Epidermal Growth Factor and Adenosine Triphosphate Induce Natrium Iodide Symporter Expression in Breast Cancer Cell Lines.

Aim: This study aims to investigate the effect of ATP, EGF and combination of those two to the Natrium Iodide Symporter (NIS) expression in MCF7, SKBR3 and HaCaT cell lines.

Methods: MCF7, SKBR3 and HaCaT cell lines were treated with ATP, EGF and combination of those two for 6, 12 and 24 hours. The expression of NIS mRNA was measured through quantitative-reverse transcription-polymerase chain reaction (qRT-PCR).

Screening and diagnosis of HIV-infection in Indonesia: one, two or three tests?

Aim: to examine among high-risk populations or patients with signs or symptoms suggesting HIV-infection, two tests or even one single test might be sufficiently accurate for diagnosis of HIV in a hospital setting in Indonesia.

Methods: we retrospectively examined the rate of false-positive results of initial HIV-tests for all subjects tested in the referral hospital for HIV in West-Java, Indonesia, between 2006 and 2008. We also calculated the positive and negative predictive value of single test results and dual-testing, based on sensitivity and specificity of commonly used methods and prevalence data from Indonesia.

Comparison of pro B-natriuretic peptide in hypertensive patients with and without diastolic dysfunction.

Aim: to evaluate whether pro BNP can be used for detection of diastolic dysfunction.

Methods: thirty nine hypertensive patients with normal systolic function, consecutively referred for echocardiography examination between October and December 2004 were recruited in the study. Diastolic dysfunction was diagnosed when echocardiographic mitral flow pattern demonstrated impaired relaxation, pseudonormalization or restrictive like patterns.