Clinical Validation of a Targeted RNA-Sequencing Assay for Driver Gene Alteration Detection in Non-Small Cell Lung Cancer.

Background And Objective: With the increasing number of diagnostic biomarkers associated with tumor diagnosis, targeted therapy, and immunotherapy, access to clinical pathological specimens of an appropriate size for analysis is becoming a problem. Conventional high-throughput sequencing assays for non-small cell lung cancer (NSCLC) often necessitate the extraction of separate DNA and RNA samples to achieve precise detection of various mutation types. This study aimed to employ RNA-next-generation sequencing (NGS) technology to simultaneously detect different types of mutations in NSCLC samples, including single nucleotide variations, insertions and deletions, fusions/rearrangements, and exon skipping, thereby addressing the issue of limited sample availability.

PANoptosis-related gene biomarkers in aortic dissection.

Introduction: Programmed cell death of vascular smooth muscle cells (VSMCs) is critical in the pathogenesis of aortic dissection (AD), yet the role of PANoptosis-comprising pyroptosis, apoptosis, and necroptosis-remains unclear.

Methods: We utilized the GSE213740 single-cell sequencing dataset to assess PANoptosis levels in VSMCs. Datasets GSE153434 and GSE147026 were employed to identify differentially expressed genes (DEGs) and perform weighted gene co-expression network analysis.

Phase-transited lysozyme coating on zirconia abutments for enhancing soft tissue seal and antibacterial activity.

Zirconia abutments lacking intrinsic antibacterial properties and exhibiting inadequate sealing at the zirconia abutments-soft tissue interface may cause peri-implantitis, ultimately leading to implant failure. Simultaneously improving the soft tissue seal while integrating the antibacterial properties of zirconia abutments presents significant challenges. A phase-transited lysozyme (PTL) coating was successfully constructed on zirconia abutments to enhance soft tissue attachment and antibacterial properties, aiming to safeguard peri-implant soft tissue from infection.

[Corrigendum] Upregulation of interleukin‑17F in colorectal cancer promotes tumor invasion by inducing epi​thelial‑mesenchymal transition.

Subsequently to the publication of the above paper, an interested reader drew to the authors' and the Editor's attention that the clone image featured in Fig. 4A for the 'NC' (or 'negative control') data panel on p. 1146 had also been featured as the 'NC' experimental data panel in Fig.

MBD2 promotes epithelial-to-mesenchymal transition (EMT) and ARDS-related pulmonary fibrosis by modulating FZD2.

Objective: To investigate the role and underlying mechanism of Methyl-CpG binding domain protein 2 (MBD2) in the pathogenesis of acute respiratory distress syndrome (ARDS)-related pulmonary fibrosis.

Methods: Murine models for ARDS-related pulmonary fibrosis were established in wildtype or MBD2 knockout mice, expressions of MBD2 were determined with immunohistochemistry (IHC), immunofluorescence, and western blot. Epithelial-to-mesenchymal transition (EMT) was detected with determined with decreased expression of E-cadherin and increased expressions of N-cadherin, Vimentin, and α-smooth muscle actin (α-SMA).

Discovery of : A Potent and Highly Selective Irreversible CDK12/13 Inhibitor with Synergistic Effects in Combination with Akt Inhibition.

Cyclin-dependent kinases 12 and 13 (CDK12/13) have emerged as promising therapeutic targets for castration-resistant prostate cancer (CRPC) and other human cancers. Despite the development of several CDK12/13 inhibitors, challenges remain in achieving an optimal balance of potency, selectivity and pharmacokinetic properties. Here, we report the discovery of , a novel, potent and highly selective covalent inhibitor of CDK12/13 with reasonable pharmacokinetic profiles.

Structural Stabilization and Activity Enhancement of Glucoamylase via the Machine-Learning Technique and Immobilization.

Glucoamylases (GLL) hydrolyze starch to glucose syrup without yielding intermediate oligosaccharides, but their lack of stability under industrial conditions poses a major limiting factor. Using consensus- and ancestral-based machine-learning tools, a functional GLL with six mutations (GLL) was constructed that exhibited superior hydrolytic activity relative to the wild-type (WT-GLL). An oxidized multi-walled carbon nanotube (oMW-CNT) was used as a solid support to immobilize the WT-GLL with an immobilization capacity of 211.

-Induced Liver Damage Through Ferroptosis in Rat Model.

(1) Background: (CE) is an -induced worldwide parasitic zoonosis and is a recognized public health and socio-economic concern. The liver is the major target organ for CE's infective form protoscolex (PSCs), which causes serious liver damage and endangers the host's life. Reports show that PSC infection causes liver cell Fe metabolism disorder and abnormal deposition of Fe in liver cells and results in liver cell death.

Distribution and accumulation dynamics of fungicide azoxystrobin in the soil-plant system.

Azoxystrobin is a widely used fungicide belonging to the strobilurin group, primarily employed to control a range of fungal diseases in crops, including pepper (Capsicum annuum L.). As a systemic pesticide, it effectively inhibits mitochondrial respiration in fungi, thereby preventing their growth and spread.

Machine learning-based models for advanced fibrosis in non-alcoholic steatohepatitis patients: A cohort study.

Background: The global prevalence of non-alcoholic steatohepatitis (NASH) and its associated risk of adverse outcomes, particularly in patients with advanced liver fibrosis, underscores the importance of early and accurate diagnosis.

Aim: To develop a machine learning-based diagnostic model for advanced liver fibrosis in NASH patients.

Methods: A total of 749 patients who underwent liver biopsy at Beijing Ditan Hospital, Capital Medical University, between January 2010 and January 2020 were included.

Oxygen/siRNA-carrying fluoro-nanosensitizers for radio-immunotherapy sensitization.

