Letter to the editor regarding: "Tirofiban vs. aspirin in patients with acute ischemic stroke: A meta-analysis of randomized clinical trials".

The article by De Oliveira et al. presents an insightful systematic review and meta analysis comparing intravenous tirofiban and oral aspirin in acute ischemic stroke (AIS) patients. It has sparked substantial discussions among my colleagues and me, particularly regarding the optimal circumstances for administering intravenous tirofiban in AIS patients ineligible for reperfusion therapies.

Mediating role of organized sports participation on the relationship between body fatness and arterial wall thickness among adolescents: ABCD Growth Study.

Background: Participation in sports is the main manifestation/subdomain of physical exercise in adolescents and affects cardiovascular health in different ways.

Objectives: To analyze the relationship between body fatness and arterial wall thickness as well as the mediating role of sports participation in this relationship among adolescents.

Methods: This cross-sectional study is linked to the ABCD-Growth Study conducted in Presidente Prudente, Brazil.

Time-course RNA-seq analysis of upland cotton (Gossypium hirsutum L.) responses to Southern root-knot nematode (Meloidogyne incognita) during compatible and incompatible interactions.

Background: The Southern root-knot nematode (Meloidogyne incognita) poses a substantial threat to cotton (Gossypium hirsutum L.) by causing significant agricultural losses. Host plant resistance is the most plausible approach for minimizing these losses.

Potent and selective SETDB1 covalent negative allosteric modulator reduces methyltransferase activity in cells.

A promising drug target, SETDB1, is a dual methyl-lysine (Kme) reader and methyltransferase implicated in cancer and neurodegenerative disease progression. To help understand the role of the triple Tudor domain (3TD) of SETDB1, its Kme reader, we first identify a low micromolar potency small molecule ligand, UNC6535, which occupies simultaneously both the TD2 and TD3 reader binding sites. Further optimization leads to the discovery of UNC10013, a covalent 3TD ligand targeting Cys385 of SETDB1.

A Comparative Evaluation of Selection Processes for Helicopter Emergency Medical Service Paramedics in Australasia.

Objective: Providing transparency on the state of selection processes in helicopter emergency medical services (HEMS) across different services is a vital step in developing universal standards for HEMS aircrew. This study aimed to consolidate, rate, and evaluate information on selection processes from 9 HEMS across Australia and New Zealand.

Methods: This was a descriptive study combining objective and subjective assessment of selection to HEMS teams.

Piezoelectric PVDF Nanoparticles for Enhanced Antimicrobial Activity via Mechanical Stimulation: A Proof-of-Concept Study.

The alarming rise of antimicrobial resistance is a public health issue, driven by the excessive and improper use of antibiotics, which are becoming less effective against an increasing number of microorganisms. There is an urgent need to find alternative antimicrobial strategies that can bypass bacterial resistance mechanisms. Using physical stimuli to sensitize bacteria to antimicrobial action is one step toward addressing this challenge.

Repurposing tafenoquine as a potent antifungal agent against Candida haemulonii sensu stricto.

Background: The rise in fungal infections caused by multidrug-resistant pathogens like Candida haemulonii sensu stricto presents a significant global health challenge. The common resistance to current treatments underscores the urgency to explore alternative therapeutic strategies, including drug repurposing.

Objectives: To assess the potential of repurposing tafenoquine, an antimalarial agent, for antifungal use against C.

Survey of Patients With Sanfilippo Type a (MPS IIIA) Disease Diagnosed by the MPS Brazil Network.

Mucopolysaccharidosis IIIA (MPS IIIA) is an autosomal recessive lysosomal disease caused by the deficiency of N-sulfoglucosamine sulfohydrolase (SGSH), due to biallelic mutations in the SGSH gene. We conducted a retrospective study utilizing data from MPS IIIA patients born from 1983 to 2024 diagnosed by the MPS Brazil Network. Sixty-eight patients with MPS IIIA were included.

Perception of transplant recipients and professionals about health care following kidney transplantation.

Objective: To explore the perception of renal transplant recipients and professionals about health care following kidney transplantation.

