[SEPARATE LEGAL AND MEDICAL AND SOCIAL ASPECTS OF POSTTHEAL TRANSPLANTATION IN UKRAINE].

Organ donation is one of the topical issues in the field of medicine around the world. Although the practice of transplantation exists in most countries of the world, there are still many unresolved issues. One of them is the issues related to postmortem organ transplantation in Ukraine.

NF-Ya protein delivery as a tool for hematopoietic progenitor cell expansion.

The clinical potential of therapeutic quantities of primary hematopoietic cells, either unmodified or altered via genetic modification, has stimulated the search for techniques that allow the production of large numbers of hematopoietic precursors, more primitive progenitors, and perhaps hematopoietic stem cells (HSC) themselves. Modifications of in vitro culture conditions to promote progenitor cell expansion have included combinations of polypeptide cytokines, small molecules, and transcription factors. Here we describe the methods for use of the transcription factor linked to a TAT-based protein transcription domain, in combination with cytokines and serum-free culture condition to stimulate the proliferation of primary cells.

[The method of replacement of defects in impression fractures of calcaneus. Porous NiTi or autotransplant?].

An experience with treatment of 149 patients with severe injuries of the ankle joint operated using constructions of titanium-nickelide and autotransplant from the iliac crest is presented. Porous NiTi being bio-inert to organism tissues, having high through porosity, the formation of consolidation of the fracture develops more rapidly. Application of porous NiTi allowed the period of disablement to be on an average 20 days shorter and long-term results to be reliably better.

TAT-mediated transduction of NF-Ya peptide induces the ex vivo proliferation and engraftment potential of human hematopoietic progenitor cells.

Retroviral overexpression of NF-Ya, the regulatory subunit of the transcription factor NF-Y, activates the transcription of multiple genes implicated in hematopoietic stem cell (HSC) self-renewal and differentiation and directs HSCs toward self-renewal. We asked whether TAT-NF-Ya fusion protein could be used to transduce human CD34(+) cells as a safer, more regulated alternative approach to gene therapy. Here we show that externally added recombinant protein was able to enter the cell nucleus and activate HOXB4, a target gene of NF-Ya, using real-time polymerase chain reaction RNA and luciferase-based protein assays.