Comparison of the Distribution of Lenticule Decentration Following SMILE by Pupil Center or Tear Film Mark Centration.

Purpose: To investigate lenticule decentration following small incision lenticule extraction (SMILE) via the pupil center or tear film mark centration method and compare induction of corneal higher order aberrations (HOAs) between the two methods.

Methods: This study analyzed decentration values obtained from tangential topography difference maps of 100 eyes (100 patients) undergoing SMILE with the pupil center (n = 50) or tear film mark (n = 50) centration method. Total HOAs and component aberrations were measured preoperatively and 6 months postoperatively.

The therapeutic potential of bilobalide on experimental autoimmune encephalomyelitis (EAE) mice.

Inflammatory demyelination in the central nervous system (CNS) is a hallmark of multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE). Besides MS disease-modifying therapy, targeting myelin sheath protection/regeneration is currently a hot spot in the treatment of MS. Here, we attempt to explore the therapeutic potential of Bilobalide (BB) for the myelin protection/regeneration in EAE model.

Molecular Characterization and Expression Profiles of the Ovine Gene and Its Association with Litter Size in Chinese Indigenous Small-Tailed Han Sheep.

The luteinizing hormone beta polypeptide (LHβ) is a glycoprotein hormone secreted by basophilic granular cells of the adenohypophysis, and plays an important role in mammalian follicular development. In this study, we cloned and analyzed the cDNA sequence of the ovine gene. RT-qPCR analysis showed that ovine was widely expressed in tissues, with significantly higher expression in the hypophysis than that in other tissues (heart, liver, spleen, lung, kidney, rumen, duodenum, muscle, fat, hypothalamus, and sex glands) ( < 0.

Hierarchical CuO@ZnCo LDH heterostructured nanowire arrays toward enhanced water oxidation electrocatalysis.

It is of great importance to design and develop complex heterostructured nanocatalysts with superior electrochemical performance to that of single structured ones. Here, we report the hydrothermal fabrication of a hierarchical heterostructured CuO@ZnCo layered double hydroxide nanowire array on a copper foil (CuO@ZnCo LDH/CF). As a self-supported electrocatalyst for water oxidation, CuO@ZnCo LDH/CF has superior catalytic activity with the requirement of a low overpotential of 270 mV to attain 10 mA cm-2 in 1.

Proteomic Analysis of Beef Tenderloin and Flank Assessed Using an Isobaric Tag for Relative and Absolute Quantitation (iTRAQ).

Herein, we performed a proteomic analysis of tenderloin and flank steaks from Simmental cattle using the isobaric tags for a relative and absolute quantification (iTRAQ) approach. We identified 17 amino acids in both steaks, and Gly, Cys, Ile, Lys, and Pro differed most in abundance between the steak types ( < 0.05).

Oroxindin inhibits macrophage NLRP3 inflammasome activation in DSS-induced ulcerative colitis in mice via suppressing TXNIP-dependent NF-κB pathway.

Oroxindin is a flavonoid isolated from the traditional Chinese medicine Huang-Qin, which has shown various pharmacological activities including anti-inflammatory, antitumor, antioxidant, etc. Thus far, the effect of oroxindin on colonic inflammation and the underlying mechanism remain unknown. In this study, we investigated the tissue distribution of oroxindin and its therapeutic effects on ulcerative colitis (UC) as well as the underlying mechanisms.

Stable reduction of STARD4 alters cholesterol regulation and lipid homeostasis.

STARD4, a member of the evolutionarily conserved START gene family, is a soluble sterol transport protein implicated in cholesterol sensing and maintenance of cellular homeostasis. STARD4 is widely expressed and has been shown to transfer sterol between liposomes as well as organelles in cells. However, STARD4 knockout mice lack an obvious phenotype, so the overall role of STARD4 is unclear.

Resveratrol loaded glycyrrhizic acid-conjugated human serum albumin nanoparticles for tail vein injection II: pharmacokinetics, tissue distribution and bioavailability.

There are many kinds of biological activities of resveratrol itself, but its clinical application is limited by its poor solubility in water and low bioavailability. Therefore, we have prepared glycyrrhizic acid-conjugated human serum albumin nanoparticles wrapping resveratrol nanoparticles (GL-HSA-RESNPs). The purpose of this study was to investigate the bioavailability, pharmacokinetics and tissue distribution of resveratrol in rats after single-dose tail vein injection administration of GL-HSA-RESNPs.

