Doublecortin regulates the mitochondrial-dependent apoptosis in glioma via Rho-A/Net-1/p38-MAPK signaling.

Doublecortin (DCX) is a microtubule-associated protein known to be a key regulator of neuronal migration and differentiation during brain development. However, the role of DCX, particularly in regulating the survival and growth of glioma cells, remains unclear. In this study, we utilized CRISPR/Cas9 technology to knock down DCX in the human glioma cell line (U251).

Research on sleep disorders and related risk factors among healthcare workers from Fujian province supporting Hubei province during the COVID-19 pandemic.

Objective: To explore the impact of COVID-19 on the sleep of healthcare workers from Fujian Province supporting Hubei Province and its related risk factors.

Methods: A cross-sectional, anonymous, self-reported online questionnaire survey was conducted among all participants. The questionnaire consisted of five parts: sociodemographic characteristics and COVID-19 epidemic-related factors, Pittsburgh Sleep Quality Index (PSQI), Epworth Sleepiness Scale (ESS), Morningness-Eveningness Questionnaire-5 (MEQ-5), and 12-item General Health Questionnaire (GHQ-12).

Anti-inflammatory mechanism of the non-volatile ingredients originated from Guanghuoxiang () based on high performance liquid chromatography-heated electron spray ionization-high resolution mass spectroscope and cell metabolomics.

Objective: To explore the anti-inflammatory components and mechanism of the non-volatile ingredients of patchouli.

Methods: High performance liquid chromatography-heated electron spray ionization-high resolution mass spectroscope (HPLC-HESI-HRMS) was used to analyze the chemical constituents of the non-volatile ingredients of patchouli. The anti-inflammatory activity of ingredients was evaluated using lipopolysaccharide (LPS) induced RAW264.

Combination of allopurinol with Dahuang Mudan Tang significantly improve kidney function and alleviate oxidative stress and inflammation of chronic kidney disease stage Ⅰ-Ⅲ patients with hyperuricemia.

Objective: To evaluate the effect of Dahuang Mudan Tang (, DHMD) and allopurinol on the treatment of chronic kidney disease staged G1-G3b patients with hyperuricemia and to provide novel insights into the clinical management of chronic kidney disease complications.

Methods: A total of 80 chronic kidney patients staged G1-G3b with hyperuricemia were randomly grouped to receive single allopurinol treatment (control) and combined treatment with DHMD (treated) for 8 weeks. The kidney function and proteinuria indicators of patients were compared between pre-and post-treatment.

Evaluation of theTherapeutic Effect of the Hybrid Operation on Patients with Aneurysms Associated with Cerebral Arteriovenous Malformations with different Redekop Classifications.

Background: Intracranial aneurysms associated with cerebral arteriovenous malformations (AVMs) are a rare condition in the clinic, and treatment is very difficult due to their particular anatomical features. We present our experience in the treatment of intracranial aneurysms with AVMs and evaluate the effectiveness and safety of endovascular treatment combined with microsurgical resection (the hybrid operation).

Methods: This was a single-center retrospective study in our neurosurgical department from January 2015 to January 2021.

Fabrication and characterization of porous titanium-based PbO electrode through the pulse electrodeposition method: Deposition condition optimization by orthogonal experiment.

Porous titanium-based PbO electrodes were successfully fabricated by pulse electrodeposition method. The primary pulse electrodeposition parameters, including pulse frequency (f), duty ratio (γ), average current density (J) and electrodeposition time (t) were considered in this study. An orthogonal experiment was designed based on those four factors and in three levels.

CT-guided microcoil implantation for localizing pulmonary ground-glass nodules: feasibility and accuracy of oblique approach for lesions difficult to access on axial images.

Objective: (1) To evaluate the value of CT-guided microcoil implantation for localizing pulmonary ground-glass nodules (GGNs) before video-assisted thoracoscopic surgery (VATS). (2) To evaluate the feasibility, safety and accuracy of cephalic-caudal oblique approach for lesions difficult to access on axial images owing to overlying bony structures, large vessels or interlober fissures.

Methods: From June 2016 to March 2019, all patients with GGNs resected by VATS after marking using CT-guided microcoil implantation in China-Japan friendship hospital were enrolled and clinical and imaging data were retrospectively analyzed.

Genomic characterisation of a colistin-resistant Klebsiella pneumoniae ST11 strain co-producing KPC-2, FloR, CTX-M-55, SHV-12, FosA and RmtB causing a lethal infection.