Method: Exploratory, qualitative study, carried out through focus groups and questionnaire application, in 2023, with kidney transplant recipients and healthcare professionals. The analysis was performed with the support of the software IRaMuTeQ®.

Chromosome homologies and polymorphisms in a Neotropical species complex of frogs revealed by the U2 snRNA gene.

The species complex is a Neotropical frog group that encompasses seven well-supported major clades. Although very similar morphologically, the five lineages previously karyotyped show notorious cytogenetic signatures. There is also evidence of ancient secondary contact between , which has heteromorphic sex chromosomes, and the lineage known as L1B, which lacks sex chromosome heteromorphism.

Clinical validation of an artificial intelligence algorithm for classifying tuberculosis and pulmonary findings in chest radiographs.

Background: Chest X-ray (CXR) interpretation is critical in diagnosing various lung diseases. However, physicians, not specialists, are often the first ones to read them, frequently facing challenges in accurate interpretation. Artificial Intelligence (AI) algorithms could be of great help, but using real-world data is crucial to ensure their effectiveness in diverse healthcare settings.

The Adaptation, Implementation, and Performance Evaluation of Intake24, a Digital 24-h Dietary Recall Tool for South Asian Populations: The South Asia Biobank.

Background: South Asia's diverse food supply, food preparations, and eating behaviors require dietary instruments that reflect the consumption patterns of South Asians to enable context specific dietary assessment. Such instruments are not readily available for detailed dietary assessment at scale in South Asia.

Objectives: We describe the adaptation, implementation, and performance evaluation of Intake24, an open-source digital 24-h dietary recall tool, for dietary assessment in South Asia.

Monitoring of hepatitis E virus infection and replication by functional tagging of the ORF2 protein.

Background And Aims: Hepatitis E virus (HEV) infection is a leading cause of acute hepatitis worldwide. Understanding of the mechanisms underlying productive HEV infection remains incomplete and would benefit from technological advances improving current model systems.

Methods: We exploited transposon-mediated random insertion and selection of viable clones to identify sites in the HEV open reading frame 2 (ORF2) protein, corresponding to the viral capsid, allowing for the insertion of reporter sequences in a functional context.

Stabilized dengue virus 2 envelope subunit vaccine redirects the neutralizing antibody response to all E-domains.

The four-dengue virus (DENV) serotypes cause several hundred million infections annually. Several live-attenuated tetravalent dengue vaccines (LAVs) are at different stages of clinical testing and regulatory approval. A major hurdle faced by the two leading LAVs is uneven replication of vaccine serotypes stimulating a dominant response to one serotype at the expense of the other three, leading to the potential for vaccine antibody (Ab) enhanced more severe infections by wild type DENV serotypes that fail to replicate in the vaccine.

Childhood dyslipidemia: Clinician management practices in the primary care setting.

Objectives: To describe clinician management practices for childhood dyslipidemia in the community setting.

Methods: A descriptive study was conducted for children aged 2 to 10 years with dyslipidemia as defined by the National Cholesterol Education Program Expert Panel on Cholesterol in Children criteria. A convenience sample of participants from the TARGet Kids! cohort study (Toronto, Canada) was used.

Crystallographic and Thermodynamic Evidence of Negative Coupling in the Flavin-Dependent Tryptophan Halogenases AbeH and BorH.

Flavin-dependent halogenases (FDHs) regioselectively halogenate aromatic substrates using halide ions, O, and reduced flavin (FADH) at physiological temperatures in aqueous solution, making them a green alternative to conventional synthetic methods for aryl halide preparation. To better understand mechanistic details that limit FDH catalytic efficiency and potentially hinder their application as biocatalysts, we investigated the halogenation activity, substrate scope, crystal structures, and ligand binding of the Trp-5-halogenase AbeH and the Trp-6-halogenase BorH. Partitioning of FAD and Trp into different subunits of BorH crystals and an inability to incorporate Trp into AbeH/FAD crystals suggested that binding of flavin and Trp are negatively coupled in both proteins.

