oxidase activation was studied by detecting the reactive oxygen species (ROS) and the dynamics of neutrophil phagocytic activity in blood before freezing and one day after exposure to -20 degrees C in the presence of pectic polysaccharides. Pectins (excluding lemnan) were found to increase the phagocytic activity of neutrophils, promote the formation of ROS in these cells and enhance NADPH oxidase activity in the following order: bergenan, comaruman, lemnan, apiuman, and potamogetonan. Cryoprotective action of lemnan and comaruman was proposed to be related to their unique chemical structures.

Combined effects of blood serum as a source of endogenous β-adrenoceptor-sensitizing agent and its analogues histidine, tryptophan, tyrosine, mildronat, and preductal.

Human blood serum exhibiting β-adrenoceptor-sensitizing activity does not prevent manifestation of similar activity of histidine, tryptophan, tyrosine, mildronat, and preductal. This opens prospects for the use of analogues of endogenous β-adrenoceptor-sensitizing agent in clinical practice.

Phagocytic activity of neutrophils and lymphocytes at various periods of storage in the state of cold anabiosis (-40 degrees C).

We studied changes in phagocytic activity of neutrophils and lymphocytes subjected to cold anabiosis. The proposed effective method for introduction of human blood leukocytes into cold anabiosis at moderately low temperature (-40 degrees C) in the presence of a cryopreserving agent does not require washout of the biological material after defrosting.

A method for introducing human blood leukocytes in cold anabiosis at -50 degrees Celsius.

We propose a simple, effective, and economic method for introducing human blood leukocytes in cold anabiosis at moderately low temperature (-50 degrees Celsius).