Construction and validation of a regulatory T cells-based classification of renal cell carcinoma: an integrated bioinformatic analysis and clinical cohort study.

Purpose: Renal cell carcinoma (RCC), exhibiting remarkable heterogeneity, can be highly infiltrated by regulatory T cells (Tregs). However, the relationship between Treg and the heterogeneity of RCC remains to be explored.

Methods: We acquired single-cell RNA-seq profiles and 537 bulk RNA-seq profiles of TCGA-KIRC cohort.

Transurethral resection of bladder tumor with Bacille Calmette-Guerin intravesical instillation for HRNMIBC and factors influencing recurrence.

Objective: To investigate the effectiveness of combining transurethral resection of bladder tumor (TURBT) with Bacille Calmette-Guerin (BCG) intravesical instillation in treating high-risk non-muscle invasive bladder cancer (HRNMIBC).

Methods: We retrospectively reviewed the clinical data from 118 HRNMIBC patients treated at Tongren Hospital between March 2020 and June 2022. The patients were categorized into two groups based on their treatment regimen: the control group (n=60) which received regular pirarubicin intravesical instillation, and the observation group (n=58) which received additional BCG intravesical instillation alongside pirarubicin.

The therapeutic effects of theta burst stimulation on negative symptoms in chronic schizophrenia using functional near-infrared spectroscopy.

Background: To explore the efficacy of theta burst stimulation (TBS) on the negative symptoms in patients with chronic schizophrenia, and to investigate the alterations of local brain activity using functional near-infrared spectroscopy (fNIRS).

Methods: 100 patients with chronic schizophrenia were enrolled and divided into the real group (50 subjects) and sham group (50 subjects). The real group was given real stimulation of TBS for 4 weeks, and the sham group was sham-stimulated with the same site.

Antifungal activity of bamemacrolactine C against Talaromyces marneffei and its possible mechanisms of action.

Aims: The present study aims to investigate the in vitro antifungal activity and mechanism of action of bamemacrolactine C (BAC), a new 24-membered macrolide compound, against Talaromyces marneffei.

Methods And Results: The test drug BAC initially demonstrated antifungal activity through a paper disk diffusion assay, followed by determination of the minimum inhibitory concentration value of 35.29 μg ml-1 using microdilution.

Preparation and cell imaging of a nido-carborane fluorescent complex based on multi-component polymerization.

The biocompatibility of carborane was a difficult problem that had drawn a lot of study interest. Using multi-ion inlay binding, water-soluble polymers were created by encapsulating nido-carborane in diazaspirodecaniums such as para-poly-nido-carboanylazaspirodecanium [5,4] (p-PNC54), para-poly-nido-carboanylazaspirodecanium [6,5] (p-PNC65), meta-poly-nido-carboanylazaspirodecanium [5,4] (m-PNC54), and meta-poly-nido-carboanylazaspirodecanium [6,5] (m-PNC65). First, the active control 5-fluorouracil demonstrated strong activity against HeLa and HCT-116 cells but minimal cytotoxicity at 19.

Androgen receptor inhibitors in treating prostate cancer.

Androgens play an important role in prostate cancer development and progression. Androgen action is mediated through the androgen receptor (AR), a ligand-dependent DNA-binding transcription factor. AR is arguably the most important target for prostate cancer treatment.

Plant growth-promoting fungi improve tobacco yield and chemical components by reassembling rhizosphere fungal microbiome and recruiting probiotic taxa.

Background: Tobacco production faces ongoing challenges due to soil degradation, leading to a persistent decline in yield. Plant growth-promoting fungi (PGPF) have been recognized as an environmentally friendly agricultural strategy. However, many commercial PGPF products exhibit instability due to insufficient environmental compatibility.

Multimodal Ultrasound Radiomic Technology for Diagnosing Benign and Malignant Thyroid Nodules of Ti-Rads 4-5: A Multicenter Study.

This study included 468 patients and aimed to use multimodal ultrasound radiomic technology to predict the malignancy of TI-RADS 4-5 thyroid nodules. First, radiomic features are extracted from conventional two-dimensional ultrasound (transverse ultrasound and longitudinal ultrasound), strain elastography (SE), and shear-wave-imaging (SWE) images. Next, the least absolute shrinkage and selection operator (LASSO) is used to screen out features related to malignant tumors.

The association between sleep disorder, sleep duration and chronic back pain: results from National Health and Nutrition Examination Surveys, 2009-2010.

Background: Back pain is a typical condition, and the association among sleep disorders, sleep duration and back pain is currently being investigated. The purpose of this research is to explore the connection between sleep disorders, sleep duration and chronic back pain as well as confounding factors.

Methods: Our data were obtained from the National Health and Nutrition Examination Survey (NHANES) data set of the USA and 1,131 participants were included in the study.

