Buyang Huanwu Decoction prevents hemorrhagic transformation after delayed t-PA infusion via inhibiting NLRP3 inflammasome/pyroptosis associated with microglial PGC-1α.

Ethnopharmacological Relevance: Delayed tissue-type plasminogen activator (t-PA) thrombolysis, which has a restrictive therapeutic time window within 4.5 h following ischemic stroke (IS), increases the risk of hemorrhagic transformation (HT) and subsequent neurotoxicity. Studies have shown that the NLRP3 inflammasome activation reversely regulated by the PGC-1α leads to microglial polarization and pyroptosis to cause damage to nerve cells and the blood-brain barrier.

GPR120 exacerbates the immune-inflammatory response in chicken liver by mediating acetochlor induced macrophage M1 polarization.

Acetochlor is a widely used and highly effective herbicide. Its overuse poses significant threats to biosecurity and ecological integrity, particularly affecting free-ranging birds. Data on its impact, especially mechanisms of liver toxicity in chickens, are lacking.

Effects of compound immobilized bacteria on remediation and bacterial community of PAHs-contaminated soil.

Immobilized microorganism technology is expected to enhance microbial activity and stability and is considered an effective technique for removing soil polycyclic aromatic hydrocarbons (PAHs). However, there are limited high-efficiency and stable bacterial preparations available. In this study, alkali-modified biochar (Na@CBC700) was used as the adsorption carrier, sodium alginate (SA) and polyvinyl alcohol (PVA) as embedding agents, and CaCl as the cross-linking agent to prepare immobilized Acinetobacter (CoIMB) through a composite immobilization method.

A bifunctional naphthalimide-based fluorescent probe for imaging lysosomal peroxynitrite and viscosity in living cells and zebrafish.

Peroxynitrite (ONOO) and viscosity are critical indicators of lysosome functionality, intimately linked to numerous diseases' pathophysiological processes. Hence, creating reliable analytical techniques to observe fluctuations in lysosomal ONOO and viscosity is highly important. This study presents the development of a novel naphthalimide-based fluorescent probe, Nap-Cy, specifically designed to target lysosomes and simultaneously detect both ONOO and viscosity.

Dissociation-related behaviors in mice emerge from the inhibition of retrosplenial cortex parvalbumin interneurons.

Dissociation, characterized by altered consciousness and perception, underlies multiple mental disorders, but the specific neuronal subtypes involved remain elusive. In mice, we find that dissociation-inducing doses of ketamine significantly inhibit retrosplenial cortex (RSC) parvalbumin interneurons (PV-INs), enhancing delta oscillations (1-3 Hz) and delta-gamma phase-amplitude coupling (δ-γ PAC) and inducing dissociation-like behaviors. Optogenetic inhibition of RSC PV-INs triggers delta oscillations, δ-γ PAC, and some dissociation-like behaviors without ketamine.

In-situ Oxygen-Supplying ROS Nanopurifier for Enhanced Healing of MRSA-Infected Diabetic Wounds via Microenvironment Modulation.

Hypoxia, high ROS levels and chronic inflammation are the main factors that hinder the healing of diabetic wounds. Long-term exposed wounds are prone to bacterial infection, especially MRSA infection, which exacerbates the complex wound microenvironment of diabetes and threatens patients' lives. Here, we developed a ROS nanopurifier (CSVNP), which was prepared by loading superoxide dismutase (SOD), catalase (CAT) and vancomycin into nanogels through in-situ polymerization.

Floatable and magnetic MoS/NiFeO/chitosan nanocomposite integrated melamine sponges with hybrid photothermal and photocatalytic enhancement for pollutant removal.

Magnetic chitosan-based materials with good adsorption-photocatalysis and magnetic properties have great prospect in wastewater treatment. In this paper, a floating magnetic molybdenum disulfide/NiFeO/chitosan integrated melamine sponges (m-MoS/CS@MS) was fabricated using chitosan as absorbent and adhesive, MoS and NiFeO as photocatalysts, and melamine sponge as support material. The m-MoS/CS@MS has a rich light-water-air-material interaction interface and can float on the water surface.

Role of TgVIN1 and TgPEPCK in sugar/starch and lipid metabolism pathways in Torreya grandis seeds under foliar fertilizer treatments.

