Publications by authors named "A A Kavaleuski"
The potential of immune-evasive mutation accumulation in the SARS-CoV-2 virus has led to its rapid spread, causing over 600 million confirmed cases and more than 6.5 million confirmed deaths. The huge demand for the rapid development and deployment of low-cost and effective vaccines against emerging variants has renewed interest in DNA vaccine technology.
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- Ferredoxins are small proteins that help with important processes in living things, but a specific type called 3Fe-4S ferredoxins hasn't been studied much because they need special conditions to exist.
- Scientists figured out the structure of two types of these ferredoxins, showing how they work with another protein called CYP143.
- The study found that when FdxE connects to CYP143, it changes its shape a bit, which helps in making different kinds of important chemicals.
The COVID-19 pandemic not only resulted in a global crisis, but also accelerated vaccine development and antibody discovery. Herein we report a synthetic humanized VHH library development pipeline for nanomolar-range affinity VHH binders to SARS-CoV-2 variants of concern (VoC) receptor binding domains (RBD) isolation. Trinucleotide-based randomization of CDRs by Kunkel mutagenesis with the subsequent rolling-cycle amplification resulted in more than 10 diverse phage display library in a manageable for a single person number of electroporation reactions.
View Article and Find Full Text PDFNanobodies (VHH) from camelid antibody libraries hold great promise as therapeutic agents and components of immunoassay systems. Synthetic antibody libraries that could be designed and generated once and for various applications could yield binders to virtually any targets, even for non-immunogenic or toxic ones, in a short term. One of the most difficult tasks is to obtain antibodies with a high affinity and specificity to polyglycosylated proteins.
View Article and Find Full Text PDFElectron transfer mediated by metalloproteins drives many biological processes. Rubredoxins are a ubiquitous [1Fe-0S] class of electron carriers that play an important role in bacterial adaptation to changing environmental conditions. In Mycobacterium tuberculosis, oxidative and acidic stresses as well as iron starvation induce rubredoxins expression.
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