Publications by authors named "A A Franken"
Article Synopsis
- Kidney structural cells actively participate in the rejection process of kidney transplants, alongside infiltrated immune cells, as shown through single-cell RNA sequencing of biopsies.
- Upregulation of immune-related genes was observed in endothelial and epithelial cells, indicating their involvement in the alloimmune response, with distinct differences based on the kidney compartment.
- A significant downregulation of energy metabolism in proximal tubular cells during inflammation correlates with reduced kidney function, emphasizing the graft's crucial role in driving and maintaining rejection after transplantation.
The spatial organization of cells within tissues aids in understanding physiological and pathological processes, as well as elucidating the mechanisms of action underlying treatments. We present a protocol for analyzing image-based spatial proteomics data. To illustrate, we focus on whole-slide images of human multiplexed tumor tissues acquired using the PhenoCycler-Fusion 2.
View Article and Find Full Text PDFArticle Synopsis
- Circulating tumor cells and liquid biopsy markers could significantly influence cancer treatment decisions in the future.
- This study compares two reverse transcription enzymes, SuperScript IV VILO and Sensiscript, focusing on their effectiveness in detecting biomarkers in prostate cancer cell lines and clinical liquid biopsy samples.
- Results show that VILO outperforms SS, leading to more patients being correctly identified as biomarker-positive, which is crucial for effective clinical monitoring and treatment decisions.
Article Synopsis
- Traditional genomic profiling of Circulating Tumor Cells (CTCs) misses important protein alterations affecting treatment efficacy, leading to the development of the ZeptoCTC workflow, which analyzes single cells at the protein level.
- The ZeptoCTC process involves isolating and labeling individual cells, lysing them, and using reverse phase protein array (RPPA) detection for precise protein quantification.
- Results showed ZeptoCTC's effectiveness by revealing significant protein expression differences in CTCs from breast cancer patients and its ability to differentiate based on genetic variants, enhancing understanding of tumor behavior.
For youth care professionals who work with families with complex needs, we implemented an interagency, family-focused approach involving child and adult mental health care services and child protection services. The primary objective of the collaboration was to minimize fragmentation in service delivery and to improve practitioners' self-efficacy in supporting families. A total of 50 families were enrolled between 2020 and 2023.
View Article and Find Full Text PDF