The Role of Mitochondrial DNA Copy Number in Venous Thromboembolism: Insights from a 2-Sample Mendelian Randomization Study.

Background: Venous thromboembolism (VTE), including pulmonary embolism (PE) and deep vein thrombosis (DVT), is the third most common cardiovascular disease. A low amount of mitochondrial DNA copy number (mtDNA-CN) reflects mitochondrial dysfunctions and has been associations with arterial cardiovascular diseases. However, the role of mtDNA-CN in venous cardiovascular disease was unclear.

Research on the unsteady flow characteristics of high specific speed axial flow impellers with small aspect ratio and double blades.

High specific speed impeller with low aspect ratio is ideal for high-speed pump-jet propulsion. This study investigates its unsteady hydrodynamic characteristics using experimental and numerical methods. At the design flow rate (Q), the pressure amplitude at the blade's leading and trailing edges varies significantly along the streamline.

Preparation, characterization and application of chitosan/thyme essential oil composite film.

In order to prepare a chitosan-based antibacterial film with excellent mechanical properties and study its properties, the tape casting method was used to prepare the composite film from chitosan, glycerin and thyme essential oil. Through single factor test and response surface optimization test, the tensile strength and elongation at break were used as response indicators to prepare the composite film with better mechanical properties, and the physical properties, water vapor permeability, solubility and bacteriostasis of the composite film were measured, and the characterization of the microstructure of the composite films. The results showed that the optimized composite film ratio was 1.

Overexpression of ornithine decarboxylase 1 mediates the immune-deserted microenvironment and poor prognosis in diffuse large B-cell lymphoma.

Background: Previous researches mainly focused on whether cancer stem cells exist in diffuse large B-cell lymphoma (DLBCL). However, subgroups with dismal prognosis and stem cell-like characteristics have been overlooked.

Methods: Using large scale data ( = 2133), we conducted machine learning algorithms to identify a high risk DLBCL subgroup with stem cell-like features, and then investigated the potential mechanisms in shaping this subgroup using transcriptome, genome and single-cell RNA-seq data, and experiments.

Doxorubicin-Induced Cardiac Remodeling: Mechanisms and Mitigation Strategies.

Background: The therapeutic prowess of doxorubicin in oncology is marred by its cardiotoxic consequences, manifesting as cardiac remodeling. Pathophysiological alterations triggered by doxorubicin include inflammatory cascades, fibrotic tissue deposition, vascular and valvular changes, and finally cardiomyopathy. These multifarious consequences collectively orchestrate the deterioration of cardiac architecture and function.

Association of social isolation and loneliness with all-cause and cause-specific mortality among individuals with type 2 diabetes: a prospective study in UK Biobank.

Background: Previous study revealed that social isolation and loneliness are associated with incident type 2 diabetes mellitus (T2DM). However, the long-term effects of social isolation and loneliness on the population with T2DM have not been known. This study aims to evaluate the potential associations of social isolation and loneliness with all-cause and cause-specific mortality in individuals with T2DM.

Effect of radiofrequency catheter ablation on cardiac function and quality of life in atrial fibrillation patients.

Objective: To conduct a comprehensive analysis of the therapeutic efficacy of radiofrequency ablation (RFA) in patients with atrial fibrillation (AF), focusing on the effect on cardiac function and quality of life.

Methods: Ninety-eight AF patients treated at the Second Affiliated Hospital of Xi'an Jiaotong University were included in this study. Of these, 45 patients were assigned to the control group, which only underwent mitral valve replacement surgery, while 53 patients in the observation group that underwent RFA in addition to mitral valve replacement.

Hypercalcemia in children with APL caused by interactions between voriconazole and all-trans retinoic acid: A case report and literature review.

Rationale: All-trans retinoic acid (ATRA) and voriconazole (VRZ) are pivotal drugs for the treatment of acute promyelocytic leukemia (APL) and invasive fungal infections, respectively. When ATRA is co-administered with VRZ, clinically significant drug interactions may occur due to alterations in drug metabolism and clearance.

Patient Concerns: We report a case of hypercalcemia caused by the interaction between ATRA and VRZ in a child with APL.

Targeting and structural engineering of light-responsive nanoprobes for hierarchical therapy: construction, optimization, and applications in cancer stem cells.

Cancer stem cells (CSCs) possess the ability to self-renew and exhibit high differentiation potential, and they have been proven to be responsible for the maintenance, growth, and metastasis of tumors. As such, accurate identification and targeted therapy for CSCs are of great importance in clinical treatment. Here, a dual-targeted and light-responsive nanoprobe is presented, utilizing the reconstructed mesoporous SiO of a binary fatty acid eutectic mixture and a gold porous shell.

Role of dexmedetomidine in postoperative cognitive dysfunction and sleep improvement in aged rats by regulating the PI3K/Akt signaling pathway and its mechanism.

Objective: This study aims to explore the mechanism of dexmedetomidine (Dex) in improving postoperative cognitive dysfunction (POCD) and postoperative sleep in aged rats through the PI3K/Akt signaling pathway.

Methods: Splenectomy was used to establish a POCD model in aged rats. Open field test (OFT) and new object recognition test (NORT) were used to observe the cognitive function of rats The awakening and sleep times of rats were recorded.

Hypofractionated partial breast irradiation after breast-conserving surgery for patients with early stage breast cancer in China Mainland: a single-arm prospective trial.

