Differential Effects on HbA1c Detection by HPLC and Capillary Electrophoresis in Five Types of Hb Variants in China.

Objective: Hemoglobin A1c (HbA1c) can be used to evaluate blood glucose control. Its measurement will be affected by many factors, but Hb variation is the most critical factor. This study aimed to explore the types of variants found in routine work and their impact on test results.

Evaluation of the Diagnostic Value of FliD, a Helicobacter pylori Flagellar Protein.

Novel immunodominant antigens are urgently required for the diagnosis and vaccination of Helicobacter pylori (HP). FliD, an important colonization factor, was cloned and expressed (rFliD) to evaluate the levels of specific immunoglobulin G (IgG), IgM, and IgA antibodies in the serum of patients using ELISA. Rabbit anti-rFliD polyclonal antibody (pAb) was obtained by the subcutaneous injection of rFliD.

A Highly Sensitive Electrochemical Glucose Sensor Based on Room Temperature Exfoliated Graphite-Derived Film Decorated with Dendritic Copper.

An ultrasensitive enzyme-free glucose sensor was facilely prepared by electrodepositing three-dimensional dendritic Cu on a room temperature exfoliated graphite-derived film (RTEG-F). An excellent electrocatalytic performance was demonstrated for glucose by using Cu/RTEG-F as an electrode. In terms of the high conductivity of RTEG-F and the good catalytic activity of the dendritic Cu structures, the sensor demonstrates high sensitivities of 23.

A self-attention based neural architecture for Chinese medical named entity recognition.

The combination of medical field and big data has led to an explosive growth in the volume of electronic medical records (EMRs), in which the information contained has guiding significance for diagnosis. And how to extract these information from EMRs has become a hot research topic. In this paper, we propose an ELMo-ET-CRF model based approach to extract medical named entity from Chinese electronic medical records (CEMRs).

A Pitfall in HbA1c Testing Caused by Hb Long Island Hemoglobin Variant.

Background: Although many factors may interfere with hemoglobin (Hb)A1c measurement, Hb variants are among the most important factors.

Methods: We tested the HbA1c levels of the patient, a 32 year old Manchu Chinese woman, during a routine health check. We used different methods, including high-performance liquid chromatography (HPLC) and capillary electrophoresis, to test specimens from the patient.

Enhancement of butanol production in Clostridium acetobutylicum SE25 through accelerating phase shift by different phases pH regulation from cassava flour.

A prominent delay with 12h was encountered in the phase shift from acidogenesis to solventogenesis in butanol production when the substrate-glucose was replaced by cassava flour. To solve this problem, different phase of pH regulation strategies were performed to shorten this delay time. With this effort, the phase shift occurred smoothly and the fermentation time was shortened.

Treatment of Intestinal Paralysis Using Revised Major Purgative Decoction Combined with Nasointestinal Decompression: Experience with 31 Patients.

To investigate the feasibility of using a revised major purgative decoction in combination with nasointestinal decompression for the treatment of intestinal paralysis. 31 patients with intestinal paralysis underwent gastrointestinal decompression. A fluoroscopic guided tri-lumen nasointestinal decompression tube was placed, and the patients were randomly divided into two groups: patients in the study group (n = 16) received 100 ml of a revised major purgative decoction infused through the decompression tube, three times daily; and patients in the control group (n = 15) were given neostigmine 0.

An intratracheal challenge murine model of asthma: can bronchial inflammation affect the nose?

Purpose: Extensive data support the influence of the upper airway on lower airway inflammation and pathophysiology in allergic disease. However, few studies have focused on allergic inflammation in the nose after an isolated lower airway allergen challenge, a situation that can exist clinically when human subjects breathe primarily through the mouth, as occurs when nasally congested. This study used a mouse model to investigate whether upper airway inflammation and hyperresponsiveness were induced by an isolated lower airway allergen challenge.

Blocking HIF-1α following radiotherapy to prolong and enhance the immune effects of radiotherapy: a hypothesis.

Tumor local immune escape is one of the "hallmarks" of cancer leading to poor prognosis. The effects of local radiotherapy on tumors are rapidly emerging as opportunities to remodel and enhance immunity against cancer. However, this immunity remodeling and enhancing are not permanent after local radiotherapy.

Control of ultra-intense single attosecond pulse generation in laser-driven overdense plasmas.

Ultra-intense single attosecond pulse (AP) can be obtained from circularly polarized (CP) laser interacting with overdense plasma. High harmonics are naturally generated in the reflected laser pulses due to the laser-induced one-time drastic oscillation of the plasma boundary. Using two-dimensional (2D) planar particle-in-cell (PIC) simulations and analytical model, we show that multi-dimensional effects have great influence on the generation of AP.

Health risks associated with heavy metals in the drinking water of Swat, northern Pakistan.

The concentrations of heavy metals such as Cd, Cr, Cu, Mn, Ni, Pb and Zn were investigated in drinking water sources (surface and groundwater) collected from Swat valley, Khyber Pakhtunkhwa, Pakistan. The potential health risks of heavy metals to the local population and their possible source apportionment were also studied. Heavy metal concentrations were analysed using atomic absorption spectrometer and compared with permissible limits set by Pakistan Environmental Protection Agency and World Health Organization.

Heavy metals in agricultural soils and crops and their health risks in Swat District, northern Pakistan.

This study assessed the concentrations of heavy metals such as cadmium (Cd), chromium (Cr), copper (Cu), manganese (Mn), nickel (Ni) and zinc (Zn) in agricultural soils and crops (fruits, grains and vegetable) and their possible human health risk in Swat District, northern Pakistan. Cd concentration was found higher than the limit (0.05 mg/kg) set by world health organization in 95% fruit and 100% vegetable samples.

[Study on expression of Treg cells in attenuated Schistosoma japonicum cercariae immunized BALB/c mice].

Objective: To study the expression differences of CD4+CD25+Foxp3+Treg cells between the attenuated cercariae immunized mice and the normal infected mice and discuss the immune protection mechanisms of the mice immunized with attenuated cercariae.

Methods: Forty female BALB/c mice were divided into 2 groups, group A, the attenuated cercariae immunized group (16 mices) and the group B, the normal cercariae infected group (16 mices), and the last 8 ones served as the blank control. The spleen cells and the ratios of PBMC's CD4+CD25+Foxp3+/CD4+CD25+T cells were compared between the attenuated cercariae immunized mice and normal mice injected by FCM and the Foxp3 expression levels in spleens and livers were assayed by IHC.