Impact of pediatric COVID-19 isolation on children's well-being and caregiver mental health.

Background: The Korean government implemented mandatory hospital isolation in the early phase of the COVID-19 pandemic. This study investigated the mental health of children and caregivers who underwent mandatory hospital isolation due to COVID-19.

Methods: This prospective study examined the physical condition and mental health of children under 7 years of age with COVID-19 and the mental health of their caregivers who underwent isolation in negative pressure rooms at two hospitals in Korea from April to September 2021.

The influence of simulated worn shoe and foot inversion on heel internal biomechanics during running impact: A subject-specific finite element analysis.

This study explored how systematic changes in running shoe degradation and foot inversion alter the distribution and peak value of heel pressure and calcaneus stress, as well as the total stress-concentration exposure (TSCE) within the calcaneal bone. A foot-shoe finite element model was employed and three shoe wear conditions (new shoe (CON), moderate worn shoe (MWSC), excessive worn shoe (EWSC)) coupled with three foot inversion angles (0°, 10°, 20°) were further modulated. Simulations were conducted at the impact peak instant during running.

Chimeric antigen receptor with novel intracellular modules improves antitumor performance of T cells.

The excessive cytokine release and limited persistence represent major challenges for chimeric antigen receptor T (CAR-T) cell therapy in diverse tumors. Conventional CARs employ an intracellular domain (ICD) from the ζ subunit of CD3 as a signaling module, and it is largely unknown how alternative CD3 chains potentially contribute to CAR design. Here, we obtained a series of CAR-T cells against HER2 and mesothelin using a domain comprising a single immunoreceptor tyrosine-based activation motif from different CD3 subunits as the ICD of CARs.

Propensity Score Matching Analysis for Alcohol-Related Liver Disease.

Objectives: This study aims to investigate the impact of comorbidity with chronic hepatitis B (CHB) on the survival rates and incidence of liver cancer in patients with alcohol-related liver disease (ARLD).

Methods: Patients with ARLD and those with ARLD co-morbid with CHB were included in this study and designated as the ARLD group and the ARLD + HBV group, respectively. Propensity score matching (PSM) was then employed to compare survival rates and liver cancer development between these two groups.

Variations in Revascularization Strategies for Chronic Limb-Threatening Ischemia: A Nationwide Analysis of Medicare Beneficiaries.

Background: Recent data support both surgical-first and endovascular-first revascularization approaches for chronic limb-threatening ischemia (CLTI), but hospital-based practices are poorly described.

Objectives: This aim of this study was to characterize contemporary variations and outcomes associated with each strategy among U.S.

GmARF15 Enhances the Resistance of Soybean to by Promoting Expression in Response to Salicylic Acid Signalling.

Phytophthora root and stem rot caused by () is a globally prevalent oomycete disease. The use of resistant cultivars is an effective and environmentally friendly strategy to manage this disease. It is important to understand the molecular mechanisms underlying the response of (soybean) to infection.

Improvement of plant growth and fruit quality by introducing a phosphoribosylpyrophosphate synthetase mutation into Methylorubrum populi.

Aims: The aim of this study was to evaluate the impact of the introduction of a phosphoribosylpyrophosphate synthetase (PRS) mutation into a plant growth-promoting strain of Methylorubrum on the enhancement of phyllosphere colonization, with the ultimate goal of improving plant growth and quality.

Methods And Results: A strain of Methylorubrum populi (named HS04) was isolated from the groundnut leave and found to process the plant-promoting traits, including the ability to produce indole acetic acid, siderophore, 1-aminocyclopropane-1- carboxylate deaminase, and to fix nitrogen. The application via foliar spray significantly increased the fresh weight of cucumber seedlings cultivated in a standard growth chamber, with 43.

Contemporary Administrative Codes to Identify Pulmonary Vein Isolation Procedures for Atrial Fibrillation.

Background: Use of pulmonary vein isolation (PVI) to treat atrial fibrillation continues to increase. Despite great interest in leveraging administrative data for real-world analyses, contemporary procedural codes for identifying PVI have not been evaluated.

Methods And Results: In this observational retrospective cohort study, inpatient PVIs were identified among US Medicare fee-for-service beneficiaries using Current Procedural Terminology (CPT) code 93656 in Carrier Line Files.

Overlooked and underpowered: a meta-research addressing sample size in radiomics prediction models for binary outcomes.

