Abamectin Causes Neurotoxicity in Zebrafish Embryos.

Abamectin is an insecticide, miticide and nematicide that has been extensively used in agriculture for many years. The excessive use of abamectin inevitably pollutes water and soil and might even cause adverse effects on aquatic biota. However, it is currently unclear how abamectin exposure causes neurotoxicity in aquatic organisms.

DNA Tetrahedron Reformative Lateral Flow Assay for Improved Detection Sensitivity and Anti-Interference.

The lateral flow assay is a strip-based analytical method for the portable and convenient detection of analytes of interest. It has the advantages of visual observation, autonomous sample flow, fast coloration time, minimal tedious operation procedures, and reliance on specialized instruments. However, the rough surface of the nitrocellulose membrane renders it difficult for the immobilized nucleic acids to remain in an ordered arrangement, and the immobilized nucleic acids are also liable to be digested in a complex matrix, inducing limited sensitivity and anti-interference.

Calcium alginate reinforced zwitterionic double network hydrogel with mechanical robustness and antimicrobial activity for freshwater shrimp spoilage detection.

Hydrogel indicators promise to monitor food spoilage, but their poor mechanics can cause defects in transport. Herein, a novel zwitterionic double network (DN) hydrogel was developed by polymerizing arylamide and sulfobetaine methacrylate in an alginate-Ca system. This hydrogel exhibited enhanced mechanical properties, including a maximum 2087 % breaking elongation and 135 ± 12 kJ/m toughness, significantly outperforming the current zwitterionic DN hydrogels, which typically exhibit less than 1800 % breaking elongation, capable of supporting 150 g-136 times its own weight.

Insights into the Genomic Background of Nine Common Chinese Medicinal Plants by Flow Cytometry and Genome Survey.

Medicinal plants have long played a crucial role in healthcare systems, but limited genomic information on these species has impeded the integration of modern biological technologies into medicinal plant research. In this study, we selected nine common medicinal plants, each belonging to a different plant family, including (Chloranthaceae), (Vitaceae), (Fabaceae), (Cucurbitaceae), (Polygonaceae), (Caryophyllaceae), (Rubiaceae), (Lamiaceae), and (Asteraceae), to estimate their genome sizes and conduct preliminary genomic surveys. The estimated genome sizes by flow cytometry were 3.

Tetrachlorantraniliprole induces neurodevelopmental toxicity through oxidative stress-mediated apoptosis and dysregulation of Wnt signaling pathway.

Tetrachlorantraniliprole (TCTP) is a novel bisamide insecticide and widely used to protect against lepidopteran insect species. However, the application of TCTP in rice fields often leads to water pollution, posing threats to aquatic organisms and potentially to human health. Few studies have assessed the toxic effects of TCTP on aquatic animals.

An oral probiotic vaccine loaded by Lactobacillus casei effectively increases defense against GCRV infection in grass carp.

For a long time, grass carp culture in China has been severely affected by Grass Carp hemorrhagic disease caused by Grass Carp Reovirus (GCRV). At present, vaccines have been widely used for protecting aquatic organisms against infectious diseases, among which oral immunization with Lactobacillus casei is safe and highly effective. This vaccination route has the advantages of easy administration and noninvasive delivery.

The GRAS gene family and its roles in pineapple (Ananas comosus L.) developmental regulation and cold tolerance.

Background: Pineapple (Ananas comosus L.) is a major tropical fruit crop with considerable economic importance, and its growth and development are significantly impacted by low temperatures. The plant-specific GRAS gene family plays crucial roles in diverse processes, including flower and fruit development, as well as in stress responses.

Microbial diversity and environmental determinants at Shanghai Hongqiao railway station: A comprehensive microbial assessment.

The COVID-19 pandemic has underscored the importance of indoor environmental management in transportation hubs, which are critical for pathogen transmission due to high foot traffic. However, research has primarily focused on subways, with limited studies on train stations. In this study, samples were collected at the Shanghai Hongqiao Railway Station in winter, spring, and summer.

