Virus Filtration Development for Adeno-Associated Virus-Based Gene Therapy Products.

Adeno-associated virus (AAV) vectors have become a leading platform for gene delivery. A major portion of gene therapy currently in clinical trials are AAV-based for a wide range of diseases. A commonly used method for AAV production is by mammalian or insect cell culture, with or without added viruses to introduce needed genetic elements for AAV production.

The effects of flux on the clearance of minute virus of mice during constant flux virus filtration.

Constant flux virus filtration experiments were conducted to evaluate minute virus of mice retention behavior of four commercial virus filters for continuous bioprocessing applications. Fluxes chosen were guided by the Peclet number and the processing logistics as well as based on the filter characteristics. At the low flux condition of 5 (LMH) when diffusive force dominates, a significant breakthrough was observed for all the filtrate fractions for the filtration of a low fouling monoclonal antibody for three of the four filters.

The effects of buffer condition on the fouling behavior of MVM virus filtration of an Fc-fusion protein.

A combined pore blockage and cake filtration model was applied to the virus filtration of an Fc-fusion protein using the three commercially available filters, F-1, F-2, and F-3 in a range of buffer conditions including sodium-phosphate and tris-acetate buffers with and without 200 mM NaCl at pH 7.5. The fouling behaviors of the three filters for the feed solutions spiked with minute virus of mice were described well by this combined model for all the solution conditions.

Transesterification of (hetero)aryl esters with phenols by an Earth-abundant metal catalyst.

Readily available and inexpensive Earth-abundant alkali metal species are used as efficient catalysts for the transesterification of aryl or heteroaryl esters with phenols which is a challenging and underdeveloped transformation. The simple conditions and the use of heterogeneous alkali metal catalyst make this protocol very environmentally friendly and practical. This reaction fills in the missing part in transesterification reaction of phenols and provides an efficient approach to aryl esters, which are widely used in the synthetic and pharmaceutical industry.

[Preparation and luminescent properties of ZnMoO4: Tb3+].

Tb3+ doped ZnMoO4 materials were synthesized by combustion method. XRD results show that the samples completely formed single ZnMoO4 phase at 700 degrees C which belongs to Trigonal, while the incorporation of Tb3+ does not affect the basic structure of ZnMoO4. The TG-DTA results show that the samples fully formed ZnMoO4 phase due to the energy absorption at about 680 C.

[Preparation and luminescence properties of EuVO4].

The material of EuVO4 was prepared by solid state reaction, and the structure, crystal form and luminescence properties of the material were characterized by X ray diffraction spectrum, infrared spectrum analysis, excitation spectrum and emission spectrum, respectively. XRD was measured at different annealing temperature and the results indicate that V2O5 and Eu2O3 began to react around 600 degrees C, and the reaction almost finished at 700 degrees C with the component of product being EuVO4. The product is in tetragonal crystallographic structure, consistent with crystal EuVO4.