Primary sclerosing cholangitis with increased immunoglobulin G4 levels: A case report.

Rationale: Primary sclerosing cholangitis (PSC) is recognized as an autoimmune-mediated liver disease characterized by progressive biliary inflammation and fibrosis. Some PSC cases with elevated immunoglobulin G4 (IgG4) levels are likely to be misdiagnosed with immunoglobulin G4-related sclerosing cholangitis (IgG4-SC). Thus, distinguishing these 2 diseases is particularly important.

Regulation of Recurrent Inhibition by Asynchronous Glutamate Release in Neocortex.

The timing and size of inhibition are crucial for dynamic excitation-inhibition balance and information processing in the neocortex. The underlying mechanism for temporal control of inhibition remains unclear. We performed dual whole-cell recordings from pyramidal cells (PCs) and nearby inhibitory interneurons in layer 5 of rodent neocortical slices.

Derivation and external validation of a risk prediction algorithm to estimate future risk of cardiovascular death among patients with type 2 diabetes and incident diabetic nephropathy: prospective cohort study.

Objective: To derive, and externally validate, a risk score for cardiovascular death among patients with type 2 diabetes and newly diagnosed diabetic nephropathy (DN).

Research Design And Methods: Two independent prospective cohorts with type 2 diabetes were used to develop and externally validate the risk score. The derivation cohort comprised 2282 patients with an incident, clinical diagnosis of DN.

Transcriptome Analysis Identifies Candidate Genes and Pathways Associated With Feed Efficiency in Hu Sheep.

In the genetic improvement of livestock and poultry, residual feed intake (RFI) is an important economic trait. However, in sheep, the genetic regulatory mechanisms of RFI are unclear. In the present study, we measured the feed efficiency (FE)-related phenotypes of 137 male Hu lambs, and selected six lambs with very high (n = 3) and very low (n = 3) RFI values and analyzed their liver transcriptomes.

Silver nanoparticles inhibit denitrification by altering the viability and metabolic activity of Pseudomonas stutzeri.

The environmental toxicity of silver nanoparticles (AgNPs) is currently the focus of intensive research. However, the mechanisms underlying the cytotoxic effects of AgNPs on denitrifying microbes have yet to be explicitly demonstrated. Herein, Pseudomonas stutzeri was used to explore the effects of AgNPs on denitrification and cytotoxicity.

LncRNA SPRY4-IT1 regulates breast cancer cell stemness through competitively binding miR-6882-3p with TCF7L2.

SPRY4-intronic transcript 1 has been found in several kinds of cancers, but the role of SPRY4-IT1 in breast cancer stem cells has not been studied. We investigated whether SPRY4-IT1 is involved in the promotion of breast cancer stem cells (BCSCs). We used qRT-PCR to detect the expression of SPRY4-IT1 in MCF-7 cells and MCF-7 cancer stem cells (MCF-7 CSCs).

Characteristics of a Colistin-Resistant ST695 Harboring the Chromosomally-Encoded m Gene.

having chromosomally-encoded is rarely reported. In this study, we recovered a chromosomal carrying , designated HeN100, from the feces of a diarrheal pig in China. Antimicrobial susceptibility testing showed that HeN100 was resistant to three aminoglycosides, twelve β-lactams including three carbapenems, one phenicol, two tetracyclines, two fluoroquinolones, nitrofurantoin, and colistin tested.

Involvement of P2X7 receptors in satellite glial cells of dorsal root ganglia in the BmK I -induced pain model of rats.

The P2X7 receptor (P2X7R) plays an important role in inflammatory and neuropathic pain. Our recent study indicated that activation of P2X7R in microglial cells of spinal cord contributes to the inflammatory pain induced by BmK I, the major active compound from Buthus martensi Karsch (BmK). In the present study, we further investigated whether P2X7R in satellite glial cells (SGCs) of dorsal root ganglion (DRG) is involved in the BmK I-induced pain in rats.

Molecular Characterization and Expression of , and and Their Association with Growth Traits in Sheep.

The , , and are located on chromosome 6 of sheep and a domain of 36.15-38.56 Mb, which plays an essential role in tissue and embryonic growth.

Loading paclitaxel into porous starch in the form of nanoparticles to improve its dissolution and bioavailability.

