Impact of school closure during COVID-19 pandemic on school-going children in a rural population of Haryana: A qualitative study.

Background: The Government of India undertook several measures for COVID-19 pandemic, of which school closure was one. This led to sudden shift of teaching methods from classroom-based to online mode. This study aimed to understand the effects of school closure on children during lockdown period.

RNA's Dynamic Conformational Selection and Entropic Allosteric Mechanism in Controlling Cascade Protein Binding Events.

In the TAR RNA of immunodeficiency viruses, an allosteric communication exists between a distant loop and a bulge. The bulge interacts with the TAT protein vital for transactivating viral RNA, while the loop interacts with cyclin-T1, contingent on TAT binding. Through extensive atomistic and free energy simulations, we investigate TAR-TAT binding in nonpathogenic bovine immunodeficiency virus (BIV) and pathogenic human immunodeficiency virus (HIV).

Exploring the interaction of a potent anti-cancer drug Selumetinib with bovine serum albumin: Spectral and computational attributes.

The binding of drugs to plasma proteins determines its fate within the physiological system, hence profound understanding of its interaction within the bloodstream is important to understand its pharmacodynamics and pharmacokinetics and thereby its therapeutic potential. In this regard, our work delineates the mechanism of interaction of Selumetinib (SEL), a potent anti-cancer drug showing excellent effect against multiple solid tumors, with plasma protein bovine serum albumin (BSA), using methods such as absorption, steady-state fluorescence, time-resolved, fluorescence resonance energy transfer, Fourier transform infrared spectra (FTIR), circular dichroism (CD), synchronous and 3D-fluorescence, salt fluorescence, molecular docking and molecular dynamic simulations. The BSA fluorescence intensity was quenched with increasing concentration of SEL which indicates interactions of SEL with BSA.

An in-silico approach to identify bioactive phytochemicals from Thunb. As potential inhibitors of human glutathione reductase.

Cellular infections are central to the etiology of various diseases, notably cancer and malaria. Counteracting cellular oxidative stress the inhibition of glutathione reductase (GR) has emerged as a promising therapeutic strategy. , a medicinal plant known for its potent antioxidant properties, has been the focus of our investigation.

Evolution of Sequence and Structure of SARS-CoV-2 Spike Protein: A Dynamic Perspective.

Novel coronavirus (SARS-CoV-2) enters its host cell through a surface spike protein. The viral spike protein has undergone several modifications/mutations at the genomic level, through which it modulated its structure-function and passed through several variants of concern. Recent advances in high-resolution structure determination and multiscale imaging techniques, cost-effective next-generation sequencing, and development of new computational methods (including information theory, statistical methods, machine learning, and many other artificial intelligence-based techniques) have hugely contributed to the characterization of sequence, structure, function of spike proteins, and its different variants to understand viral pathogenesis, evolutions, and transmission.

Concurrent Obsessive-Compulsive Symptoms in Patients With Schizophrenia: A Retrospective Study From a Tertiary Care Centre in Sindh, Pakistan.

Introduction: The present study aimed to evaluate the proportion of concurrent symptoms of obsessive-compulsive symptoms (OCSs) among patients with schizophrenia.

Methods: A retrospective study was undertaken at the Department of Psychiatry, Jinnah Postgraduate Medical Center, Sindh, Pakistan between 1st March 2019 and 1st April 2020. All cases with diagnosed schizophrenia irrespective of gender, age, or ethnicity were eligible for the study.

Experiences of single-parent children in the current Indian context.

Background: Single-parent family structure involves only one parent responsible for rearing the child and their welfare. Among all types of families, single-parent families can have difficult individual and social experiences, which may affect both single parents as well as their children. Therefore, the present paper attempts to capture some of the lived experiences and social perceptions of single-parent children qualitatively in the current Indian context.

Computational approach to attenuate virulence of through bioinspired silver nanoparticles.

Unlabelled: In this study we aim to investigate the computational docking approach of biofabricated silver nanoparticles against virulent exoenzymes, such as ExoS and ExoY. Therefore, the synthesis and characterization of biofabricated silver nanoparticles using leaves (Pb-AgNPs) were carried out. The surface topology and functional group attachment on the surface of Pb-AgNPs were analyzed using UV-visible spectroscopy, Scanning Electron Microscopy, Fourier Transformed Infrared Spectroscopy (FTIR), and X-Ray Diffraction.