Objectives: To investigate how studies determine the sample size when developing radiomics prediction models for binary outcomes, and whether the sample size meets the estimates obtained by using established criteria.

Methods: We identified radiomics studies that were published from 01 January 2023 to 31 December 2023 in seven leading peer-reviewed radiological journals. We reviewed the sample size justification methods, and actual sample size used.

Knee position affects medial gastrocnemius and soleus activation during dynamic plantarflexion: no evidence for an inter-muscle compensation in healthy young adults.

Knee joint position influences ankle torque, but it is unclear whether the soleus compensates to counteract the reductions in gastrocnemius output during knee-flexed versus knee-extended plantarflexions. Therefore, the purpose of this study was to determine the effects of knee joint position and plantarflexion contraction velocity on ankle plantarflexion torque and electromyography activity of the medial gastrocnemius and soleus in healthy young adults. Healthy male participants (n=30) performed concentric plantar flexions in a custom-built dynamometer from 15° dorsiflexion to 30° plantarflexion at gradually increasing velocities during each contraction at 30, 60, 120, 180, and 210° s-1 in a supine position with the knee fully extended and while kneeling with the knee fixed in 90° flexion.

Intestinal TM6SF2 protects against metabolic dysfunction-associated steatohepatitis through the gut-liver axis.

Transmembrane-6 superfamily member 2 (TM6SF2) regulates hepatic fat metabolism and is associated with metabolic dysfunction-associated steatohepatitis (MASH). TM6SF2 genetic variants are associated with steatotic liver disease. The pathogenesis of MASH involves genetic factors and gut microbiota alteration, yet the role of host-microbe interactions in MASH development remains unclear.

Insights into the regulation mechanisms of dual hydrothermal treatment on the structure and digestive characteristics of A- and B-type wheat starch granules.

Hydrothermal treatment is a physical modification technology to alter starch structures for the production of resistant starch (RS). However, the underlying regulation mechanism of the multiscale structure and digestive properties of starch by dual hydrothermal synergistic treatment remains unclear. To solve this problem, A- and B-type wheat starch granules (AWS and BWS) were separated and subjected to toughening and heat-moisture synergistic treatment (THT) with various moisture content (10 %, 15 %, 20 %, 25 %).

Integrating multi-omics features enables non-invasive early diagnosis and treatment response prediction of diffuse large B-cell lymphoma.

Background: Multi-omics features of cell-free DNA (cfDNA) can effectively improve the performance of non-invasive early diagnosis and prognosis of cancer. However, multimodal characterization of cfDNA remains technically challenging.

Methods: We developed a comprehensive multi-omics solution (COMOS) to specifically obtain an extensive fragmentomics landscape, presented by breakpoint characteristics of nucleosomes, CpG islands, DNase clusters and enhancers, besides typical methylation, copy number alteration of cfDNA.

Ginkgo biloba extract mediates HT22 cell proliferation and migration after oxygen-glucose deprivation/reoxygenation via regulating RhoA-ROCK2 signalling pathway.

Vascular dementia (VD) is a neurocognitive disorder resulting from cerebral vascular disorders, leading to the demise of neurons and cognitive deficits, posing significant health concerns globally. Derived from Ginkgo biloba leaves, EGb761 is a potent bioactive compound widely recognized for its benefits in treating cerebrovascular diseases. Previous studies have demonstrated that the administration of EGb761 to VD rats enhances the proliferation, differentiation, and migration of neurons, effectively alleviating cognitive dysfunction.

Exploring lipidome mediated inflammatory pathways in acute pancreatitis using mendelian randomization.

Acute pancreatitis (AP) is a severe gastrointestinal condition with an increasing incidence of hyperlipidemic etiology. The investigation employed a two-sample, bidirectional Mendelian randomization method to investigate potential causal relationship between lipidome profiles, inflammatory mediators, and AP. Exploration of genetic variants across the genome in a study population of 10,630 AP cases and 844,679 non-AP individuals revealed multiple lipidome entities significantly associated with AP risk.

Disease Burden Estimation of Hepatocellular Carcinoma Attributable to Dietary Aflatoxin Exposure in Sichuan Province, China.