The anti-tumor efficacy of radiotherapy (RT) is limited by the hypoxic and immunosuppressive tumor microenvironment (TME), which leads to RT resistance and failure in eradicating distant metastatic lesions. Herein, we developed a fluorinated nanosensitizer that could deliver both oxygen (O) and ADAR1 siRNA into tumor cells to reinforce RT by alleviating hypoxia and immunosuppression. Fluorinated poly(β-amino ester) (fPBAE) was designed to complex ADAR1 siRNA (siADAR1) via electrostatic attraction and load O due to the O-dissolving capacity of fluoroalkyls.

Maize SERRATE 1B positively regulates seed germinability under low-temperature.

Low temperature poses a significant threat to seedling emergence after maize sowing. While the impact of SERRATE (SE) on plant development via RNA processing has been extensively reported, its involvement in transcriptional regulation or the formation of low-temperature germination ability remains unclear. Our previous research revealed that ZmSE1B is located at the overlapping region of qLTGR4-1 or qLTPRL4-1, which has been associated with low-temperature germination by QTL analysis using IBM Syn4 RIL population.

Time-restricted feeding mitigates HFD-induced sarcopenic obesity in aging mice through improving the sensitivity of FGF21.

Time-restricted feeding (TRF) is a dietary intervention that has been shown to have numerous health benefits. However, it is important to further investigate the potential effectiveness of TRF in addressing sarcopenic obesity (SO), which is characterized by a combination of age-related obesity and sarcopenia. In this study, 14-month-old C57BL/6J male mice were fed either regular chow diet or high-fat diet (HFD), and had either ad libitum or restricted access to food for 8 hours daily (Intervention for 7 months).

Cell-free DNA-scavenging nano/microsystems for immunotherapy.

In the context of inflammation, autoimmune diseases, infections, and cancers, cfDNA plays a pivotal role in disease progression through various mechanisms. Immunotherapies based on cfDNA scavenging has emerged as a promising approach for treating these conditions. This review offers a comprehensive exploration of cfDNA-binding and degradation strategies, providing detailed insights into the corresponding nano/microsystems for each approach.

Hypoxia inducible factor-1α drives cancer resistance to cuproptosis.

Cuproptosis represents a new type of cell death that intricately associated with copper homeostasis and protein lipoylation. The cuproptosis suppression has been characterized in the hypoxic tumor microenvironment (TME). Here we reveal that hypoxia inducible factor-1α (HIF-1α) is a driver of cuproptosis resistance in solid tumor.

Integrated multi-omic characterizations of the synapse reveal RNA processing factors and ubiquitin ligases associated with neurodevelopmental disorders.

The molecular composition of the excitatory synapse is incompletely defined due to its dynamic nature across developmental stages and neuronal populations. To address this gap, we apply proteomic mass spectrometry to characterize the synapse in multiple biological models, including the fetal human brain and human induced pluripotent stem cell (hiPSC)-derived neurons. To prioritize the identified proteins, we develop an orthogonal multi-omic screen of genomic, transcriptomic, interactomic, and structural data.

The amphipod genome reveals population dynamics and adaptations to hadal environment.

The amphipod Hirondellea gigas is a dominant species inhabiting the deepest part of the ocean (∼6,800-11,000 m), but little is known about its genetic adaptation and population dynamics. Here, we present a chromosome-level genome of H. gigas, characterized by a large genome size of 13.

Des-γ-carboxy Prothrombin in hepatocellular carcinoma post-operative recurrence risk evaluation.

Background: While the value of Des-γ-carboxy prothrombin in hepatocellular carcinoma diagnosis has been widely acknowledged, whether or how Des-γ-carboxy prothrombin could be used in recurrence evaluation remains largely unexplored.

Methods: We performed a multicenter retrospective analysis including an Exploration Cohort (1074 patients, 5133 Des-γ-carboxy prothrombin measurements) and a Validation Cohort (263 patients, 612 Des-γ-carboxy prothrombin measurements) to investigate whether Des-γ-carboxy prothrombin could evaluate patients' prognosis. We introduced the Des-γ-carboxy prothrombin dynamic rate as a normalized quantitative measurement of Des-γ-carboxy prothrombin dynamic change.

SLC31A1 loss depletes mitochondrial copper and promotes cardiac fibrosis.

Background And Aims: Metals serve as co-factors for a host of metalloenzymes involved in mitochondrial metabolic reprogramming. Modifications in metal homeostasis are linked to epigenetic mechanisms. However, the epigenetic mechanisms through which metal affects cardiac fibrosis (CF) remain poorly understood.

Vibration Analysis of Al-AlO Micro-Cantilever Sandwich Beams with Porosity in Fluids.

The vibration of porous Al-AlO micro-cantilever sandwich beams in fluids was studied utilizing the modified couple stress theory and the scale distribution theory (MCST and SDT). Four types of porosity distributions were defined; the uniform distribution of pores was defined as U-type, while O-type, V-type and X-type represented non-uniform distributions of pores. The material properties of different porous sandwich beams were calculated.

Practice of core competence training for orthopedic nurses in Hunan Province of China.

Objectives: This study aims to present a preliminary exploration of an orthopedic nurse specialist training program in Hunan Province, China, and to explore the effectiveness of training.

Methods: The training programme consisted of one month of theoretical training and two months of clinical practice training. The training content was set around four modules: theoretical knowledge, professional skills, comprehensive practice, and professional development.

DSLE2 random-effects meta-analysis model for high-throughput methylation data.

Background: With the rapid development of high-throughput sequencing technology, high-throughput sequencing data has grown on a massive scale, leading to the emergence of multiple public databases, such as EBI and GEO. Conducting secondary mining of high-throughput sequencing data in these databases can yield more valuable insights. Meta-analysis can quantitatively combine high-throughput sequencing data from the the same topic.