Objectives: The aim of this study was to characterise the genomic and phenotypic characteristics of a colistin-resistant Klebsiella pneumoniae isolate causing a lethal infection that was phenotypically resistant to carbapenems and colistin.

Methods: Whole-genome sequencing was performed on an Illumina HiSeq 2500 platform. Genome annotation was performed by the Rapid Annotation using Subsystem Technology (RAST) server.

Liraglutide inhibits the apoptosis of human nucleus pulposus cells induced by high glucose through PI3K/Akt/caspase-3 signaling pathway.

Diabetes mellitus (DM) is a potential etiology of disc degeneration. Glucagon-like peptide-1 (GLP-1) is currently regarded as a powerful treatment option for type 2 diabetes. Apart from the beneficial effects on glycaemic control, GLP-1 has been reported to exert functions in a variety of tissues on modulation of cell proliferation, differentiation, and apoptosis.

FBX8 degrades GSTP1 through ubiquitination to suppress colorectal cancer progression.

F-box only protein 8 (FBX8), as a critical component of the SKP1-CUL1-F-box (SCF) E3 ubiquitin ligases, has been associated with several malignancies through interacting with a member of proteins. However, the substrates of FBX8 for destruction in the progression of colorectal carcinoma (CRC) need to be explored. Here, we show that loss of FBX8 accelerates chemical-induced colon tumorigenesis.

Self-standing LiMnNiO/graphene membrane as a binder-free cathode for Li-ion batteries.

Lithium-rich transition-metal layered oxides (LROs), such as LiMnNiO, are promising cathode materials for application in Li-ion batteries, but the low initial coulombic efficiency, severe voltage fade and poor rate performance of the LROs restrict their commercial application. Herein, a self-standing LiMnNiO/graphene membrane was synthesized as a binder-free cathode for Li-ion batteries. Integrating the graphene membrane with LiMnNiO forming a LiMnNiO/graphene structure significantly increases the surface areas and pore volumes of the cathode, as well as the reversibility of oxygen redox during the charge-discharge process.

Elevated production of IL-36α in chronic hepatitis B virus-infected patients correlates with viral load.

Chronic hepatitis B (CHB) infection is a typical inflammatory disease characterized by a dysregulated expression of cytokines, which contributes to the pathogenesis of chronic Hepatitis B virus (HBV) infection. IL-36 cytokines (IL-36α, IL-36β, IL-36γ and IL-36Ra) are important players in infection and immunity. However, their roles in the pathogenesis of chronic HBV infection remain unknown.

Value of whole-body magnetic resonance imaging for screening multifocal osteonecrosis in patients with polymyositis/dermatomyositis.

Objective: To assess the value of coronal short-tau inversion recovery whole-body MRI (STIR-WBMRI) for screening osteonecrosis in patients with polymyositis (PM)/dermatomyositis (DM).

Methods: The imaging and medical records of 129 patients with PM/DM who met the Bohan and Peter diagnostic criteria were retrospectively analyzed. STIR-WBMRI was performed in all patients.

DUSP1 inhibits cell proliferation, metastasis and invasion and angiogenesis in gallbladder cancer.

DUSP1/MKP1 is a dual-specific phosphatase that regulates MAPK activity and is known to play a key role in tumor biology. Its function in gallbladder cancer (GBC) remains largely unknown, however. By exploring its activities in two GBC cell lines (SGC996 and GBC-SD), DUSP1 was found to inhibit GBC cell proliferation, migration and invasion.

[Repair of lower extremity soft tissue defect with free musculo-cutaneous flaps bridging with healthy contralateral posterior tibial vessel].

Objective: To observe the clinical effects of free musculo-cutaneous flap bridging with contralateral posterior tibial vessel on repair of lower extremity soft tissue defect.

Methods: From February 2006 to June 2013, 10 patients with soft tissue defect on lower shank and foot were included. The posterior tibial vessel on healthy lower extremity was chosen as recipient vessel and anastomosed with free latissimus dorsi musculo-cutaneous flap, or free latissimus dorsi musculo-cutaneous flap combined with thoracic-umbilical skin flap or anterolateral femoral musculo-cutaneous flap.

Identification of two novel rabbit hemorrhagic disease virus (RHDV) B cell epitopes and evaluation of its immunoprotection against RHDV.

The VP60 protein of rabbit hemorrhagic disease virus (RHDV) is a structural protein with important roles in viral replication and assembly. In this study, we immunized BALB/c mice with the RHDV-TP strain. Six monoclonal antibodies (mAbs) were selected and characterized by enzyme-linked immunosorbent assay, Western blotting, and indirectly immunofluorescence analysis (IFA).