Background: Aflatoxin B (AFB), AFB, AFG, and AFG are Group 1 human carcinogens, with AFB notably increasing hepatocellular carcinoma (HCC) risk. Sichuan Province, China, with its subtropical monsoon climate, is susceptible to AF contamination in various food items. However, the HCC disease burden attributable to lifetime chronic dietary AF intake in Sichuan has not been investigated.

Ref-1 is overexpressed in neovascular eye disease and targetable with a novel inhibitor.

Reduction-oxidation factor-1 or apurinic/apyrimidinic endonuclease 1 (Ref-1/APE1) is a crucial redox-sensitive activator of transcription factors such as NF-κB, HIF-1α, STAT-3 and others. It could contribute to key features of ocular neovascularization including inflammation and angiogenesis; these underlie diseases like neovascular age-related macular degeneration (nAMD). We previously revealed a role for Ref-1 in the growth of ocular endothelial cells and in choroidal neovascularization (CNV).

Oxidized hyaluronic acid/ε-polylysine/quaternized chitosan hydrogel with shear thinning injectability and self-healing properties for full-time and multipurpose wound healing.

Complex wound closure scenarios necessitate the development of advanced wound dressings that can effectively address the challenges of filling irregularly shaped wounds and managing fatigue failures encountered in daily patient activities. To tackle these issues, we develop a multifunctional hydrogel from natural polysaccharides and polypeptides with injectability and self-healing properties for promoting full-time and multipurpose wound healing. Synthesized through dynamic Schiff base linkages between oxidized hyaluronic acid (OHA), ε-polylysine (ε-PL), and quaternized chitosan (QCS), the OHA/ε-PL/QCS hydrogel can gel rapidly within 50 s.

Molecular mechanisms of libido influencing semen quality in geese through the hypothalamic-pituitary-testicular-external genitalia axis.

Libido plays a crucial role in influencing semen quality, yet the underlying regulatory mechanisms remain unclear. As a central axis in male goose reproduction, the hypothalamic-pituitary-testicular-external genitalia (HPTE) axis may contribute to the regulation of this process. In this study, we established a rating scale for goose libido based on average number of massages to erection (ANM) and the erection type, and evaluated semen quality across the entire flock.

Integrating crowdsourced data in the built environment studies: A systematic review.

The integration of crowdsourced data has become central to contemporary built environment studies, driven by the rapid growth in digital technologies and participatory approaches that characterize modern urbanism. Despite its potential, a systematic framework for its analysis remains underdeveloped. This review, conducted in accordance with the PRISMA protocol, examines the use of crowdsourced data in shaping the built environment, scrutinizing its applications, crowdsourcing techniques, methodologies, and comparison with other big data forms.

Ultrafast Synthesis of Oxygen Vacancy-Rich MgFeSiO Cathode to Boost Diffusion Kinetics for Rechargeable Magnesium-Ion Batteries.

Rechargeable magnesium ion batteries (RMBs) have drawn extensive attention due to their high theoretical volumetric capacity and low safety hazards. However, divalent Mg ions suffer sluggish mobility in cathodes owing to the high charge density and slow insertion/extraction kinetics. Herein, it is shown that an ultrafast nonequilibrium high-temperature shock (HTS) method with a high heating/quenching rate can instantly introduce oxygen vacancies into the olivine-structured MgFeSiO cathode (MgFeSiO-HTS) in seconds.

CircRNA-loaded DC vaccine in combination with low-dose gemcitabine induced potent anti-tumor immunity in pancreatic cancer model.

Although promising, dendritic cell (DC) vaccines may not suffice to fully inhibit tumor progression alone, mainly due to the short expression time of the antigen in DC vaccines, immunosuppressive tumor microenvironment, and tumor antigenic modulation. Overcoming the limitations of DC vaccines is expected to further enhance their anti-tumor effects. In this study, we constructed a circRNA-loaded DC vaccine utilizing the inherent stability of circular RNA to enhance the expression level and duration of the antigen within the DC vaccine.

The analysis on forest farmers' intention to participate in construction of the National Reserve Forest Project and its driving factors.

The construction of the National Reserve Forest Project is the fundamental measure to alleviate the contradiction between national wood supply and demand and maintain national ecological security and wood security. This study analyzed the forest farmers' intention to participate in the project and its driving factors. It provides a scientific basis for mobilizing forest farmers' enthusiasm for continuous participation, improving their sense of gain, and realizing the established goals of constructing the National Reserve Forest Project.