Publications by authors named "A A Belogurov"
Lymphotoxin α and lymphotoxin β (LTs), TNF superfamily members, are expressed in either soluble (LTα) or membrane-bound (LTαβ or LTαβ) forms. In the pathological context, LT-mediated signaling is known to exacerbate autoimmunity by perpetuating inflammation and promoting the formation of tertiary lymphoid organs. Despite this understanding, the exact roles of LTα and LTβ in the pathogenesis of the murine model of multiple sclerosis, and experimental autoimmune encephalomyelitis (EAE), remain controversial.
View Article and Find Full Text PDFUbiquitination is an evolutionary, ancient system of post-translational modification of proteins that occurs through a cascade involving ubiquitin activation, transfer, and conjugation. The maturation of this system has followed two main pathways. The first is the conservation of a universal structural fold of ubiquitin and ubiquitin-like proteins, which are present in both Archaea and Bacteria, as well as in multicellular Eukaryotes.
View Article and Find Full Text PDFIn this study, we evaluated the ability of the synthetic amphipathic helical peptide (SAHP), L-37pA, which mediates pathogen recognition and innate immune responses, to treat acute respiratory distress syndrome (ARDS) accompanied by diffuse alveolar damage (DAD) and chronic pulmonary fibrosis (PF). For the modeling of ARDS/DAD, male ICR mice were used. Intrabronchial instillation (IB) of 200 µL of inflammatory agents was performed by an intravenous catheter 20 G into the left lung lobe only, leaving the right lobe unaffected.
View Article and Find Full Text PDFIncautious design of shRNAs for stable overexpression of miRNAs could result in generation of undesired isomiRs.
Biochim Biophys Acta Gene Regul Mech
September 2024
Article Synopsis
- A study highlights that using shRNA-based methods for miRNA overexpression, especially for certain miRNAs from polycistronic clusters, can lead to unintentional creation of 5' end isoforms (5'-isomiRs) that differ from the intended miRNA, affecting research accuracy.
- The transcription process of shRNAs via RNA Polymerase III can add extra nucleotides, altering the cleavage site and resulting in 5'-isomiRs with changed seed regions, which impacts their function.
- Standard qPCR methods fail to detect these alterations, highlighting the need for miRNA-Seq analysis to accurately assess miRNA expression and their regulation of mRNA targets, emphasizing careful design for shRNA constructs.
Aim: To evaluate the levels of MPO-DNA complex in patients with systemic lupus erythematosus (SLE) and its association with the presence of lupus nephritis (LN).
Materials And Methods: The study included 77 patients with SLE, of whom 30 had SLE without anti phospholipid syndrome (APS), 47 had SLE with APS, and 20 were healthy individuals serving as the control group. The MPO-DNA complex in the serum was investigated using ELISA.