To report the results of a single-arm, prospective partial breast irradiation (PBI) trial from China mainland using a dose of 40.05 Gy in 15 fractions delivered with intensity-modulated radiation therapy (IMRT) technique for patients with early stage breast cancer. Patients aged ≥ 50 years who underwent breast-conserving surgery for unifocal non-lobular invasive breast cancer, with pathological T1 disease, clear margins, negative axillary nodes, and positive hormonal receptors, were recruited.

Patients' loyalty to primary care institutions and associated factors in China: a cross-sectional study.

Objectives: This study aimed to examine patients' loyalty and explore some associated factors among PCIs in this country.

Methods: A multistage stratified random sampling method was employed to collect data for this study. A structured self-administered questionnaire was distributed to 2,697 patients who had ever involved in seeking healthcare from PCIs across China between July and September in 2021.

Value of diaphragmatic ultrasound parameters in assessing weaning outcomes and survival in ventilator-dependent intensive care unit patients.

Objective: To retrospectively evaluate the utility of diaphragmatic ultrasound parameters in assessing ventilator weaning outcomes and survival in ventilator-dependent intensive care unit (ICU) patients.

Methods: A total of 105 ventilator-dependent ICU patients admitted to our hospital between October 2019 and February 2024 were included in this study. Depending on weaning outcomes, patients were divided into a successful group (n = 86) and a failure group (n = 19).

Genome-wide identification and functional roles relating to anthocyanin biosynthesis analysis in maize.

Background: Anthocyanin is an important class of water-soluble pigments that are widely distributed in various tissues of plants, and it not only facilitates diverse color changes but also plays important roles in various biological processes. Maize silk, serving as an important reproductive organ and displaying a diverse range of colors, plays an indispensable role in biotic resistance through its possession of anthocyanin. However, the copy numbers, characteristics, and expression patterns of genes involved in maize anthocyanin biosynthesis are not fully understood.

Sibanin, a novel black fly-derived Kunitz protease inhibitor, prevents thrombus formation in mice by anticoagulation-antiplatelet duality.

Most Kunitz inhibitors exhibit serine protease inhibitory activity, but limited information is available on the regulation of platelet function. Herein, we report the purification and characterization of a novel single Kunitz domain inhibitor (Sibanin) from the salivary glands of the black fly Simulium bannaense. Recombinant Sibanin prolonged activated partial thromboplastin time and prothrombin time, and exhibited high-affinity binding to FXa and elastase with a KD of 5.

Insight into the effect of ZIF-8 on the interaction between drugs and protein/cell.

Understanding the impact of nanomaterials on drug-protein/cell interactions is crucial for comprehending their in vivo biological effects. We investigated the impact of zeolitic imidazolate framework (ZIF)-8 on the interaction between curcumin (Cur) and human serum albumin (HSA) using various spectroscopic techniques and molecular docking. Additionally, we examined its effect on drug-cell interaction using HepG2 cells and Escherichia coli (E.

Dual Inhibition of CDK4/6 and CDK7 Suppresses Triple-Negative Breast Cancer Progression via Epigenetic Modulation of SREBP1-Regulated Cholesterol Metabolism.

Inhibitors targeting cyclin-dependent kinases 4 and 6 (CDK4/6) to block cell cycle progression have been effective in treating hormone receptor-positive breast cancer, but triple-negative breast cancer (TNBC) remains largely resistant, limiting their clinical applicability. The study reveals that transcription regulator cyclin-dependent kinase7 (CDK7) is a promising target to circumvent TNBC's inherent resistance to CDK4/6 inhibitors. Combining CDK4/6 and CDK7 inhibitors significantly enhances therapeutic effectiveness, leading to a marked decrease in cholesterol biosynthesis within cells.

ZSCAN16 expedites hepatocellular carcinoma progression via activating TBC1D31.

Objective: Hepatocellular carcinoma (HCC) is fatal and poses great challenges to early diagnosis and effective treatment. This paper sought to expound the function of Zinc finger and SCAN domain-containing protein 16 (ZSCAN16) and TBC1 domain family member 31 (TBC1D31) in HCC progression.

Methods: ZSCAN16 and TBC1D31 levels were detected by RT-qPCR, Western blot, and immunohistochemistry.

[Clinical features and prognosis of children with fungal bloodstream infection following chemotherapy for acute leukemia].

Objectives: To investigate the clinical features and prognosis of children with fungal bloodstream infection (BSI) following chemotherapy for acute leukemia (AL).

Methods: A retrospective analysis was performed on 23 children with fungal BSI following chemotherapy for AL in three hospitals in Fujian Province, China, from January 2015 to December 2023. Their clinical features and prognosis were analyzed.

Clinical outcomes after post-operative radiotherapy for breast cancer patients presenting with ipsilateral supraclavicular metastasis: considerations on the cranial border of irradiation field.

Background: Disease recurrence at lower neck adjacent to ipsilateral supraclavicular (SCV) region represents a concern in locally advanced breast cancer patients presenting with SCV metastasis at diagnosis. This study aims to report the outcomes following post-operative radical radiation therapy and discuss the reasonable cranial border of the irradiation field for N3c patients.

Methods: Between July 2016 and January 2022, a total of 268 patients were eligible for analysis.

Single-Dose Drug Development Candidate for Schistosomiasis.

Aryl hydantoins were identified in the early 1980s as a promising antischistosomal chemotype. However, as exemplified by Ro 13-3978, this compound series produced antiandrogenic side effects on the host, a not unexpected outcome given their structural similarity to the antiandrogenic drug nilutamide. The two key advances in our optimization of Ro 13-3978 were swapping the aryl trifluoromethyl substituent with a difluoroethyl to abolish antiandrogenic effects and replacing the hydrogen atoms of the -dimethyl substructure with deuterium atoms to increase metabolic stability.