Characterization, structural, and evolutionary analysis of an extremophilic GH5 endoglucanase from Bacillus sp. G131: Insights from ancestral sequence reconstruction.

Nature has developed extremozymes that catalyze complex reaction processes in extreme environmental conditions. Accordingly, a combined approach consisting of extremozyme screening, ancestral sequence resurrection (ASR), and molecular dynamic simulation was utilized to construct a developed endoglucanase. The primary experimental and in-silico data led to the prediction of a hypothetical sequence of endoglucanase (EG5-G131) using Bacillus sp.

Introns and Their Therapeutic Applications in Biomedical Researches.

Context: Although for a long time, it was thought that intervening sequences (introns) were junk DNA without any function, their critical roles and the underlying molecular mechanisms in genome regulation have only recently come to light. Introns not only carry information for splicing, but they also play many supportive roles in gene regulation at different levels. They are supposed to function as useful tools in various biological processes, particularly in the diagnosis and treatment of diseases.

Icariin: A Promising Natural Product in Biomedicine and Tissue Engineering.

Among scaffolds used in tissue engineering, natural biomaterials such as plant-based materials show a crucial role in cellular function due to their biocompatibility and chemical indicators. Because of environmentally friendly behavior and safety, green methods are so important in designing scaffolds. A key bioactive flavonoid of the plant, Icariin (ICRN), has a broad range of applications in improving scaffolds as a constant and non-immunogenic material, and in stimulating the cell growth, differentiation of chondrocytes as well as differentiation of embryonic stem cells towards cardiomyocytes.

Heavy metal analysis in of indoor and outdoor dust extracts and cytotoxicity evaluation and inflammation factors on lung, gastric and skin cell lines.

Dust particles (DPs) are one of the most important public health concerns in the urban environment. The presence of heavy metals (HMs) on the surface of DPs might increase the health risk of exposure to the DPs. Accordingly, The purpose of this study was to examine the content of HMs in the outdoor and indoor DPs in Neyshabur city and assess the cytotoxic effects of DPs exposure on lung, gastric, and skin cell lines.