[Effect of electroacupuncture on neurological function in rats with cerebral ischemia-reperfusion injury based on AMPK/mTOR/ULK1 signaling pathway].

Objective: To investigate the effect of electroacupuncture (EA) on neurological function in rats with cerebral ischemia-reperfusion injury (CIRI) by regulating adenosine monophosphate-activated protein kinase (AMPK)/mammalian target of rapamycin (mTOR)/Unc-51-like kinase 1 (ULK1) signaling pathway.

Methods: Thirty-three male SD rats were randomly divided into a sham-operation group, a model group and an EA group, with 11 rats in each group. The right middle cerebral artery occlusion/reperfusion (MCAO/R) model was prepared by thread occlusion method in the model group and the EA group.

GCRV-II major outer capsid protein VP4 promotes cell apoptosis by VDAC2-mediated calcium pathway facilitation.

Grass Carp Reovirus (GCRV) is widely concerned because of its widespread prevalence and high mortality to grass carp (Ctenopharyngodon idellus). Viral protein 4 (VP4) is an important major outer capsid protein of GCRV and is involved in the regulation of cell cycle and cycle of GCRV replication. However, the elaborate function of VP4 remains to be explicated.

Outcome prediction comparison of ischaemic areas' radiomics in acute anterior circulation non-lacunar infarction.

The outcome prediction of acute anterior circulation non-lacunar infarction (AACNLI) is important for the precise clinical treatment of this disease. However, the accuracy of prognosis prediction is still limited. This study aims to develop and compare machine learning models based on MRI radiomics of multiple ischaemic-related areas for prognostic prediction in AACNLI.

Zebrafish FKBP5 facilitates apoptosis and SVCV propagation by suppressing NF-κB signaling pathway.

FK506-binding protein 5 (FKBP5), encoded by FKBP5 gene, has been reported as a scaffolding protein in various mammalian pathways related to immunity, inflammation, apoptosis and autophagy. However, the role of FKBP5 in lower vertebrates remains unknown. In this study, we identified zebrafish FKBP5 (DrFKBP5), an ortholog of mammalian FKBP5, which shows high homology with its counterpart in Anabarilius grahami based on amino acid alignment and phylogenetic analysis.

Polyacrylic Acid-Reinforced gelatin hydrogels with enhanced mechanical properties, temperature-responsiveness and antimicrobial activity for smart encryption and salmon freshness monitoring.

Hydrogels hold great potential for use in intelligent packaging, yet they often suffer from limited functionality and inadequate mechanical strength when applied to anticounterfeiting and freshness monitoring. In this study, we present a straightforward method to create a multifunctional hydrogel by in-situ polymerizing acrylic acid (PAA) within a gelatin-Al system. The resulting hydrogels exhibited an elongation at break of over 1200 %, a tensile stress of 1.

COVID-19 Vaccine Information Exposure: The Effect of Online Authority vs. Non-Authority Sources on Beliefs, Emotions and Information Engagement Behaviors.

Limited research has examined the theoretical linkages between exposure to COVID-19 vaccine information sources, vaccination-related beliefs, vaccination-induced emotions, and vaccine information engagement. An online survey was conducted with a national sample of adults (N = 630) residing in the U.S.

Use of a Space Graft in Rhinoplasty.

Background: Construction of the nasal tip strut is an important part of rhinoplasty; the incidence of postoperative complications is closely related to the type of graft and the approach to cartilaginous framework construction.

Objectives: To introduce a supplementary graft to support the cartilaginous framework.

Methods: Forty patients (37 female and 3 male), aged from 18 to 40 years (average 29), received a rhinoplasty using a spacer graft.

Cortical Thickness and Complexity in aMCI Patients: Altered Pattern Analysis and Early Diagnosis.

Background: Amnestic Mild Cognitive Impairment (aMCI) is a prodromal phase of Alzheimer's disease. Although recent studies have focused on cortical thickness as a key indicator, cortical complexity has not been exhaustively investigated.

Objectives: To investigate the altered patterns of cortical features in aMCI patients and their correlation with memory function for early identification.