[Interaction between cytochrome P-450scc and adrenodoxin in solution under effects of low spin effectors].

The interaction between cytochrome P-450scc and adrenodoxin and cholesterol under effects of low spin effectors (detergents, pregnolone) was studied. The increase in Tween 20 concentration causes a rise in the Kd values for cytochrome P-450scc complexes with adrenodoxin and cholesterol. Both cholesterol and adrenodoxin can convert the low spin form of cytochrome P-450scc obtained by addition of Tween 20 to the high spin form; however, their combined action results in higher values of the spin state index (A393/A418) at final titration points.