Two new species of Nectriaceae (Hypocreales, Sordariomycetes) from Yunnan, China.

Nectriaceae is a highly diverse family, and members have a worldwide distribution, particularly in warm temperate to tropical regions. During the survey of fungal diversity in different habitats in Yunnan province, China, two new species isolated from soil and air respectively, namely and , were proposed based on morphological comparisons and the multi-gene phylogenetic analyses of combined ITS, LSU, 2, and 2 sequence data. Phylogenetically, both species clustered in a monophyletic clade within Nectriaceae with strong support.

Diversity of species associated with the black rot disease of , including four new species.

Introduction: species establish symbiotic relationships with plants through both parasitic and mutualistic mechanisms. While some species act as plant pathogenic fungi, others utilize various strategies to protect and enhance plant growth.

Methods: Phylogenetic positions of new species of were determined through multi-gene analysis relying on the internal transcribed spacer (ITS) regions of the ribosomal DNA, the translation elongation factor 1-α (α) gene, and the RNA polymerase II () gene.

DNA methylation plays important roles in lifestyle transition of Arthrobotrys oligospora.

Trap formation is the key indicator of carnivorous lifestyle transition of nematode-trapping fungi (NTF). Here, the DNA methylation profile was explored during trap induction of Arthrobotrys oligospora, a typical NTF that captures nematodes by developing adhesive networks. Whole-genome bisulfite sequencing identified 871 methylation sites and 1979 differentially methylated regions (DMRs).

Endosymbiotic bacteria within the nematode-trapping fungus and their potential roles in nitrogen cycling.

Endosymbiotic bacteria (ESB) have important effects on their hosts, contributing to its growth, reproduction and biological functions. Although the effects of exogenous bacteria on the trap formation of nematode-trapping fungi (NTF) have been revealed, the effects of ESB on NTF remain unknown. In this study, we investigated the species diversity of ESB in the NTF using high-throughput sequencing and culture-dependent approaches, and compared bacterial profiles to assess the effects of strain source and culture media on .

Two new species (Hypocreales, Hypocreaceae) isolated from decaying tubers of .

Species of are widely distributed around the world. In this study, two new species in , named as and , were introduced and illustrated. These species were isolated from diseased tubers of in China and identified based on morphological characteristics and multi-gene sequence analyses of three loci that is the internal transcribed spacer regions of the ribosomal DNA (ITS), the translation elongation factor 1-α encoding gene () and the gene encoding the second largest nuclear RNA polymerase subunit ().

Analyses of Multilocus Sequences and Morphological Features Reveal Species Associated with Black Rot Disease of .

Black rot is a common disease of , causing serious threats to production. In this study, a total of 17 -like strains were isolated from black rot tissues. Multilocus sequence analyses based on ITS, HIS, TEF, and TUB combined with morphological characterizations were performed to identify six species, including four new species, , , , and , and two known species, and The pathogenicity of 11 isolates comprising type strains of the four new species and representative isolates from each of the six species was tested on healthy tissues of .

New Species of within Aquatic Plants from Southwestern China.

Members of have a wide geographical distribution throughout different ecosystems, and most species are associated with fruit, leaf, stem and root diseases of land plants. However, species that occur in aquatic plants are not clearly known. During a survey of the diversity of endophytes in aquatic plants in Yunnan, Sichuan, and Guizhou provinces, we obtained 51 isolates belonging to based on internal transcribed spacer region (ITS) sequences.

Aspergillus niger produces lethal compounds against nematodes.

Background: Aspergillus niger is generally considered safe and is widely used across a broad range of industries from food to pharmaceuticals. The metabolites of some Aspergillus species are effective in killing nematodes. The active ingredients that play a role are citric acid and oxalic acid.

Identification of and Mechanistic Insights into SARS-CoV-2 Main Protease Non-Covalent Inhibitors: An In-Silico Study.

The indispensable role of the SARS-CoV-2 main protease (Mpro) in the viral replication cycle and its dissimilarity to human proteases make Mpro a promising drug target. In order to identify the non-covalent Mpro inhibitors, we performed a comprehensive study using a combined computational strategy. We first screened the ZINC purchasable compound database using the pharmacophore model generated from the reference crystal structure of Mpro complexed with the inhibitor ML188.

 and (Chaetothyriales, Herpotrichiellaceae), two new species of soil-inhabiting from Yunnan Province, China.

During a survey of soil fungi collected from Yunnan Province, China, two new species of , and , were isolated from the soil of karst rocky desertification (KRD). The DNA sequences of these respective strains, including internal transcribed spacers (ITS), large subunit nuclear ribosomal RNA (LSU rRNA), partial small subunit (SSU) and β-tubulin () were sequenced and compared with those from species closely-related to . differs from the phylogenetically closely related and by its smaller aseptate conidia.

Cordana yunnanensis sp. nov., Isolated from Desertified Rocky Soil in Southwest China.

During a survey of soil fungal diversity in a rocky desertification area in Yunnan Province, China, a new dematiaceous hyphomycete, Cordana yunnanensis was identified. Morphologically, this species is characterized by macronematous, mononematous conidiophores with discrete, polyblastic conidiogenous cells arranged in a whorl at the apices. Phylogenetic analysis of the combined sequences of the internal transcribed spacer and the large nuclear ribosomal RNA subunit confirmed the phylogenetic position of C.

Colletotrichum litangense sp. nov., Isolated as an Endophyte of Hippuris vulgaris, an Aquatic Plant in Sichuan, China.