Computational Analysis of (L.) Wettst. Compounds for Drug Development against Neurodegenerative Disorders.

Aim: With several experimental studies establishing the role of Bacopa monnieri as an effective neurological medication, less focus has been employed to explore how effectively Bacopa monnieri brings about this property. The current work focuses on understanding the molecular interaction of the phytochemicals of the plant against different neurotrophic factors to explore their role and potential as potent anti-neurodegenerative drugs.

Background: Neurotrophins play a crucial role in the development and regulation of neurons.

Quantifying Mutational Response to Track the Evolution of SARS-CoV-2 Spike Variants: Introducing a Statistical-Mechanics-Guided Machine Learning Method.

The emergence of SARS-CoV-2 and its variants that critically affect global public health requires characterization of mutations and their evolutionary pattern from specific Variants of Interest (VOIs) to Variants of Concern (VOCs). Leveraging the concept of equilibrium statistical mechanics, we introduce a new responsive quantity defined as "Mutational Response Function (MRF)" aptly quantifying domain-wise average entropy-fluctuation in the spike glycoprotein sequence of SARS-CoV-2 based on its evolutionary database. As the evolution transits from a specific variant to VOC, we find that the evolutionary crossover is accompanied by a dramatic change in MRF, upholding the characteristic of a dynamic phase transition.

Exploratory Data Analysis of Genomic Sequence of Variants of SARS-CoV-2 Reveals Sequence Divergence and Mutational Localization.

Whole genome sequencing has rapidly progressed in recent years, with sequencing the SARS-CoV-2 genomes, making it a more reliable clinical tool for public health surveillance. This development has resulted in the production of a large amount of genomic data used for various types of genomic exploration. However, without a proper standard protocol, the usage of genomic data for analyzing various biological phenomena, such as mutation and evolution, may result in a propagating risk of using an unvalidated data set.

Controlled repositioning and primary osteoplasty as a treatment option for the management of unstable slipped capital femoral epiphysis.

The ideal management of an unstable slipped capital femoral epiphysis (SCFE) is still controversial. The currently available options are in-situ screw fixation with delayed corrective osteotomy, closed reduction and screw fixation and anatomic reduction by modified Dunn's procedure (MDP). We present the results of our technique of controlled re-positioning (CRP) with primary osteoplasty in which the epiphysis is repositioned to the preacute slip stage and a mini-open osteoplasty is done in the same sitting to avoid later femoro-acetabular impingement.

Factors determining loss of reduction in paediatric supracondylar humerus fractures treated by closed reduction and percutaneous pinning.

Closed reduction and percutaneous pinning is the preferred surgical intervention for paediatric supracondylar humerus fractures (SCHF). Loss of reduction (LOR) is one of the infrequent complications associated with percutaneous pinning. We aim to analyse the various factors that could lead to LOR.

Paediatric Ankle Fractures: Guidelines to Management.

Introduction: Paediatric ankle fractures represents about 5% of all paediatric fractures. It is the most common physis to be injured in the lower limb accounting to approximately 15-20% of all physeal injuries. This article reviews the literature on this common injury which still has many controversial areas and gives guidelines to management based on the existing evidence along with clinical experience gained from a Level I trauma center.

Ender's nailing of displaced tibia shaft fractures in children - A nine-year experience.

Background: Displaced fractures of the tibial shaft in children can be effectively treated with minimal complications with flexible nails. Our aim is to evaluate the outcome of displaced paediatric tibia shaft fractures treated with Ender's nails.

Materials: We retrospectively reviewed the records of all unstable tibial fractures treated between 2010 and 2018, in our institution.

Downregulation of catalase by CuO nanoparticles hypermethylation of CpG island II on the catalase promoter.

The advent of nanotechnology has led to new applications of copper as antibiotic treatment alternatives, nanocomposite coatings, catalysts, and lubricants among others. However, few studies address the impact of nano-size copper on the molecular mechanism of eukaryotic cells. Therefore, in the present study, the human hepatic cell line (WRL-68) was used to evaluate the molecular mechanism involved in the adverse effect of CuO NPs.