Enhanced Photocatalytic Performance of TiO@Er-Hydroxyapatite Composite for Cationic Dye and Drug Removal.

Photocatalysis heterogeneous is an essential method for water treatment. In this study, a novel photocatalytic erbium-doped hydroxyapatite-decorated titanium dioxide (TiO@Er-Hap) was obtained using the sol-gel method and applied to remove ciprofloxacin (CIP) drug and methylene blue (MB) dye contaminants. The sample's structural, physical, morphological, and photocatalytic properties were investigated.

Combined Anterior Cruciate Ligament and Anterolateral Ligament Reconstruction Using Suspensory Button Fixation.

Despite advancements in surgical techniques for anterior cruciate ligament reconstruction, some patients still experience rotational instability after surgery. Anterior cruciate ligament and anterolateral ligament reconstruction have been described using hamstring tendon autograft while preserving the insertion of the semitendinosus tendon. This article describes a combined anterior cruciate ligament and anterolateral ligament reconstruction using a hamstring tendon autograft with a suspensory button fixation.

Self-selected vs. prescribed aerobic exercise intensity: impacts on pleasure in women with obesity.

Introduction: Self-selection of aerobic training intensity is an approach that allows practitioners to develop autonomy (choice of pace), improve physical fitness, and contribute to better affective responses and training adherence. However, it is still unclear whether self-selection of training intensity in group settings is comparable to individual training conditions. The aim of the present study was to compare the effect of three aerobic training protocols on psychophysiological responses in physically inactive adult obese women.

Standardisation of Dog Sperm Morphology Classification.

Sperm morphology is an important component of dog fertility analysis. Visual examination is the gold standard technique for evaluation, but large variations within and across evaluators have been documented. Databases of sperm morphology images are valuable practical aids for training and performance testing and can improve sperm classification standardisation; however, such databases are only useful when the material is expertly classified to serve as a reference.

A meta-analysis of calcium silicate-based cements and calcium hydroxide as promoters of hard tissue bridge formation.

Background: The formation of a reparative hard tissue bridge is a key indicator of defensive pulp response and successful vital pulp treatment (VPT); however, there is a lack of comprehensive information regarding the impact of bioactive materials on hard tissue formation.

Objectives: This systematic review with meta-analyses aimed to evaluate the hard tissue bridge formation in direct pulp capping and pulpotomies in humans, comparing the use of ProRoot MTA with other calcium silicate-based cements (CSCs) and calcium hydroxide.

Methods: The search was conducted in six electronic databases, until May 2024.

Feasibility and population exposure of 5-fluorouracil using therapeutic drug monitoring (PREDICT-5FU): A multicentre clinical trial.

Aim: PREDICT-5FU aimed to document 5-fluorouracil (5FU) exposure in a cancer population and to evaluate the feasibility of 5FU and capecitabine therapeutic drug monitoring (TDM) in patients receiving standard doses and schedules.

Methods: Multicentre, prospective, observational single-arm study. Eligible adult patients received 5FU (infusional ≥24 h) or capecitabine.

RECYCLED RARE EARTH IONS FROM E-WASTE IN BOROGERMANATE GLASSES FOR MAGNETO-OPTICAL APPLICATIONS.

Magneto-optical borogermanate glasses were developed by incorporating electro-electronic waste recovered from fluorescent lamps. Glasses with a composition of (41GeO₂-25B₂O₃-4Al₂O₃-10Na₂O-20BaO)-x%wst, (x = 0, 10, 20, 30, and 50 wt% of waste) were prepared. The final chemical composition was determined, and the glasses were characterized through thermal, structural, morphological, optical, and magneto-optical analyses.

Criteria for medication reconciliation in major orthopedic surgery in high-risk patients: A consensus based on the Delphi method.

Background: Medication reconciliation is relevant in transitional care, however, given limited resources, it is necessary to identify the patients who benefit most from this activity.

Aim: To validate criteria to identify patients at high risk of medication errors undergoing major orthopedic surgery.

Method: Delphi Method in 3 phases, April to June 2023, to obtain consensus on the inclusion criteria, previously defined.