Genetic polymorphism of matrix metalloproteinase-1 and coronary artery disease susceptibility: a case-control study in a Han Chinese population.

Coronary artery disease (CAD) receives intensive research due to its high incidence and severe impact on the quality of life. One member of the matrix metalloproteinases (MMPs), MMP-1, has been reported to be associated with CAD. To identify the markers contributing to the genetic susceptibility to CAD, nine single-nucleotide polymorphisms (rs1799750, rs498186, rs475007, rs514921, rs494379, rs996999, rs2071232, rs1938901, and rs2239008) throughout the MMP-1 gene were genotyped using MALDI-TOF within the MassARRAY system, and the allele and genotype distributions were compared between 438 healthy controls and 411 patients with CAD from a Chinese Han population.

[Screening of azoospermia factor microdeletions on Y chromosome in infertile men by QF-PCR].

To assess the application of quantitative fluorescent polymerase chain reaction (QF-PCR) on rapid screening of azoospermia factor (AZF) microdeletions, 1218 infertile men with non-obstructive azoospermia or oligospermia were detected for 9 sequence tagged sites (STSs) in AZF region by multiplex QF-PCR combined with capillary electrophoresis. AMEL (amelogenin) as well as SRY (sex-determining region of Y chromosome) located on short arm of sex chromosome was selected as internal control. Karyotyping was performed on Giemsa-banded metaphase chromosomes of peripheral blood lymphocytes.

Application of hydration reaction on the removal of recalcitrant contaminants in leachate after biological treatment.

Leachate contains amounts of non-biodegradable matters with COD range of 400-1500mg/L after the biological treatment, and should be removed further to attain the Chinese discharge standards. Hydration reaction has the potential to combine and solidify some recalcitrant substances, and thus could be applied as the advanced leachate treatment process. It was found that COD and NH3N decreased from 485 to<250mg/L and 91 to 10mg/L, with the removal rate over 50% and 90% in the first 6d, respectively, and COD and NH3N removal capacity were around 23.

Relationship between high density of peritumoral lymphatic vessels and biological behavior of cervical cancer.

Objective: To investigate the relationship between lymphangiogenesis and lymphatic metastasis in cervical squamous carcinoma.

Methods: Eighty cases of invasive cervical squamous cancer were selected as objects of our study. Double immunohistochemical staining with antibodies against lymphatic vessel endothelial hyaluronan receptor 1 and Ki-67 was used to label the lymphatic vessels and mark the proliferative lymphatic vessels in cervical squamous cancer.

Nanoscaled ZnO films used as enhanced substrates for fluorescence detection of dyes.

The ability of nanoscaled ZnO films to enhance fluorescence was studied. We found that the fluorescence intensities of Cy5, rhodamine 6G, and fluorescein can be enhanced about 10-fold on nanoscaled ZnO films as compared to that on glass substrates. The lifetimes of all samples were measured, and no obvious change in lifetime was observed for dyes on different substrates.

siRNA-mediated knockdown of FoxP3 promotes the ratio of T-helper 1 (Th1) to Th2 in chronic hepatitis B patients.

To determine the effect of regulatory T cells on the ratio of Th1/Th2 differentiation in peripheral blood mononuclear cells of chronic hepatitis B patients, FoxP3, the essential transcription factor for Tcells differentiation and function, was knocked down by FoxP3 siRNA, which was affirmed by real-time polymerase chain reaction for decreased expression of FoxP3. Then, at different time points, comparing with control groups, we detected enhanced lymphocyte proliferation and up-regulated Th1-type cytokines, but down-regulated Th2-type cytokines by ELISA. Finally, to research the involved mechanisms, an increased ratio of T-bet/GATA-3 mRNA expression was found by real-time polymerase chain reaction.

Right ventricular and left atrial and left ventricular myxomas: report of a case.

Myxomas are common cardiac tumors, which are usually managed by complete excision. We report a case of myxomas at different stages of development found simultaneously in the left atrium and both ventricles. The patient underwent successful surgical excision of all myxomas and there has been no clinical or echocardiographic evidence of recurrence or valvular insufficiency in 6 months of follow-up.

Stability of active ingredients of traditional Chinese medicine (TCM).

Studies on stability of active ingredients are fundamental and critical for the rational development of Traditional Chinese Medicine (TCM) in view of its modernization and worldwide use. The stability of both active and marker constituents of plants used in TCM is reviewed for the first time. More than 100 papers, mostly written in Chinese, have been reviewed.