Mapping the full vascular network in the mouse brain at submicrometer resolution.

Mapping the microvascular networks in the brain can lead to significant scientific and clinical insights. We developed a serial sectioning microscopy technique called the Knife-Edge Scanning Microscopy (KESM) to section and image the entire mouse brain at submicrometer resolution. In our effort to map the entire vascular network in the mouse brain, we perfused the vessels with India ink, and used KESM to image the prepared brain.