Bolstering Buck Fertility: The Impact of Aqueous Extract on Semen Cryopreservation and Antioxidant Defense System.

Semen cryopreservation results in sperm damage due to lipid peroxidation or oxidative stress, leading to a decrease in conception rate. The sperm damage during cryopreservation can be minimized with the use of suitable antioxidant supplements in semen diluent. Some herbs have potent antioxidant potential and can be used in semen diluent to protect the spermatozoa.

Pan msr gene deleted strain of Salmonella Typhimurium suffers oxidative stress, depicts macromolecular damage and attenuated virulence.

Salmonella encounters but survives host inflammatory response. To defend host-generated oxidants, Salmonella encodes primary antioxidants and protein repair enzymes. Methionine (Met) residues are highly prone to oxidation and convert into methionine sulfoxide (Met-SO) which compromises protein functions and subsequently cellular survival.

Porcine respiratory disease complex (PRDC) in Indian pigs: a slaughterhouse survey.

Porcine Respiratory Disease Complex (PRDC) is an unequivocally leading cause of economic losses to the pig industry. To investigate the pathogens associated with PRDC, a total of 900 lungs with gross lesions and 125 lungs with no appreciable gross lesions were collected from the abattoirs and subjected to pathological investigation for distribution of lesions/and types of exudates, as well as to molecular confirmation of bacterial and viral pathogens by PCR. The pneumonic lungs showed the higher prevalence of Mycoplasma spp.

Pathology, virulence-associated gene profiling, antimicrobial susceptibility, and pathogenicity of untypeable capsular serotypes of Pasteurella multocida isolated from slaughtered pigs of India.

Pasteurella multocida is widely distributed in all pig-rearing countries, affecting the economic viability and profitability of pig production. The present research highlights the molecular characterization and pathology of untypeable capsular serotypes of P. multocida in slaughtered pigs from prominent pig-rearing states of India.

Carrier status of in the palatine tonsils of apparently healthy slaughtered pigs of India.

is an emerging bacterial pathogen of huge economic impact to the swine industry worldwide. The information regarding the carrier status of in the slaughtered pigs along with its genetic characterization is not available in Indian pig population, which needs to be addressed for the therapeutic and preventive measures. In the present study, 563 palatine tonsils of apparently healthy slaughtered pigs were probed for the prevalence, and genetic characterization of and prevalence were found to be 15.

Non-infectious outer membrane vesicles derived from Brucella abortus S19Δper as an alternative acellular vaccine protects mice against virulent challenge.

The prime human and animal safety issues accentuate the search of promising newer alternative vaccine candidates to resolve complications associated with the live attenuated Brucella abortus strain19 (S19) vaccine. Outer membrane vesicles (OMVs S19 Δper) extracted from Brucella abortus S19Δper (S19Δper) as an alternative subunit vaccine candidate has been explored in the present study as OMVs are endowed with immunogenic molecules, including LPS and outer membrane proteins (OMPs) and do not cause infection by virtue of being an acellular entity. The LPS defective S19Δper released a higher amount of OMVs than its parent strain S19.

Evaluation of real-time PCR as an alternative for potency testing of vaccines.

The aim of the research was to evaluate real-time PCR (qPCR) as an alternate method for quantitative detection of strain 544 (S544) in the spleen of mice for potency testing of live strain 19 (S19) vaccine. and gene-based qPCR were optimized for calculating copy number. The copy number was further correlated with live count in the spleen by standard plate count (SPC) method.

Localization of Pasteurella multocida antigens in the brains of pigs naturally infected with Pasteurellosis revealing a newer aspect of pathogenesis.

Pasteurella multocida is an economically important respiratory pathogen of pigs confronting swine industry worldwide. Despite extensive research over the decades, its pathogenesis is still poorly understood. Recent reports have demonstrated the nervous system affection as a newer aspect of pathogenesis by Pasteurella multocida type B:2 in Haemorrhagic Septicemia, but there are no reports of the involvement of nervous system by P.

Association of , polymorphism with diarrhoeagenic . adhesion patterns and their jejunal expression profile in Indian Ghurrah pigs.

A total of 9 SNPs located in TFRC and ACK1 genes of SSC13q41 genomic region were examined for their association with the adhesion pattern of native Indian pigs using local isolate of diarrhoeagenic . . Phenotypic evaluation of adhesion pattern of 150 pigs revealed 116 animals positive for adhesion, whereas 34 animals had non-adhesive phenotype.

Resistance to ETEC F4/F18-mediated piglet diarrhoea: opening the gene black box.

Diarrhoea, a significant problem in pig rearing industry affecting pre- and post-weaning piglets is caused by enterotoxigenic Escherichia coli (ETEC). The ETEC are classified as per the fimbriae types which are responsible for bacterial attachment with enterocytes and release of toxins causing diarrhoea. However, genetic difference exists for susceptibility to ETEC infection in piglets.

Protective role of specific murine antibodies in inhibiting systemic proliferation of virulent strain 544 in mice and guinea pig.

Aim: The major objective of the investigation was to evaluate the hitherto uncharacterized potential of -specific antibodies to win the battle against virulent infection.

Materials And Methods: -specific immune serum was raised in mice. The antibody titer of serum was determined by standard tube agglutination test and indirect enzyme-linked immunosorbent assays (iELISA).

Novel Polymerase Spiral Reaction (PSR) for rapid visual detection of Bovine Herpesvirus 1 genomic DNA from aborted bovine fetus and semen.

Bovine herpesvirus-1 (BHV-1) is a major viral pathogen affecting bovines leading to various clinical manifestations and causes significant economic impediment in modern livestock production system. Rapid, accurate and sensitive detection of BHV-1 infection at frozen semen stations or at dairy herds remains a priority for control of BHV-1 spread to susceptible population. Polymerase Spiral Reaction (PSR), a novel addition in the gamut of isothermal techniques, has been successfully implemented in initial optimization for detection of BHV-1 genomic DNA and further validated in clinical samples.