In this work, paclitaxel was loaded into porous starch in the form of nanoparticles (PNPS), and the properties of PNPS were investigated by using raw paclitaxel and the system of paclitaxel directly loaded into porous starch (PPS) as control groups. According to the tested results, the drug loading (DL) and encapsulation efficiency (EE) of PNPS were 14.13%±0.

Optimal cut-off threshold in pulse pressure predicting cardiovascular death among newly diagnosed end-stage renal disease patients: A prospective cohort study.

Cardiovascular disease (CVD) is the leading cause of death in patients with end-stage renal disease (ESRD) treated by dialysis. Pulse pressure (PP) as an independent prognostic factor of cardiovascular risk might be clinically implicated in predicting the short-term deaths due to cardiovascular diseases in ESRD patients. This study aimed to investigate the dose-response association between PP and risk of cardiovascular mortality in patients initializing peritoneal dialysis (PD).

[The risk factors and preventive measures of hospital infections on preoperative children with cleft lips and palates].

Objective: To analyze the risk factors of preoperative upper respiratory infections in children with cleft lips and palate (CLP) and investigate preventive measures to reduce infections and improve the quality of treatments.

Methods: A total of 510 children with CLP of ages 3 years old or younger were selected from hospital cases from June to December 2017. The test group comprised 50 children with upper respiratory infections, whereas the control group comprised 460 children without upper respiratory infections.

WO nanosheets rich in oxygen vacancies for enhanced electrocatalytic N reduction to NH.

The Haber-Bosch process for industrial-scale NH3 production suffers from harsh conditions and serious CO2 release. Electrochemical N2 reduction is an alternative approach to synthesize NH3 under ambient conditions, but it requires highly-efficient electrocatalysts for the N2 reduction reaction (NRR). In this Communication, we demonstrate that WO3 nanosheets rich in oxygen vacancies (R-WO3 NSs) exhibit greatly enhanced NRR performances.

[Investigation on knowledge, attitude and practice (KAP) of foodborne parasitic diseases among medical students].

Objective: To understand the knowledge, attitude and practice (KAP) of foodborne parasitic diseases among medical college students, so as to provide the evidence for the development of strategies on health education.

Methods: A total of 929 premed students from a medical college were selected in Hebei Province using the stratified sampling method from October to December, 2017, and investigated by questionnaires to understand their KAP on foodborne parasitic diseases.

Results: The understanding and concern degree on foodborne parasitic diseases among the college students from different majors were statistically different (all < 0.

A fluorescein-based AND-logic FPSi probe for the simultaneous detection of Hg and F.

In this study, we have designed and synthesized a novel 'AND' logic based fluorescence probe,1-(3',6'-bis((tert-butyldiphenylsilyl)oxy)-3-oxospiro (isoindoline-1,9'-xanthen)-2-yl)-3-phenylthiourea (FPSi), for the rapid (3 min) simultaneous detection of F and Hg in DMSO/HO solution (7:3, v/v). The FPSi probe, synthesized over three steps starting from commercially available fluorescein, was constructed by attaching tert-butyldiphenylsilyl and thiosemicarbazide (as the specific identification groups for F and Hg) to the skeleton of fluorescein, respectively. FPSi produced no fluorescence response towards the addition of F or Hg separately.

Deep Genome Resequencing Reveals Artificial and Natural Selection for Visual Deterioration, Plateau Adaptability and High Prolificacy in Chinese Domestic Sheep.

Sheep were one of the earliest domesticated animals. Both artificial and natural selection during domestication has resulted in remarkable changes in behavioral, physiological, and morphological phenotypes; however, the genetic mechanisms underpinning these changes remain unclear, particularly for indigenous Chinese sheep. In the present study, we performed pooled whole-genome resequencing of 338 sheep from five breeds representative of indigenous Chinese breeds and compared them to the wild ancestors of domestic sheep (Asian mouflon, ) for detection of genome-wide selective sweeps.

Relationship between psychosocial adaptation and health-related quality of life of patients with stoma: A descriptive, cross-sectional study.

Objective: To investigate the relationship between psychosocial adaptation and quality of life of patients with ostomies and to provide reference data for clinical care practice.

Background: Surgical enterostomy impacts the life of patient. Most patients are transferred to home or community hospital after discharge and require long-term nursing care.