Adsorption of Congo red on magnetic cobalt-manganese ferrite nanoparticles: Adsorption kinetic, isotherm, thermodynamics, and electrochemistry.

Magnetic Co0.5Mn0.5Fe2O4 nanoparticles were successfully prepared via the combustion and calcination process, with an average particle diameter of 31.

High-capacity, fast-charging and long-life magnesium/black phosphorous composite negative electrode for non-aqueous magnesium battery.

Secondary non-aqueous magnesium-based batteries are a promising candidate for post-lithium-ion battery technologies. However, the uneven Mg plating behavior at the negative electrode leads to high overpotential and short cycle life. Here, to circumvent these issues, we report the preparation of a magnesium/black phosphorus (Mg@BP) composite and its use as a negative electrode for non-aqueous magnesium-based batteries.

Neural Memory State Space Models for Medical Image Segmentation.

With the rapid advancement of deep learning, computer-aided diagnosis and treatment have become crucial in medicine. UNet is a widely used architecture for medical image segmentation, and various methods for improving UNet have been extensively explored. One popular approach is incorporating transformers, though their quadratic computational complexity poses challenges.

Sub-THz High Spin Precession Frequency in van der Waals Ferromagnet FeGaTe.

The 2D magnet FeGaTe has received considerable attention for its high Curie temperature (), robust intrinsic ferromagnetism, and significant perpendicular magnetic anisotropy (PMA). In this study, the dynamic magnetic properties of FeGaTe are systematically investigated using an all-optical pump-probe technique. We find that the spin precession frequency () is as high as 351.

TRIB3 knockdown increases the sensitivity of clear cell renal cell carcinoma to sunitinib by inducing ferroptosis.

Sunitinib resistance presents a significant challenge in the treatment of clear cell renal cell carcinoma (ccRCC). The role of TRIB3, a newly identified oncogene, in tumor drug resistance has been widely studied. However, the mechanism by which TRIB3 contributes to sunitinib resistance in ccRCC has not been previously explored.

Repurposing Niclosamide to Modulate Renal RNA-Binding Protein HuR for the Treatment of Diabetic Nephropathy in db/db Mice.

Hu antigen R (HuR) plays a key role in regulating genes critical to the pathogenesis of diabetic nephropathy (DN). This study investigates the therapeutic potential of niclosamide (NCS) as an HuR inhibitor in DN. Uninephrectomized mice were assigned to four groups: normal control; untreated db/db mice terminated at 14 and 22 weeks, respectively; and db/db mice treated with NCS (20 mg/kg daily via i.

Interpretable Machine Learning Model Based on Superb Microvascular Imaging for Non-Invasive Determination of Crescent Status of IgAN.

Purpose: To assess the crescentic status of IgA nephropathy (IgAN) non-invasively using a superb microvascular imaging (SMI)-based radiomics machine learning (ML) model.

Patients And Methods: IgAN patients who underwent renal biopsy from June 2022 to October 2023, with two-dimensional ultrasound (US) and SMI examinations conducted one day prior to the renal biopsy. The patients selected were divided randomly into a training group and a test group in a 7:3 ratio.

KLF15 ATTENUATES LIPOPOLYSACCHARIDE-INDUCED APOPTOSIS AND INFLAMMATORY RESPONSE IN RENAL TUBULAR EPITHELIAL CELLS VIA PPARΔ.

Background: The kidney is the most commonly affected organ in sepsis patients, and Krüppel-like transcription factor 15 (KLF15) has a kidney-protective effect and is highly enriched in the kidneys. This study aims to explore the role of KLF15 in sepsis-related acute kidney injury. Methods: A septic injury model in HK2 cells was established through the administration of lipopolysaccharide (LPS), followed by the transfection of an overexpression plasmid for KLF15.

Discovery and preclinical profile of YK-2168, a differentiated selective CDK9 inhibitor in clinical development.

Emerging clinical evidence indicates that selective CDK9 inhibition may provide clinical benefits in the management of certain cancers. Many CDK9 selective inhibitors have entered clinical developments, and are being investigated. No clear winner has emerged because of unforeseen toxicity often observed in clinic with these agents.

Biochemical Properties of a Novel Cold-Adapted GH19 Chitinase with Three Chitin-Binding Domains from CSC-1 and Its Potential in Biocontrol of Plant Pathogenic Fungi.

GH19 (glycoside hydrolase 19) chitinases play crucial roles in the enzymatic conversion of chitin and biocontrol of phytopathogenic fungi. Herein, a novel multifunctional chitinase of GH19 (Chi19A), which contains three chitin-binding domains (ChBDs), was successfully cloned from CSC-1 and heterologously expressed in . We also generated truncated mutants of Chi19A_ΔI, Chi19A_ΔIΔII, and Chi19A_CatD consisting of two ChBDs and a catalytic domain, one ChBD and a catalytic domain, and only a catalytic domain, respectively.