Publications by authors named "Widodo"

Laccases, multi-copper oxidases, play pivotal roles in the oxidation of a variety of substrates, impacting numerous biological functions and industrial processes. However, their industrial adoption has been limited by challenges in thermostability. This study employed advanced computational models, including random forest (RF) regressors and convolutional neural networks (CNNs), to predict and enhance the thermostability of laccases.

View Article and Find Full Text PDF

Testicular tumors are the most common malignancy of young men and tumors affecting the testis are caused by somatic mutations in germ or germ-like cells. The PI3K pathway is constitutively activated in about one third of testicular cancers. To investigate the role of the PI3K pathway in transforming stem-like cells in the testis, we investigated tumors derived from mice with post-natal, constitutive activation of PI3K signaling and homozygous deletion of tumor suppressor Pten, targeted to nestin expressing cells.

View Article and Find Full Text PDF

This systematic review examines the role of religious leaders in adolescents' reproductive health and family planning. Following PRISMA guidelines, databases like PubMed, ScienceDirect, Scopus, ProQuest, and Web of Science were searched, yielding 1435 articles from 2018 to 2023. Inclusion criteria comprised of open access original peer-reviewed articles that were written in English language, with a concentration on adolescent health.

View Article and Find Full Text PDF
Article Synopsis
  • * The study focused on improving the treatment of MBD of unknown origin by implementing the INA-MBD algorithm, aiming to streamline the diagnostic process and reduce costs.
  • * A quasi-experimental study was conducted with 128 patients divided into intervention and non-intervention groups, using various analysis methods to assess the effectiveness of the INA-MBD algorithm in terms of time and costs associated with diagnosing MBD.
View Article and Find Full Text PDF
Article Synopsis
  • Myelomeningocele (MMC) primarily impacts low-resource regions, especially where there is no mandatory folic acid fortification, but research on its neurosurgical management is concentrated in high-income countries (HICs).
  • A systematic review revealed that while HICs account for a small percentage of global neural tube defect cases, they dominate both authorship and patient representation in the research literature, with minimal contributions from lower-income countries.
  • Findings suggest a significant disparity in MMC research output relative to disease burden, highlighting an urgent need for increased scholarly attention and resource allocation in lower-income countries where MMC cases are more prevalent.
View Article and Find Full Text PDF
Article Synopsis
  • * Three drying techniques (sun drying, oven drying, and freeze drying) were tested, showing that dried E. cottonii significantly decreased methane production while improving dry matter (DM) and organic matter (OM) degradability.
  • * All three drying methods had similar effects on methane reduction, but freeze drying proved to be the most effective method without negatively affecting rumen bacterial activities or total volatile fatty acid (VFA) production.
View Article and Find Full Text PDF

The livestock-associated methicillin-resistant (LA-MRSA) strains are prevalent in the poultry farming environment and are a common component of the bacterial microbiota on the skin and mucous membranes of healthy animals. The origin and spread of LA-MRSA are attributed to the use of antibiotics in animals, and close contact between people and different animal species increases the risk of animal exposure to humans. The epidemiology of LA-MRSA in poultry significantly changed when ST398 and ST9 were found in food-producing animals.

View Article and Find Full Text PDF

Using ergonomic orthopaedic chairs can address spinal issues, particularly HNP, due to their flexible and elastic properties. By achieving optimal ergonomic design, various positive aspects can be achieved, such as increasing work quality productivity and reducing the cost of injuries or accidents. This research demonstrates diverse product design approaches to enhance posture health ergonomics, address specific musculoskeletal disorder issues, and consider local needs and available materials.

View Article and Find Full Text PDF

Aim: To evaluate the essential role of Moringa-albumin (MA) formulation in the maintenance of complement regulatory proteins through CD55 and CD59 on erythrocyte (TER-119).

Methods: Streptozotocin (145 mg/kg) was used to induce diabetes mice. In diabetes mellitus (DM) model mice were treated with MA formulation for 14 days at a D1 - 500 mg/kg (M) + 620 mg/kg BW (A), D2 - 1000 mg/kg (M) + 420 mg/kg (A), and D3 - 1500 mg/kg (M) + 200 mg/kg (A).

View Article and Find Full Text PDF

Anti-Müllerian hormone (AMH) is a marker for predicting embryo production in cows subjected to superovulation; however, it remains to be established as a reliable predictor of reproductive performance. We hypothesized that the serum AMH concentration of donors affects the ovarian response to follicle-stimulating hormone (FSH) treatment during superovulation. Herein, we retrospectively investigated the association between different FSH doses and AMH concentrations in donor Japanese Black cows in a superovulation program and analyzed the number of total and transferable embryos recovered.

View Article and Find Full Text PDF

Extended-spectrum β-lactamases (ESBLs) are β-lactamase enzymes produced by Gram-negative bacterial pathogens that harbor the ESBL genes. In addition, most ESBL genes are plasmid-mediated and usually encode a broader spectrum of antimicrobial resistance, especially to penicillins, first-generation, second-generation, and third-generation cephalosporins, as well as monobactam, such as aztreonam. has become an opportunistic pathogen, especially in poultry, and has been implicated in zoonotic diseases that can be transmitted to humans, resulting in public health problems.

View Article and Find Full Text PDF

Objective: This study aims to determine the effect of adding saponified black soldier fly larvae oil calcium salt (BSFLO-SCa) to quail feed as an alternative source of fat on laying performance, blood lipid profile, egg quality, and gene expression in lipid metabolism.