Foliar fertilizers quickly replenish nutrients for plant growth, boosting production and quality. However, how this affects metabolite accumulation in fruits is unclear. In this study, the metabolome and transcriptome of Torreya grandis seeds were investigated after five different foliar fertilizer treatments.

Risk factors for deep surgical site infection following open posterior lumbar fusion: A retrospective case-control study.

This study aimed to identify risk factors for deep surgical site infection (SSI) following open posterior lumbar fusion (OPLF). We retrospectively analyzed the clinical data of patients who underwent OPLF between January 2014 and December 2022. Patients were divided into SSI and non-SSI groups according to whether deep SSI occurred following OPLF.

NUF2 is associated with cancer stem cell characteristics and a potential drug target for prostate cancer.

Background: Cancer stem cells are characterized by self-renewal, clonal tumor initiation capacity, and treatment resistance, which play essential roles in the tumor progression of prostate cancer (PCa). In this study, we aim to explore the features of cancer stemness and characterize the expression of stem cell-related genes for PCa.

Methods: We downloaded RNA-seq data and related clinical information from The Cancer Genome Atlas (TCGA) database.

[Differences in components and anti-inflammatory and analgesic activities of two phase states of Wuzhuyu Decoction].

This study rapidly identified and quantified the chemical components of the Wuzhuyu Decoction nanophase(WZYD-N) and suspension phase(WZYD-S) using ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry(UPLC-QQQ-MS/MS). Based on preliminary pharmacodynamic experiments and network pharmacology analysis, the differential anti-inflammatory and analgesic activities of WZYD-N and WZYD-S were explored to understand their pharmacodynamic basis. WZYD-N and WZYD-S were separated by differential centrifugation-dialysis, and their particle size, Zeta potential, PDI, and morphology were characterized by dynamic light scattering and transmission electron microscopy.

SARS-CoV-2 S protein disrupts the formation of ISGF3 complex through conserved S2 subunit to antagonize type I interferon response.

Unlabelled: Viral immunosuppression substantially affects the host immune response of infected patients and the protective efficacy of vaccines. Here, we found that the spike (S) protein, the major vaccine antigen of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), strongly suppresses host innate immunity by inhibiting interferon-stimulated gene (ISG) expression through both S1 and S2 subunits. Mechanistically, the S protein inhibited the formation of the classic interferon-stimulated gene factor 3 (ISGF3) complex composed of STAT1, STAT2, and IRF9 by competing with STAT2 for binding to IRF9, thereby impeding the transcription of ISGs.

Scalable Reshaping of Diamond Particles via Programmable Nanosculpting.

Diamond particles have many interesting properties and possible applications. However, producing diamond particles with well-defined shapes on a large scale is challenging because diamonds are chemically inert and extremely hard. Here, we show that air oxidation, a routine method for purifying diamonds, can be used to precisely shape diamond particles at scale.

Alzheimer's disease-associated CD83(+) microglia are linked with increased immunoglobulin G4 and human cytomegalovirus in the gut, vagal nerve, and brain.

Introduction: While there may be microbial contributions to Alzheimer's disease (AD), findings have been inconclusive. We recently reported an AD-associated CD83(+) microglia subtype associated with increased immunoglobulin G4 (IgG4) in the transverse colon (TC).

Methods: We used immunohistochemistry (IHC), IgG4 repertoire profiling, and brain organoid experiments to explore this association.

Construction of a Competency Evaluation Indicator System for Emergency Response Staff in Disease Control and Prevention Institutions - China, 2023.

What Is Already Known About This Topic?: Currently, there is no established scientific standard to guide disease control and prevention organizations in the selection of emergency response personnel. Given the growing risk of significant infectious disease outbreaks, it is imperative to develop an evaluation system for assessing emergency response capabilities.

What Is Added By This Report?: Drawing from competency theory, this study developed an assessment framework for evaluating the emergency response capabilities of staff at disease control and prevention institutions focused on major infectious diseases.

Pulmonary thromboembolism due to Mycoplasma pneumoniae in children: a case report and literature review.

Background: Mycoplasma pneumoniae (MP) is a common respiratory pathogen in children that can sometimes lead to extrapulmonary manifestations. Among these, pediatric pulmonary thromboembolism (PE) is clinically rare but carries significant implications. However, it is often underrecognized, and its diagnosis and treatment are not well understood.

