Analysis of influenza A virus neuraminidase using lectin test and monoclonal antibodies.

Influenza viruses causing epidemics in the U.S.S.

Detection of influenza virus by means of immune sera to M and RNP inner proteins in solid phase radioimmunoassay.

Solid phase radioimmunoassay (SPRIA) was shown suitable for detection of influenza virus in various biological materials using immune sera (IS) to membrane protein (MP) and ribonucleoprotein (RNP) isolated from the strain A/USSR/90/77. Either serum was able to detect influenza A viruses with different haemagglutinins (H1, H3, H7) and did not react with influenza B viruses. SPRIA detected 0.

Differentiation of influenza A virus nucleoproteins in enzyme-linked immunosorbent assay.

The serum antibody titre to the nucleoprotein (NP) of the influenza virus recombinant MRC-11 was determined in virus strains A/USSA/5/80 (H3N2), A/Hong Kong/8/64 (H3N2), A/duck/Ukraine/63 (Hav7Neq2) and in a recombinant strain between A/tern/Frunse/334/78(Hav4Nav1) and A/PR/8/34(H0N1) using the enzyme-linked immunosorbent assay (ELISA). Significant differences between the NP of these strains were found proving the usefulness for ELISA for such investigations.

Gene homology and antigenic specificity of envelope proteins of avian influenza virus strains possessing haemagglutinin Havl.

Comparison of some avian influenza virus strains possessing haemagglutinin Havl revealed the greatest differences in strains A/FPV/Weybridge and A/FPV/Rostock/34. These strains differed in the degree of homology of eight genome fragments, electrophoretic mobility of the majority of proteins, size of plaques and rct42 marker and displayed significant differences in antigenic specificity of haemagglutinin. Strains A/FPV/Weybridge and A/FPV/Dobson proved to be more close in the degree of genome homology but differed in three genes, electrophoretic mobility of some proteins, size of plaques, rct42 marker and antigenic specificity of haemagglutinin.