An unknown endophytic fungus was isolated from the aquatic plant Hippuris vulgaris in Litang county, Sichuan province, China. Phylogenetic analyses inferred from combined ITS, Sod2, Apn2, and TUB2 sequences revealed that the endophyte is a new species belonging to the Colletotrichum graminicola species complex. Morphological characteristics showed that Colletotrichum litangense is characterized by its falcate, lunate to sublunate conidia, and ellipsoidal, ovoid, or lobed appressoria.

Fourteen New Species of Foliar Associated with the Invasive Plant and Surrounding Crops.

is one of the most invasive weeds in China. Following an outbreak in Yunnan in the 1960s, has been spreading in Southwest China at tremendous speed. Previous research indicated contained many species as endophytes.

Endophytic Species from Aquatic Plants in Southwest China.

species are plant pathogens, saprobes, and endophytes in many economically important hosts. Many studies have investigated the diversity and pathogenicity of species in common ornamentals, fruits, and vegetables. However, species occurring in aquatic plants are not well known.

Keqinzhangia aquatica gen. et sp. nov. and Pseudocoronospora hainanense gen. et sp. nov., isolated from freshwater in southern China.

During an investigation of the diversity of aquatic hyphomycetes from southern China, two interesting isolates were collected. These two isolates were cultured and sequenced, and a BLAST search of their LSU sequences against data in GenBank revealed that the closest related taxa were in the genus Microthyrium. Phylogenetic analyses, based on the combined sequence data from the internal transcribed spacer (ITS) and large nuclear subunit ribosomal DNA (LSU), revealed that our isolates belong to the Microthyriaceae.

Two new asexual genera and six new asexual species in the family Microthyriaceae (Dothideomycetes, Ascomycota) from China.

The family Microthyriaceae is represented by relatively few mycelial cultures and DNA sequences; as a result, the taxonomy and classification of this group of organisms remain poorly understood. During the investigation of the diversity of aquatic hyphomycetes from southern China, several isolates were collected. These isolates were cultured and sequenced and a BLAST search of its LSU sequences against data in GenBank revealed that the closest related taxa are in the genus .

sp. nov., a novel black yeast from soil in southern China.

is an important genus, with several species associated with infections in humans and animals. In a survey of soil fungal diversity in Yunnan province, PR China, a novel taxon, sp. nov.

Culture-Based and Culture-Independent Assessments of Endophytic Fungal Diversity in Aquatic Plants in Southwest China.

Aquatic ecosystems contain tremendous plant and microbial diversity. However, little is known about endophyte diversity in aquatic plants. In this study, we investigated the diversity of endophytic fungi in aquatic plants in southwest China using both culture-based and culture-independent high-throughput sequencing methods.

New Species of Isolated as Endophytes and Saprobes from Southwest China.

During the investigation of endophytic fungi diversity in aquatic plants and the fungal diversity in soil in southwest China, we obtained 208 isolates belonging to , including 28 isolates as endophytes from aquatic plants and 180 isolates as saprobes from soil, respectively. Finally, 23 new species of are recognized by further studies. Their phylogenetic positions are determined by sequence analyses of the combined partial sequences of translation elongation factor 1-alpha (1) and gene encoding of the second largest nuclear RNA polymerase subunit (2).

Diversity of within aquatic plants from southwest China, with and spp. nov.

Members of inhabit different substrates, including plants, soil and insects, and most species are pathogens causing large losses in agriculture. During a survey of endophytic fungi in aquatic plants in southwest China, 112 strains of were isolated, representing two new species, and , as well as two known species, and . The novel taxa are described and illustrated here using combined morphological and multi-locus phylogenetic (LSU-ITS-TEF-1α-TUB2) analyses.

sp. nov., a new hyphomycete (Chaetomiaceae) from China.

, a new hyphomycete classified in the Chaetomiaceae (Ascomycota), was isolated from soil in Jianshui county, Yunnan Province. It is characterized by globose and ochreous conidia born laterally on aerial hyphae, and micronematous, unbranched and 0-1-septate conidiophores, sometimes reduced to conidiogenous cells. Morphologically, is similar to , but it can be distinguished by lacking of macronematous conidiophores and having larger conidia.

sp. nov. (, Chaetosphaeriales), a new species of freshwater hyphomycetes from southwest China.

During a mycological survey of freshwater hyphomycetes on submerged leaves in southwest China, a new species, , was isolated. The new species is characterized by having monophialidic conidiogenous cells with funnel shaped collarettes and globose or subglobose, aseptate and hyaline conidia. Morphologically, is somewhat similar to and in conidiophores and conidiogenous cells, but can be easily distinguished by its subglobose to globose and smaller conidia.

(Chaetosphaeriaceae, Sordariomycetes), a novel asexual genus and species from freshwater in southern China.

The asexual taxon , collected from submerged dicotyledonous leaves in Hainan, China, is described and illustrated herein. is characterized by macronematous, semimacronematous, septate and pigmented conidiophores and acrogenous, long lunate, vermiform to sigmoid, hyaline conidia with an eccentric basal appendage. Complete sequences of internal transcribed spacer (ITS) and partial sequences of nuclear large subunits ribosomal DNA (LSU) genes are provided.

sp. nov. and sp. nov., two novel species isolated from the invasive weed in PR China.

To determine if can accumulate diverse pathogens from surrounding native plants, we intensively sampled fungal communities, including endophytes, leaf spot pathogens and canopy air fungi, associated with as well as native plants in its invasive range. In total, we collected 4542 foliar fungal strains from 10 geographic sites, including 1340 from healthy leaves of , 2051 from leaf spots of and 1151 from leaf spots of 56 species of native plants and crops. Taxonomically, the common fungal genera included , , , , , , , , , , , and .