A rare case report of severe aplastic anaemia caused by long-term use of zidovudine.

Background: Zidovudine is an antiviral drug used to treat acquired immune deficiency syndrome (AIDS). Anaemia is a common adverse effect of zidovudine that usually occurs in the first 2 to 4 weeks of initial treatment. Here, we describe a patient with HIV infection who developed severe aplastic anaemia 11 years after taking zidovudine.

Scalable production of ultraflat and ultraflexible diamond membrane.

Diamond is an exceptional material with great potential across various fields owing to its interesting properties. However, despite extensive efforts over the past decades, producing large quantities of desired ultrathin diamond membranes for widespread use remains challenging. Here we demonstrate that edge-exposed exfoliation using sticky tape is a simple, scalable and reliable method for producing ultrathin and transferable polycrystalline diamond membranes.

A Binary siRNA-Loaded Tetrahedral DNA Nanobox for Synergetic Anti-Aging Therapy.

Extensive accumulation of senescent cells contributes to organismal aging, and slowing down the process of cellular senescence may ameliorate age-related pathologies. Targeted inhibition of the mechanistic target of rapamycin complex 1 (mTORC1) is found to suppress the conversion of cells to senescence. The regulatory-associated protein of mTOR (Raptor), a key component of mTORC1, has been implicated as important in the aging process, and its druggability deserves to be investigated.

[Risk factors for decreased quality of life in patients with kidney stones predicted by the Chinese version of Wisconsin stone quality of life questionnaire].

Objective: To assess health related quality of life (HRQOL) in patients with kidney stones and to predict risk factors for reduced HRQOL in the patients by the Chinese version of Wisconsin stone quality of life questionnaire (C-WISQOL).

Methods: The patients with renal stones admitted to Peking University People ' s Hospital from July 2020 to June 2021 were prospectively enrolled. The inclusion criteria included the patients with renal stones aged 18-80 years and sufficient Chinese language foundation, and the exclusion criteria included the patients with internal ureteral stents, malignant tumors, sepsis, .

Construction of a 0D-2D-2D Sandwich-like Ag/g-CN/MoS Photocatalyst with Bidirectional Electron Transfer Channels for Photocatalytic Hydrogen Evolution.

Hydrogen (H) production technology has sparked a boom in research aimed at alleviating environmental pollution and the pressure of nonrenewable energy sources. A key factor in this technology is the use of efficient photocatalysts. In this work, we successfully synthesized a 0D-2D-2D sandwich-like Ag/g-CN/MoS catalyst with bidirectional electron transfer channels via a calcination-hydrothermal method.

Subclinical hypothyroidism: a new predictor of heart failure with improved ejection fraction in HFrEF patients.

Heart failure (HF) with improved ejection fraction (HFimpEF) has gradually attracted widespread attention in recent years for its better clinical prognosis. In this study, we attempted to investigate the relationship between subclinical hypothyroidism (SCH) and HFimpEF. This study retrospectively collected clinical data on patients with HF with reduced ejection fraction (HFrEF) hospitalized at the First Affiliated Hospital of USTC from March 2015 to September 2023, and divided into two groups as euthyroidism or SCH according to the baseline thyroid function.

High dietary calcium to phosphorus ratio is associated with high prevalence of kidney stone.

Kidney stones formation is a multifactorial condition and influenced, in some degree, by dietary habits. Authoritative clinical guidelines published nutritional recommendations for individuals prone to kidney stone formation. The association between dietary intake calcium to phosphorus (Ca/P) ratios and the prevalence of kidney stones is not well-established in extensive epidemiological studies.

Composite Improved Algorithm Based on Jellyfish, Particle Swarm and Genetics for UAV Path Planning in Complex Urban Terrain.

Path planning technology is of great consequence in the field of unmanned aerial vehicles (UAVs). In order to enhance the safety, path smoothness, and shortest path acquisition of UAVs undertaking tasks in complex urban multi-obstacle environments, this paper proposes an innovative composite improvement algorithm that integrates the advantages of the jellyfish search algorithm and the particle swarm algorithm. The algorithm effectively overcomes the shortcomings of a single algorithm, including parameter setting issues, slow convergence rates, and a tendency to become trapped in local optima.