Methods: A total of 120 female Japanese quails (Coturnix japonica) aged 24 weeks were divided into 3 treatments, each with 8 replications, and each replication consisted of 5 quails in a completely randomized design. The applied treatments were the inclusion of basal feed as a control (T0) and basal feed supplemented with 1% BSFLO-SCa (T1) and 2% BSFLO-SCa (T2).

View Article and Find Full Text PDF

This study investigated the effect of substances on nonspecific immune responses of head kidney leukocytes, the antimicrobial activity against sp., as well as the time-kill of sp. by combining the substances with lactic acid bacteria (LAB) and sp.

View Article and Find Full Text PDF

Introduction: Fast melt tablets (FMTs) provide a convenient dosage form that rapidly dissolves on the tongue without the need for water. Cocoa butter serves as a suitable matrix system for FMTs formulation, facilitating rapid disintegration at body temperature.

Objectives: This study aimed to formulate FMTs using cocoa butter as a base and investigate the effect of various disintegrants and superdisintegrants on their characteristics.

View Article and Find Full Text PDF

Introduction: While acute and chronic subdural hematomas (SDH) are relatively common, co-occurrence is rare. Here, we report a case of unilateral simultaneous acute and chronic SDH.

Case Presentation: A 74-year-old man with comorbid diabetes mellitus and hypertension presented with decreased consciousness (Glasgow Coma Scale (GCS) 4; E1M2V1) with stable hemodynamics.

View Article and Find Full Text PDF
Article Synopsis
  • This study investigates the effectiveness of intercostal nerve transfer combined with either umbilical cord-derived mesenchymal stem cells (UC-MSC) or secretome therapy in patients with total brachial plexus injury.
  • Using a randomized controlled method, the research evaluates pre- and post-operative outcomes in muscle strength, quality of life, and histological assessments in 15 patients over 8 months.
  • Results indicate significant functional improvements across both treatment groups, with the UC-MSC group showing better health changes, though no notable differences in histological recovery measures were observed.
View Article and Find Full Text PDF

The widespread presence of Fusarium mycotoxins in animal feed is a global issue, not only for the health of livestock but also for ensure the safety of food as an end product. High concentrations of zearalenone (ZEN) and deoxynivalenol (DON) have been detected in the diets of Japanese Black (JB) and Holstein Friesian (HF) breeding herds. Consequently, we monitored serum biochemical parameters over a long time in both herds, focusing on anti-Müllerian hormone (AMH) levels and acute-phase inflammation.

View Article and Find Full Text PDF
Article Synopsis
  • * A case of a 24-year-old male post-motorcycle accident showed persistent eye and facial issues, which were addressed through a second surgery guided by a 3D-printed model for facial reconstruction.
  • * The use of 3D printing in facial surgery enables precise planning and design, improving outcomes and aiding in the reconstruction process with materials like rib cartilage.
View Article and Find Full Text PDF
Article Synopsis
  • Neuregulin-1 (NRG1) is important for the growth and development of various tissues, including the gut, brain, and muscles, and has multiple protein isoforms.
  • A new class of NRG1 called NRG1-VII was identified, which has unique transcripts and structural differences compared to other isoforms, specifically originating from myeloid cells.
  • NRG1-VII is mainly expressed in monocytes and macrophages, suggesting it may play a significant role in tissue repair, though its specific functions remain unclear.
View Article and Find Full Text PDF

Background: Nurses play a critical role in combating the COVID-19 pandemic, facing numerous challenges in providing care to COVID-19 patients, particularly addressing their psychological issues. This study aims to explore nurses' experiences in overcoming care challenges and enhancing nursing strategies. The study was held in Indonesia.

View Article and Find Full Text PDF

Background And Aim: The intricate healing process involves distinct sequential and overlapping phases in thermal injury. To maintain the zone of stasis in Jackson's burn wound model, proper wound intervention is essential. The extent of research on the histoarchitecture of thermal wound healing and the application of mesenchymal stem cell (MSC)-free-based therapy is limited.

View Article and Find Full Text PDF

Background And Aim: Toxoplasmosis is caused by the parasite . Cats are the only known hosts that excrete resistant oocysts. Wild rats serve as crucial reservoirs and intermediate hosts for survival and dissemination.

View Article and Find Full Text PDF

This meta-analysis consolidates various related studies to identify patterns in the impact of feeding algae on performance aspects, including milk fat, milk protein, and carcass yield in several ruminant species, such as cattle, sheep, and goats. The data were collected from 67 articles that examined factors such as the type of algae (macro- and microalgae), algal species, and animal breed. Barki sheep, Moghani sheep, and Zaraibi goats demonstrated an increased average daily gain (P < 0.

View Article and Find Full Text PDF

Introduction: Breeding for oil palm resistance against basal stem rot caused by Ganoderma boninense is challenging and time-consuming. Advanced oil palm gene pools are very limited, hence it is assumed that parental palms have experienced genetic drift and lost their resistance genes against Ganoderma. High-throughput selection criteria should be developed.

View Article and Find Full Text PDF

is known for its capacity to regulate blood glucose levels in individuals with diabetes, while leaves have a traditional history as an alternative therapy due to their antidiabetic compounds. The combination of these two plants is expected to yield more optimized antidiabetic agents. This study aims to assess the antidiabetic activity of the combined ethanolic extract of and leaves by measuring the inhibition of the -glucosidase enzyme and the blood glucose level in streptozotocin-induced rats and to determine the phytochemical contents of total phenolics, total flavonoids, and quercetine as marker compounds.

View Article and Find Full Text PDF