Development of indirect ELISA and its evaluation in comparison with KOH hydrolysis and fungal culture for the immuno diagnosis of Trichophyton rubrum and Trichophyton mentagrophytes infection in humans.

Trichophyton is a keratinophilic fungus that can invade keratinized tissues of humans and cause superficial mycoses called dermatophytosis. The objective of this study was to develop and evaluate indirect ELISA in comparison with gold standard methods such as direct microscopic examination of KOH mounts and fungal culture for the diagnosis of Trichophyton infection in humans. The present investigation reports the production and partial purification of T.

Development and evaluation of indirect enzyme linked immunosorbent assay for the serological diagnosis of Microsporum canis infection in humans.

Purpose: The aim of this study was to develop and evaluate the diagnostic potential of indirect enzyme-linked immunosorbent assay (I-ELISA) for the rapid and precise diagnosis of Microsporum canis infection in humans.

Basic Procedures: The present study reports the production, partial purification and SDS-PAGE analysis of M. canis mycelial antigens and production of specific polyclonal antibodies.

Degradation of polyethylene by Trichoderma harzianum--SEM, FTIR, and NMR analyses.

Trichoderma harzianum was isolated from local dumpsites of Shivamogga District for use in the biodegradation of polyethylene. Soil sample of that dumpsite was used for isolation of T. harzianum.

A study on tubal recanalization.

Objectives: To study the various factors affecting the success rate of tubal recanalization and the reasons for failure of the procedure.

Methods: A retrospective study was conducted during 2000-2007 @ KIMS, Hubli.

Results: Of the 25 subjects who underwent tubal recanalization, 44 % of women conceived and they were >35 years of age.